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- 저자 : Que Youxiong (검색결과 1 건)
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Wang Hengbo,Feng Meichang,Zhong Xiaoqiang,Yu Qing,Que Youxiong,Xu Liping,Guo Jinlong 한국유전학회 2023 Genes & Genomics Vol.45 No.1
Background Calmodulin (CaM) plays an essential role in binding calcium ions and mediating the interpretation of Ca2+ signals in plants under various stresses. However, the evolutionary relationship of CaM family proteins in Saccharum has not been elucidated. Objective To deduce and explore the evolution and function of Saccharum CaM family. Methods A total of 104 typical CaMs were obtained from Saccharum spontaneum and other 18 plant species. The molecular characteristics and evolution of those CaM proteins were analyzed. A typical CaM gene, ScCaM1, was subsequently cloned from sugarcane (Saccharum spp. hybrid). Its expression patterns in different tissues and under various abiotic stresses were assessed by quantitative real-time PCR. Then the green fluorescent protein was used to determine the subcellular localization of ScCaM1. Finally, the function of ScCaM1 was evaluated via heterologous yeast expression systems. Results Three typical CaM members (SsCaM1, SsCaM2, and SsCaM3) were identified from the S. spontaneum genome database. CaMs were originated from the two last common ancestors before the origin of angiosperms. The number of CaM family members did not correlate to the genome size but correlated with allopolyploidization events. The ScCaM1 was more highly expressed in buds and roots than in other tissues. The expression patterns of ScCaM1 suggested that it was involved in responses to various abiotic stresses in sugarcane via different hormonal signaling pathways. Noteworthily, its expression levels appeared relatively stable during the cold exposure in the cold-tolerant variety but significantly suppressed in the cold-susceptible variety. Moreover, the recombinant yeast (Pichia pastoris) overexpressing ScCaM1 grew better than the wild-type yeast strain under cold and oxidative stresses. It was revealed that the ScCaM1 played a positive role in reactive oxygen species scavenging and conferred enhanced cold and oxidative stress tolerance to cells. Conclusion This study provided comprehensive information on the CaM gene family in Saccharum and would facilitate further investigation of their functional characterization.
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