Study on Large Straight Blade Wide Impeller

Qi Chun Song,Dan Hui Jie,Yang Yang Zhe,Dong Kang Tian 대한기계학회 1997 International Conference on Fluid Machinery Vol.1 No.1

HIF-1α and GLUT1 Gene Expression is Associated with Chemoresistance of Acute Myeloid Leukemia

Song, Kui,Li, Min,Xu, Xiao-Jun,Xuan, Li,Huang, Gui-Nian,Song, Xiao-Ling,Liu, Qi-Fa Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4

Aims: Much evidence suggests that increased glucose metabolism in tumor cells might contribute to the development of acquired chemoresistance. However, the molecular mechanisms are not fully clear. Therefore, we investigated a possible correlation of mRNA expression of HIF-$1{\alpha}$ and GLUT1 with chemoresistance in acute myeloid leukemia (AML). Methods: Bone marrow samples were obtained from newly diagnosed and relapsed AML (M3 exclusion) cases. RNA interference with short hairpin RNA (shRNA) was used to stably silence GLUT1 or HIF-$1{\alpha}$ gene expression in an AML cell line and HIF-$1{\alpha}$ and GLUT1 mRNA expression was measured by real-time quantitative polymerase chain reaction assay (qPCR). Results: High levels of HIF-$1{\alpha}$ and GLUT1 were associated with poor responsiveness to chemotherapy in AML. Down-regulation of the expression of GLUT1 by RNA interference obviously sensitized drug-resistant HL-60/ADR cells to adriamycin (ADR) in vitro, comparable with RNA interference for the HIF-$1{\alpha}$ gene. Conclusions: Our data revealed that over-expression of HIF-$1{\alpha}$ and GLUT1 might play a role in the chemoresistance of AML. GLUT1 might be a potential target to reverse such drug resistance.

사회적 지지, 외로움, 다문화 적응 및 학업적응 간의 구조적 관계 : 재한 중국인 유학생을 중심으로

SONG QI,안도희 한국국제문화교류학회 2023 문화교류와 다문화교육 Vol.12 No.6

This study explored the structural relationships between social support, cross-cultural adaptation, loneliness, and academic adjustment among Chinese international students studying in South Korea. The final analysis involved 355 Chinese international students enrolled in universities located in Seoul, Korea. The results of this study are as follows: Chinese international students in Korea perceived significant positive correlations between social support, cross-cultural adaptation, and academic adjustment. On the other hand, loneliness showed a significant negative correlation with these variables. Upon analyzing differences based on friendship patterns (i.e., number of friends and frequency of interactions with friends), it was evident that perceptions of social support, loneliness, cross-cultural adaptation, and academic adjustment varied. Loneliness and cross-cultural adaptation were found to fully mediate the relationship between social support and academic adjustment. It is anticipated that these findings can serve as foundational data for the educational field in seeking strategies to enhance the academic adjustment of Chinese international students in South Korea.

Effects of near-fault loading and lateral bracing on the behavior of RBS moment connections

Qi-Song “Kent” Yu,Chia-Ming Uang 국제구조공학회 2001 Steel and Composite Structures, An International J Vol.1 No.1

An experimental study was conducted to evaluate the effects of loading sequence and lateral bracing on the behavior of reduced beam section (RBS) steel moment frame connections. Four full-scale moment connections were cyclically tested-two with a standard loading history and the other two with a near-fault loading history. All specimens reached at least 0.03 radian of plastic rotation without brittle fracture of the beam flange groove welds. Two specimens tested with the nearfault loading protocol reached at least 0.05 radian of plastic rotation, and both experienced smaller buckling amplitudes at comparable drift levels. Energy dissipation capacities were insensitive to the types of loading protocol used. Adding a lateral bracing near the RBS region produced a higher plastic rotation; the strength degradation and buckling amplitude were reduced. A non-linear finite element analysis of a one-and-a-half-bay beam-column subassembly was also conducted to study the system restraint effect. The study showed that the axial restraint of the beam could significantly reduce the strength degradation and buckling amplitude at higher deformation levels.

Roles of E-Cadherin (CDH1) Genetic Variations in Cancer Risk: a Meta-analysis

Deng, Qi-Wen,He, Bang-Shun,Pan, Yu-Qin,Sun, Hui-Ling,Xu, Ye-Qiong,Gao, Tian-Yi,Li, Rui,Song, Guo-Qi,Wang, Shu-Kui Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8

E-Cadherin (CDH1) genetic variations may be involved in invasion and metastasis of various cancers by altering gene transcriptional activity of epithelial cells. However, published studies on the association of CDH1 gene polymorphisms and cancer risk remain contradictory, owing to differences in living habits and genetic backgrounds. To derive a more better and comprehensive conclusion, the present meta-analysis was performed including 57 eligible studies of the association between polymorphisms of CDH1 gene promoter -160 C>A, -347 G>GA and 3'-UTR +54 C>T and cancer risk. Results showed that these three polymorphisms of CDH1 were significantly associated with cancer risk. For -160 C>A polymorphism, -160A allele carriers (CA and CA+AA) had an increased risk of cancer compared with the homozygotes (CC), and the similar result was discovered for the -160A allele in the overall analyses. In the subgroup analyses, obvious elevated risk was found with -160A allele carriers (AA, CA, CA+AA and A allele) for prostate cancer, while a decreased colorectal cancer risk was shown with the AA genotype. For the -347 G>GA polymorphism, the GAGA genotype was associated with increased cancer risk in the overall analysis with homozygous and recessive models. In addition, results of subgroup analysis indicated that the elevated risks were observed in colorectal cancer and Asian descendants. For +54 C>T polymorphism, a decreased risk of cancer was found in heterozygous, dominant and allele models. Moreover, +54T allele carriers (CT, CT+TT genotype and T allele) showed a potential protective factor in gastric cancer and Asian descendants.

Identification of nucleolar and coiled-body phosphoprotein 1 (NOLC1) minimal promoter regulated by NF-κB and CREB

( Xue Song Gao ),( Qi Wang ),( Wei Li ),( Biao Yang ),( Hao Song ),( Wei Ju ),( Shu Nai Liu ),( Jun Cheng ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.1

Nucleolar and coiled-body phosphoprotein 1 (NOLC1) is a phosphoprotein that transiently associates with the mature nucleolar H/ACA and C/D box small nucleolar ribonucleoproteins (snoRNPs). Several lines of evidence indicate that NOLC1 plays an important role in the synthesis of rRNA and the biosynthesis of ribosomes. In the present study, we examined the transcriptional regulation mechanisms that govern the expression of NOLC1. We first performed functional dissection of the NOLC1 promoter. We demonstrated that transcription factors NF-κB and CREB could bind to the minimal NOLC1 promoter. This was demonstrated by electrophoretic mobility shift assays and chromatin immunoprecipitation. Mutagenesis and overexpression assays revealed that NF-κB and CREB positively regulated the NOLC1 promoter. These findings may provide new insight into the mechanisms that regulate NOLC1 expression. [BMB reports 2011; 44(1): 70-75]

Function and Oligomerization Study of the Leucine Zipper-Like Domain in P13 from Leucania separata Multiple Nuclear Polyhedrosis Virus

( En Qi Du ),( Lun Guang Yao ),( Hua Xu ),( Song Ya Lu ),( Yi Peng Qi ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.2

码头作业费(THC)的争议分析及合理性建议

宋?明(SONG QI-MING),?慧琴(HUIQIN, ZHU) 동아대학교 법학연구소 2020 國際去來와 法 Vol.- No.29

THC refers to the surcharge charged by the carrier from the shipper. In order to make up for the additional expenses incurred by the carrier from the container terminal to the ships side, it has always been a very controversial issue. The problem of the collection of wharf operating fees in China also dates back to the 1980s. For China, the reduction of wharf operating fees can reduce the burden of Chinese import and export enterprises by about 4.6 billion every year. But at the same time, with the development of Chinese liner companies, wharf operating fees have greatly increased the income of Chinese liner companies, and increasing or reducing wharf operating fees will damage the entry and exit of China. The overall income of the mouth, the wharf operation fee is like a double-edged sword, so that the establishment and reform of the laws and regulations are in a dilemma. This paper discusses and analyzes the generation, background and rationality of wharf operating fee, and concludes that the collection of wharf operating fee is essentially a dispute between the interests of both ship and cargo, and should abide by the basic law of the market. at the same time, it is found that the charging standard of wharf operating fee in China is lack of standardization and transparency. Wharf operating fee is the product of the times and the result of the game between the two freighters. It has the rationality of the times and should continue to play the role of the market. Finally, on this basis, this paper puts forward some reasonable suggestions for collecting wharf operating fees. Speed up the improvement of shipping laws and regulations, at the same time draw lessons from domestic and foreign experience, deal with the relationship between the government and the market, for the collection and legal norms of wharf operating fees, continue to take the market as the leading factor, find problems and solve problems, the government should do its auxiliary normative role, so that the normative role of laws and regulations is more reasonable and fair.

Decreased Expression of LKB1 Correlates with Poor Prognosis in Hepatocellular Carcinoma Patients Undergoing Hepatectomy

Huang, Yue-Han,Chen, Zhen-Kun,Huang, Ka-Te,Li, Peng,He, Bin,Guo, Xu,Zhong, Jun-Qiao,Zhang, Qi-Yu,Shi, Hong-Qi,Song, Qi-Tong,Yu, Zheng-Ping,Shan, Yun-Feng Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

Aim: To study any correlation of LKB1 expression with prognosis in hepatocellular carcinoma (HCC) cases. Methods: A total of 70 HCC patients and 20 primary intrahepatic stone patients in the first affiliated hospital of Wenzhou Medical College were enrolled in this study. LKB1 expression was detected by immunohistochemistry. Patients were followed-up and prognostic factors were evaluated. Result: LKB1 expression was decreased in the HCC samples. Loss of LKB1 expression in HCC was significantly related to histologic grade (P=0.010), vascular invasion (P=0.025) and TMN stage (P=0.011). Patients showing negative LKB1 expression had a significantly shorter disease-free and overall survival than those with positive expression (P = 0.001, P=0.000, respectively). Multivariate Cox regression analysis indicated that LKB1 expression level was an independent factor of survival (P = 0.033). Conclusion: HCC patients with decreased expression LKB1 have a poor prognosis. The loss of LKB1 expression is correlated with a lower survival rate.

Characterization of a Novel cry1-Type Gene from Bacillus thuringiensis subsp. alesti Strain LY-99

Qi, Xu Feng,Li, Ming Shun,Choi, Jae-Young,Roh, Jong-Yul,Song, Ji Zhen,Wang, Yong,Jin, Byung-Rae,Je, Yeon-Ho,Li, Jian Hong Korean Society of Sericultural Science 2009 International Journal of Industrial Entomology Vol.18 No.1

B. thuringiensis strain LY-99 belonging to subsp. alesti (H3a3c), was isolated from Chinese tobacco warehouse and showed significantly high toxicity to Plutella xylostella. For the identification of the cry1-type genes from B. thuringiensis LY-99, an extended multiplex PCRrestriction fragment length polymorphism (PCRRFLP) method was established by using two pairs of universal primers based on the conserved regions of the cry1-type genes to amplify around 2.4 kb cry1-type gene fragments. Then the DNA fragment was cloned into pGEM-T Easy vector and digested with EcoRI and EcoRV enzymes. Through this method, a known cry1-type gene was successfully identified from the reference strain, B. thuringiensis subsp. alesti. In addition, the RFLP patterns revealed that B. thuringiensis LY-99 included a novel cry1A-type gene in addition to cry1Aa, cry1Ac, cry1Be and cry1Ea genes. The novel cry1A-type gene was designated cry1Ah2 (Genbank accession No DQ269474). An inverse PCR method was used to amplify the flank regions of cry1Ah2 gene. Finally, 3143 bp HindIII fragment from B. thuringiensis LY-99 plasmid DNA including 5' region and partial ORF was amplified, and sequence analysis revealed that cry1Ah2 gene from LY-99 showed 89.31% of maximum sequence similarity with cry1Ac1 crystal protein gene. In addition, the deduced amino acid sequence of Cry1Ah2 protein shared 87.80% of maximum identity with that of Cry1Ac2. This protein therefore belongs to a new class of B. thuringiensis crystal proteins.