RISS 학술연구정보서비스

검색
다국어 입력

http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.

변환된 중국어를 복사하여 사용하시면 됩니다.

예시)
  • 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
  • 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
닫기
    인기검색어 순위 펼치기

    RISS 인기검색어

      • 무료
      • 기관 내 무료
      • 유료
      • KCI등재

        Mutation breeding of nuclease p1 production in Penicillium citrinum by low-energy ion beam implantation

        Qinting He,Hanjie Ying,Nan Li,Xiaochun Chen,Qi Ye,Jianxin Bai,Jian Xiong 한국화학공학회 2011 Korean Journal of Chemical Engineering Vol.28 No.2

        Nuclease p1 is an important enzyme in the nucleotide industry that is used to hydrolyze nucleic acid into nucleotides. To improve enzyme activity, Penicillium citrinum, a nuclease p1 producing strain, was mutated by lowenergy Nitrogen ion beam implantation at an energy level of 15 keV and a dose ranging from 1×10^(15)-1×10^(16) ions/cm^2. The mutant strain designated as N409 was obtained with a high yield of nuclease p1. The activity of nuclease p1 was 421 U/mL from the mutant strain N409, which was increased by 86% compared with the control. The fermentation kinetics of nuclease p1 by the mutant strain N409 was studied in a 30 L external airlifting bioreactor. A model was proposed using the logistic equation for microbial growth, the Luedeking-Piret equation for product formation and a Luedeking-Piret-like equation for substrate uptake. The results predicted from the model were in good agreement with the experimental observations.

      연관 검색어 추천

      이 검색어로 많이 본 자료

      활용도 높은 자료

      해외이동버튼