Studies on Milk Allantoin and Uric Acid in Relation to Feeding Regimens and Production Performance in Buffaloes

Sikka, P.,Saxena, N.K.,Gupta, R.,Sethi, R.K.,Lall, D. Asian Australasian Association of Animal Productio 2001 Animal Bioscience Vol.14 No.11

Allantoin and uric acid were estimated in milk to study the association between the levels of these purine derivatives and milk production per day under given feeding regimens. Keeping the stage of lactation, parity and initial milk yield in view thirty lactating buffaloes were randomly selected from early lactating group. All the animals were fed 30 kg green, 2 kg straw and 5 kg concentrate mixture on per animal/day basis at basal level up to 8 1 produce. 1 kg concentrate mixture, soaked cotton seed and boiled cotton seed was fed for every 2 I milk, respectively in Group I (control), Group II and Group III animals. Average milk Allantoin and Uric acid levels were $120{\pm}11.7g/ml$ and $4.03{\pm}0.63g/ml$, respectively in milk. Cotton seed feeding enhanced the milk production significantly (p<0.01) in comparison to concentrate mixture fed control group animals. A significant difference (p<0.01) in milk allantoin levels was found over the different feeding management at higher level of production group animals. Study also revealed a significant negative correlation between the milk allantoin and production per day r=-0.43 (p<0.05).

Comprehensive identification of LMW-GS genes and their protein products in a common wheat variety

Lee, J. Y.,Beom, H. R.,Altenbach, S. B.,Lim, S. H.,Kim, Y. T.,Kang, C. S.,Yoon, U. H.,Gupta, R.,Kim, S. T.,Ahn, S. N. Springer Science + Business Media 2016 Functional & integrative genomics Vol.16 No.3

<P>Although it is well known that low-molecular-weight glutenin subunits (LMW-GS) from wheat affect bread and noodle processing quality, the function of specific LMW-GS proteins remains unclear. It is important to find the genes that correspond to individual LMW-GS proteins in order to understand the functions of specific proteins. The objective of this study was to link LMW-GS genes and haplotypes characterized using well known Glu-A3, Glu-B3, and Glu-D3 gene-specific primers to their protein products in a single wheat variety. A total of 36 LMW-GS genes and pseudogenes were amplified from the Korean cultivar Keumkang. These include 11 Glu-3 gene haplotypes, two from the Glu-A3 locus, two from the Glu-B3 locus, and seven from the Glu-D3 locus. To establish relationships between gene haplotypes and their protein products, a glutenin protein fraction was separated by two-dimensional gel electrophoresis (2-DGE) and 17 protein spots were analyzed by N-terminal amino acid sequencing and tandem mass spectrometry (MS/MS). LMW-GS proteins were identified that corresponded to all Glu-3 gene haplotypes except the pseudogenes. This is the first report of the comprehensive characterization of LMW-GS genes and their corresponding proteins in a single wheat cultivar. Our approach will be useful to understand the contributions of individual LMW-GS to the end-use quality of flour.</P>

Antitumor effect of Careya arborea against Ehrlich ascites carcinoma with reference to lipid peroxidation and enzymatic and non enzymatic antioxidant system in Swiss albino mice

R Sambath Kumar,T Sivakumar,V Senthil,N Venkateswara Murthy,V Balasubramaniam,R Kanaga Sabi,R. Shanmuga Sundram,P Perumal,U K Mazumder,M Gupta 경희대학교 융합한의과학연구소 2008 Oriental Pharmacy and Experimental Medicine Vol.8 No.2

The methanol extract of stem barks of Careya arborea Roxb. (MECA) (Family- Myrtaceae) was evaluated for antitumor activity and antioxidant status against Ehrlich’s Ascites Carcinoma (EAC) bearing Swiss albino mice. After 24 h of tumor inoculation the MECA was administered at the doses of 50, 100 and 200 mg/kg body weight/mice/day for 14 days. After the last dose and 18 h fasting mice were sacrificed. The effect of MECA on the growth of transplantable murine tumor, life span of EAC bearing hosts, hematological profiles, serum and liver biochemical parameters were estimated. The MECA showed significant (P < 0.01) decrease in ascites volume, packed cell volume and viable cell count and prolonged the life span of EAC tumor bearing mice. Hematological profiles reverted to more or less normal levels in extract treated mice. The MECA also produced protective effect by decreasing the activity of serum enzymes, bilirubin and increase the protein and uric acid levels. MECA significantly (P < 0.05) decreased the levels of lipid peroxidation, while significantly (P < 0.05) increased the levels of glutathione content, vitamin C, vitamin E, superoxide dismutase and catalase CAT. The results indicate that MECA exhibited significant antitumor and antioxidant activity in EAC bearing mice. The methanol extract of stem barks of Careya arborea Roxb. (MECA) (Family- Myrtaceae) was evaluated for antitumor activity and antioxidant status against Ehrlich’s Ascites Carcinoma (EAC) bearing Swiss albino mice. After 24 h of tumor inoculation the MECA was administered at the doses of 50, 100 and 200 mg/kg body weight/mice/day for 14 days. After the last dose and 18 h fasting mice were sacrificed. The effect of MECA on the growth of transplantable murine tumor, life span of EAC bearing hosts, hematological profiles, serum and liver biochemical parameters were estimated. The MECA showed significant (P < 0.01) decrease in ascites volume, packed cell volume and viable cell count and prolonged the life span of EAC tumor bearing mice. Hematological profiles reverted to more or less normal levels in extract treated mice. The MECA also produced protective effect by decreasing the activity of serum enzymes, bilirubin and increase the protein and uric acid levels. MECA significantly (P < 0.05) decreased the levels of lipid peroxidation, while significantly (P < 0.05) increased the levels of glutathione content, vitamin C, vitamin E, superoxide dismutase and catalase CAT. The results indicate that MECA exhibited significant antitumor and antioxidant activity in EAC bearing mice.

Physical Factors in Relation to Conidial Germination in Alternaria porri, the Incitant of Purple Blotch of Onion

R.B.L. 굽타,V.N. 파닥,O.P. 베르마,Gupta, R.B.L.,Pathak, V.N.,Verma, O.P. Korean Society of Applied Entomology 1985 한국식물보호학회지 Vol.24 No.3

Influence of temperature, relative humidity, spore washing and spore drying on conidial germination of Alternaria porri(Ell.) Cif. was studied. Maximum conidial germination occurred at 100% relative humidity prevailing for 6 hours or more at $25^{\circ}C$. Conidial germination decreased with increase in number of spore washings. Drying of conidia for more than half an hour caused significant decrease in germination. In all the experiments, conidial germinatio increased with increase in incubation period.

Endomorphism Rings of Finite Length Injective Modules

R. N. Gupta,Dinesh Khurana 경북대학교 자연과학대학 수학과 2003 Kyungpook mathematical journal Vol.43 No.2

We prove the existence of a finite length injective right module whose endo-morphism ring is not right Artinian.

Antitumor effect of Careya arborea against Ehrlich ascites carcinoma with reference to lipid peroxidation and enzymatic and non enzymatic antioxidant system in Swiss albino mice

Kumar, R Sambath,Sivakumar, T,Senthil, V,Murthy, N Venkateswara,Balasubramaniam, V,Sabi, R Kanaga,Sundram, R. Shanmuga,Perumal, P,Mazumder, U K,Gupta, M Kyung Hee Oriental Medicine Research Center 2008 Oriental pharmacy and experimental medicine Vol.8 No.2

The methanol extract of stem barks of Careya arborea Roxb. (MECA) (Family- Myrtaceae) was evaluated for antitumor activity and antioxidant status against Ehrlich's Ascites Carcinoma (EAC) bearing Swiss albino mice. After 24 h of tumor inoculation the MECA was administered at the doses of 50, 100 and 200 mg/kg body weight/mice/day for 14 days. After the last dose and 18 h fasting mice were sacrificed. The effect of MECA on the growth of transplantable murine tumor, life span of EAC bearing hosts, hematological profiles, serum and liver biochemical parameters were estimated. The MECA showed significant (P < 0.01) decrease in ascites volume, packed cell volume and viable cell count and prolonged the life span of EAC tumor bearing mice. Hematological profiles reverted to more or less normal levels in extract treated mice. The MECA also produced protective effect by decreasing the activity of serum enzymes, bilirubin and increase the protein and uric acid levels. MECA significantly (P < 0.05) decreased the levels of lipid peroxidation, while significantly (P < 0.05) increased the levels of glutathione content, vitamin C, vitamin E, superoxide dismutase and catalase CAT. The results indicate that MECA exhibited significant antitumor and antioxidant activity in EAC bearing mice.

EFFECT OF SUPPLEMENTING UREA MOLASSES MINERAL BLOCK LICKS ON BACTERIAL PRODUCTION RATE IN THE RUMEN OF CROSSBRED CALVES

Garg, M.R.,Gupta, B.N. Asian Australasian Association of Animal Productio 1992 Animal Bioscience Vol.5 No.3

Sixteen crossbred (Sahiwal $\times$ Holstein) male rumen fistulated calves of 18 to 24 months of age were randomly divided into four groups of four animal, each. Animals in all the groups were fed wheat straw ad lib as basal roughage. However, the animals in group I were fed concentrate mixture at maintenance level, whereas, the animals in groups II, III and IV had free access to existing, modified (A) and modified (B) urea molasses mineral block licks respectively. Daily wheat straw intake (kg) was significantly (p<0.01) higher in groups II ($4.20{\pm}0.13$), III ($4.07{\pm}0.16$) and IV ($4.22{\pm}0.20$) as compared to group I ($3.21{\pm}0.14$). Total N and TCA precistrained rumen liquor) was significantly higher in groups II ($22.36{\pm}0.25$), III ($21.63{\pm}0.25$) and IV ($21.77{\pm}0.55$) as compared to group I ($18.31{\pm}0.41$). Bacterial production rate (g/day and g/kg digestible organic matter intake) were non-significantly different amongst groups I ($214.4{\pm}13.28;\;85.38{\pm}3.69$); II ($198.7{\pm}5.70;\;86.17{\pm}3.53$); III ($214.4{\pm}8.19;\;96.15{\pm}2.16$) and IV ($218.2{\pm}10.62;\;94.44{\pm}5.52$). Similarly, percent efficiency of N incorporation into bacterial protein was not found significantly different amongst groups I, II, III and IV. These studies indicate that when concentrate mixture (upto maintenance level) in the diet of ruminants was replaced with UMMB licks, various N fraction in SRL and efficiency of bacterial production rates in the rumen were not affected.

EFFECT OF SUPPLEMENTING UREA MOLASSES MINERAL BLOCK LICK TO STRAW BASED DIET ON DM INTAKE AND NUTRIENT UTILIZATION

Garg, M.R.,Gupta, B.N. Asian Australasian Association of Animal Productio 1992 Animal Bioscience Vol.5 No.1

Twelve male crossbred calves of 18 months of age were divided into two groups of six each. Animals in both the groups were fed wheat straw ad lib. However, animals in group II had free access to urea molasses mineral block (UMMB) lick. Straw DM intake (kg/100 kg B. Wt. and $g/w^{0.75}\;kg$) was significantly (p < 0.01) higher in group II ($1.95{\pm}0.06$; $75.55{\pm}1.79$) as compared to group I ($1.27{\pm}0.08$; $48.77{\pm}2.43$). Straw DM digestibility coefficient was not significantly different in groups I and II. However, DOMI (kg/100 kg B. Wt.) was significantly (p < 0.01) higher in group II ($0.986{\pm}0.05$) as compared to group I ($0.615{\pm}0.03$). Digestibility coefficient of DM, OM and CP were significantly higher in group II as compared to group I. However, digestibility coefficients of EE, CF and NFE were non-significantly different between the two groups. Animals in group II exhibited significantly (p < 0.01) higher and positive N, Ca and P balances as compared to group I which exhibited negative balances. Total-N, ammonia-N and urea-N in the blood plasma of animals in group II were significantly (p < 0.01) higher as compared to group I.

NUTRIENT UTILIZATION, GROWTH RATE AND BODY COMPOSITION OF CROSSBRED CALVES SUPPLEMENTED WITH UREA MOLASSES MINERAL BLOCK LICKS

Garg, M.R.,Gupta, B.N. Asian Australasian Association of Animal Productio 1993 Animal Bioscience Vol.6 No.3

Twenty male crossbred ($Sahiwal{\times}HF$) calves of about 6-9 months of age were divided into four groups of five animals each. All the animals were offered wheat straw ad lib. As the basal feed. However, animals in group I were fed concentrate mixture while the animals in groups II, III and IV had free access to urea molasses mineral block (UMMB) lick (I), (II) and (III) respectively as a partial substitute of concentrate mixture. The average concentrate offered (kg/day) to the animals was significantly (p<0.01) higher in group I ($2.69{\pm}0.18$) compared to groups II ($1.76{\pm}0.15$), III ($1.70{\pm}0.06$) and IV ($1.65{\pm}0.12$). The UMMB lick consumed was non-significantly different amongst groups I ($535.40{\pm}38.14$), II ($525.60{\pm}31.82$), III ($551.00{\pm}38.49$) and IV ($548.80{\pm}45.46$). Except ether extract, the digestibility coefficients of CP, ADF and NDF were non-significantly different in different groups. Similarly, N balance (g/day) and percent N retention of intake was not affected in different groups on supplementation of UMMB lick. Body composition of animals was similar in different groups supplemented with either concentrate mixture or concentrate mixture and UMMB licks. It may be concluded from these studies that UMMB lick can partially replace the concentrate mixture in the diet of growing calves without affecting the growth rate, nutrient utilization and body composition. The UMMB lick, thus, can form a part of the ration economically in the diet of growing ruminants especially in developing countries.

양파검은무늬병균의 포자발아에 미치는 물리적 요인

R.B.L. GUPTA,V.N. PATHAK,O.P. VERMA 한국응용곤충학회 1985 한국응용곤충학회지 Vol.24 No.3

Influence of temperature, relative humidity, spore washing and spore drying on conidial germination of Alternaria porri(Ell.) Cif. was studied. Maximum conidial germination occurred at 100% relative humidity prevailing for 6 hours or more at . Conidial germination decreased with increase in number of spore washings. Drying of conidia for more than half an hour caused significant decrease in germination. In all the experiments, conidial germinatio increased with increase in incubation period.