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RISS 인기검색어
- 검색키워드
- 저자 : R. D. Kamm (검색결과 3 건)
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A high-throughput microfluidic assay to study neurite response to growth factor gradients
Kothapalli, Chandrasekhar R.,van Veen, Ed,de Valence, Sarra,Chung, Seok,Zervantonakis, Ioannis K.,Gertler, Frank B.,Kamm, Roger D. Royal Society of Chemistry 2011 Lab on a chip Vol.11 No.3
<P>Studying neurite guidance by diffusible or substrate bound gradients is challenging with current techniques. In this study, we present the design, fabrication and utility of a microfluidic device to study neurite guidance under chemogradients. Experimental and computational studies demonstrated the establishment of a steep gradient of guidance cue within 30 min and stable for up to 48 h. The gradient was found to be insensitive to external perturbations such as media change and movement of device. The effects of netrin-1 (0.1–10 µg mL<SUP>−1</SUP>) and brain pulp (0.1 µL mL<SUP>−1</SUP>) were evaluated for their chemoattractive potential on neurite turning, while slit-2 (62.5 or 250 ng mL<SUP>−1</SUP>) was studied for its chemorepellant properties. Hippocampal or dorsal root ganglion (DRG) neurons were seeded into a micro-channel and packed onto the surface of a 3D collagen gel. Neurites grew into the matrix in three dimensions, and a gradient of guidance cue was created orthogonal to the direction of neurite growth to impact guidance. The average turning angle of each neurite was measured and averaged across multiple devices cultured under similar conditions to quantify the effect of guidance cue gradient. Significant positive turning towards gradient was measured in the presence of brain pulp and netrin-1 (1 µg mL<SUP>−1</SUP>), relative to control cultures which received no external guidance cue (<I>p</I> < 0.001). Netrin-1 released from transfected fibroblasts had the most positive turning effect of all the chemoattractive cues tested (<I>p</I> < 0.001). Slit-2 exhibited strong chemorepellant characteristics on both hippocampal and DRG neurite guidance at 250 ng mL<SUP>−1</SUP> concentration. Slit-2 also showed similar behavior on DRG neuron invasion into 3D collagen gel (<I>p</I> < 0.01 relative to control cultures). Taken together, the results suggest the utility of this microfluidic device to generate stable chemogradients for studying neurobiology, cell migration and proliferation, matrix remodeling and co-cultures with other cell lines, with potential applications in cancer biology, tissue engineering and regenerative medicine.</P> <P>Graphic Abstract</P><P>We describe a three-channel microfluidic device to study the role of chemogradients on neurite outgrowth and guidance in 3D scaffolds, using experimental and computational studies. The stable chemogradients in these devices could also be used to screen potential drugs for neuron pathway regeneration under disease/ injury conditions, cell migration and cell–cell interactions. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c0lc00240b'> </P>
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우주비행 직후 인체 심혈관계의 혈류역학적 변화에 대한 수치적 연구
심은보(E. B. Shim),고형종(H. J. Ko),T. Heldt,R. D. Kamm,R. G. Mark 한국전산유체공학회 2000 한국전산유체공학회 학술대회논문집 Vol.2000 No.10
Orthostatic stress in human cardiovascular system following spaceflight remains a critical problem in the current lifescience space program. The study presented in this paper is part of an<br/> ongoing effort to use mathematical models to investigate the effects of gravitational stresses on the cardiovascular system of normals and microgravity adapted individuals. We employ a twelve compartment lumped parameter representation of the hemodynamic system coupled to setpoint models of the arterial baroreflex and the cardiopulmonary reflex to investigate the transient response of heart rate to orthostatic stress. We simulate current hypotheses concerning the mechanisms underlying postspaceflight orthostatic intolerance over a range of physiologically reasonable values and compare the simulations to astronaut stand test data pre-and postflight.
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