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Yung O . Shin,Elaine M . Elder,R . Wayne Atchison 대한바이러스학회 1981 Journal of Bacteriology and Virology Vol.11 No.1
Enzyme immunoassay (EIA) techniques were modified for the direct detection of influenza soluble antigens in patient specimens. Twenty-four available clinical specimens, previously tested by irnmunofluorescence(IIF) on cell culture isolates and by egg isolation (EGG) techniques, were retrospectively tested by 2 different methods of EIA, I.e., direct sorption type, EIA (DS), and double antibody, EIA (DA), EIA (DS) showed. 83.3% (20/24) agreement with IIF and 58.3% (14/24) agreement with EGG. EIA (DS) detected more positive specimens (50% 12/24) than EIA (DA) (36,8%, 8/24). EIA (DS) data showed the highest-agreement (91.7%, 22/24) with data obtained from both IIF and EGG; 2 specimens gave different results from those seen by IIF and EGG. With the advantages of its sensitivity, rapidity and sirnplicity, EIA test for the detection of influenza soluble antigen would be an important supplement to other standard techniques used in influenzal diagnosis.