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- 저자 : Rai Anil (검색결과 3 건)
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Paul Nobin Chandra,Rai Anil,Ahmad Tauqueer,Biswas Ankur,Sahoo Prachi Misra 한국통계학회 2024 Journal of the Korean Statistical Society Vol.53 No.1
A major goal of survey sampling is finite population inference. In recent years, large-scale survey programs have encountered many practical challenges which include higher data collection cost, increasing non-response rate, increasing demand for disaggregated level statistics and desire for timely estimates. Data integration is a new field of research that provides a timely solution to these above-mentioned chal- lenges by integrating data from multiple surveys. Now, it is possible to develop a framework that can efficiently combine information from several surveys to obtain more precise estimates of population parameters. In many surveys, parameters of interest are often spatial in nature, which means, the relationship between the study variable and covariates varies across all locations in the study area and this situation is referred as spatial non-stationarity. Hence, there is a need of a sampling method- ology that can efficiently tackle this spatial non-stationarity problem and can be able to integrate this spatially referenced data to get more detailed information. In this study, a Geographically Weighted Spatially Integrated (GWSI) estimator of finite population total was developed by integrating data from two independent surveys using spatial information. The statistical properties of the proposed spatially inte- grated estimator were then evaluated empirically through a spatial simulation study. Three different spatial populations were generated having high spatial autocorrela- tion. The proposed spatially integrated estimator performed better than usual design- based estimator under all three populations. Furthermore, a Spatial Proportionate Bootstrap (SPB) method was developed for variance estimation of the proposed spa- tially integrated estimator.
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Ashok Karuppannasamy,Bhargava Chikmagalur Nagaraja,Prasad Babu Karakatti,Rohan Wishard,Manamohan Malligopal,Rai Anil,Sanjay Kumar Pradhan,Parvathy Madhusoodanan Sujatha,Kennedy John Samuel,Asokan Rama 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.1
Overuse of synthetic chemicals over a long period of time has not only resulted in control failures but also enormous ecological damage. This necessitates developing eco-friendly, effective alternatives for sustainable pest management. In this regard, potential of CRISPR/Cas9 mediated genome editing to introduce site specific mu tations that mostly resulting in loss of function has been successfully demonstrated in wide varieties of organisms including insects. This has opened a new avenue to design and implement futuristic pest management strategies like precision guided sterile insect technique (pgSIT) for an area wide suppression. This requires validation of target genes employing Ribonucleoprotein (RNP) complex containing sgRNA and Cas9 protein before under taking transgenesis for achieving pgSIT. In the present study, we have for the first time, supporting CRISPR/Cas9 mediated editing of the eye colour gene, Tryptophan 2, 3-dioxygenase of the eggplant shoot and fruit borer, Leucinodes orbonalis using RNP complex. The mutant moths of both sexes exhibited reddish brown eyed phenotype. Having established an RNP mediated editing system in L. orbonalis, the key genes involved in sex determination and spermatogenesis will be validated for developing a pgSIT system for L. orbonalis.
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Wishard Rohan,Karuppannasamy Ashok,Asokan Ramasamy,Chikmagalur Nagaraja Bhargava,Chalapathi Pradeep,Dhawane Yogi,Kumar S Sampath,Maligeppagol Manamohan,Rai Anil 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2
The Oriental fruit fly, Bactrocera dorsalis is a serious, highly invasive pest of fruits affecting global trade. Areawide management of B. dorsalis by release of sterile males obtained through irradiation suffers from the disadvantage of somatic mutations. In this regard, insect pest management in the post genome editing era poised for producing precise mutations based on CRISPR/Cas for example precision guided sterile insect technique and gene drive. Development of these technologies depend on the genes in sex determination pathway and spermatogenesis in target species. In the sex determination pathway of B. dorsalis, transformer (tra & tra2) are involved in female specific splicing of double sex (dsx). CRISPR/Cas9 mediated loss-of-function of tra has been shown to influence sex determination while it is lacking in tra2. In the present study we have edited transformer2 gene through embryonic microinjection of CRISPR/Cas9 ribonucleprotein (Cas9 + sgRNA) targeting the sixth exon. Among the emergent G0 individuals, two showed an intersex phenotype, with a male-specific dorsal bristles in the third tergite on the left side of the abdomen and a degenerate ovipositor. The third individual manifested an unique phenotype, with a complete absence of the male specific dorsal bristles but a complete degenerate ovipositor. Sequencing results revealed both addition and deletions in all the three intersexes. Additionally, molecular karyotyping of these individuals, through Maleness on Y (MoY) PCR revealed a XX karyotype for all of them. Hence, we have shown that editing of the transformer2 locus in B. dorsalis interferes with the normal sex determination.
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