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        Understanding cross-tolerance mechanism and effect of drought priming on individual heat stress and combinatorial heat and drought stress in chickpea

        Yadav Renu,Juneja Sumandeep,Kumar Rashpal,Saini Rashmi,Kumar Sanjeev 한국작물학회 2022 Journal of crop science and biotechnology Vol.25 No.5

        In northern India, chickpea experiences abrupt heat and drought stress during the late developmental stage and entails significant production loss. In the present study, the effect of heat stress and combined stress (heat and drought) was assessed in five varieties of chickpea along with the underlying mechanism of cross-tolerance by priming. Healthy seedlings (24 days old) were primed with mild drought stress and exposed to three different temperatures, i.e., 38 °C, 35 °C, and 32 °C for 12, 24, and 36 h respectively, and also in combination with drought. The damage and tolerance were evaluated based on biochemical and physiological indicators. Results indicate that significant response was observed at 35 °C as compared to 32 and 38 °C in terms of decrease in leaf water content, increased electrolyte leakage and lipid peroxidation, decreased chlorophyll content, increased accumulation of proline and total sugars, and increased antioxidative activity of superoxide dismutase, catalase, guaiacol peroxidase, ascorbate peroxidase, and glutathione reductase. The consequence of combined stress was more distinct than the individual heat stress and cannot be extrapolated from the synergistic effect of the two stresses. In addition, priming induced cross-tolerance at 35 °C by improving the membrane damage, photorespiration, photosynthetic pigment, osmolyte accumulation, and some antioxidative enzymes. On the basis of all the above parameters, PDG4 was identified as the best performing variety and tolerant to heat stress while GPF2 was the worst performing and sensitive to heat stress. It can be inferred that both severity and duration of stress are important and priming can be considered as an important tool to induce cross-tolerance in crop plants.

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        Identification of New Source of Resistance to Powdery Mildew of Indian Mustard and Studying Its Inheritance

        Joghee Nanjundan,Channappa Manjunatha,Jalli Radhamani,Ajay Kumar Thakur,Rashmi Yadav,Arun Kumar,Mohan Lal Meena,Rishi Kumar Tyagi,Devender Kumar Yadava,Dhiraj Singh 한국식물병리학회 2020 Plant Pathology Journal Vol.36 No.2

        Powdery mildew of Indian mustard (Brassica juncea), caused by Erysiphe cruciferarum, is emerging as ma- jor problem in India. All the Indian mustard cultivars presently grown in India are highly susceptible to pow- dery mildew and so far no resistance source has been reported. In this study, with an aim to identify resistant source, 1,020 Indian mustard accessions were evaluated against E. cruciferarum PMN isolate, at Wellington, The Nilgiris, Tamil Nadu, India under natural hot spot conditions. The study identified one accession (RDV 29) with complete resistance against E. cruciferarum PMN isolate for the first time, which was consistent in five independent evaluations. Genetic analysis of F1, F2 and backcross populations obtained from the cross RSEJ 775 (highly susceptible) × RDV 29 (highly resistant) for two season revealed that the resistance is governed by two genes with semi-dominant and gene dosage effect. Further, a new disease rating system using six scales (0, 1, 2, 3, 4, and 5) has also been proposed in this study to score powdery mildew based on progress of fungal growth in different plant parts of the F2 population. The outcome of this study viz. newly identified powdery mildew-resistant Indian mustard accession (RDV 29), information on inheritance of resistance and the newly developed disease rating scale will provide the base for development of powdery mildew-resistant cultivars of Indian mustard.

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