송어를 감염원으로 한 광절열두조충 인체감염

정평림,이규재,양용석,안영겸 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1996 Journal of biomedical laboratory sciences Vol.2 No.2

최근 한국에 있어서 광절열두조충 감염예가 가끔 보고되고 있다. 저자등은 1994년 10월 강원도 춘천시 거주 남녀 부부가 함께 담수산 송어회를 먹고 감염된 2예에 대하여 보고하는 바이다. 구충은 하제(下濟)로서 Magnesium sulfate 15gm, 구충제 Praziquantel 15mg/kg를 투여 하였다. 제1예는 인OO, 43세 남자, 69kg의 건강체였으며, 구충하여 얻은 충체는 길이 6.65m, 폭 8-13mm였다. 제2예는 최OO, 39세 여자, 56kg으로 소화불량, 복부 불쾌감을 호소하였고, 구충하여 얻은 충체는 5.50m, 폭 8-13mm이었다. 2예 모두에서 두부는 검출 되지 않았다. 구충하여 얻은 충체의 외관상 형태, 자궁의 선회, 자궁분지(uterine loops)의 수, 생식공의 위치와 절단표본상 음경낭과 저정낭의 형태, 질의 위치, 자궁구의 위치등과 충란을 광학현미경으로 관찰 한 형태, 크기 및 SEM으로 관찰 한 표피의 작은 점각(pits)의 유무, 분포등 형태학적인 특징등으로 Diphyllobothrium latum으로 동정하였다. 이번의 인체 감염은 담수산 송어회가 원인이 된 것으로 믿어지는 예이다. A total of 31 human diphyllobothriasis cases have been reported in Korea. Authors experienced two more human cases of Diphyllobothrium latum infection, especially due to eating raw freshwater trout. Two cases were husband and wife in a famil residing in Chunchon city, Kangwon-do, who raw fish in October 1994. A 43-year old male(husband) was 69kg in body weight and healthy. A worm (6.65m in length; 8-l3mm in width) was expelled from him anthelmintic treatment. The second case(wife) was a 39-year old female who weighted She complained about gastrointestinal trouble and abdominal discomfort. A worm (5.50m in length; 9-l3mm in width) was Obtained her after anthelmintic treatment. No scolex was collected from the two worms. Two patients were administrated with praziquantel(l5mg/kg of body weight) and l5gm of magnesium sulfate as a purgative. Two worms identified as D. latum, based on the following biological characters: extermal morphologies, coiling of uterus, the number of uterine loops, position of genital opening, morphologies of cirrus and cirrus sac and seminal vesicle on the histological sections, positions of vagina and uterine pore, distribution patterns of vitellaria, and microscopical and SEM morphologies of the egg.

한강, 섬진강, 만경강 수계에 존재하는 대형 무척추동물의 분포

정평림,정재춘 연세대학교 보건과학연구소 1993 보건과학논집 Vol.3 No.-

病原性 原生動物類의 種鑑別을 爲한 螢光抗體法의 應用

鄭坪林,崔信錫,辛昌男 충남대학교 자연과학연구소 1977 學術硏究誌 Vol.4 No.1

原生動物門中 鞭謀蟲類인 Trichomonas vaginalis, Trichomonas muris, Trichomonas tenax, Trypanosoma cruzi 根足蟲類인 Entamoeba histolyca의 local strain (Ys-27)과 axenic strain (HK-9), Entamoeba muris, Acanthamoeba sp. 그리고 胞子蟲類인 Toxop lasma gondii의 RH 및 Beverley strain을 對象으로 하였다. 上記 原生動物類를 種別로 家免에 注入하여 얻은 亢血淸으로 間接螢光抗體法과 制動反應을 竝行하여 얻은 種別 抗原特異性을 比較한 結果 다음과 같은 結論을 얻었다. (1) Rabbit Anti-Trichomonas vaginalis servum에 對한 Trichomonas vaginalis의 抗原抗體反應에서 最大抗體價는 1:256으로 陽性이었으며, Trichomonas muris와 Trypanosoma cruzi의 境遇, 最大抗體價가 各各 1:4, 1:8에서 交又反應이 일어났고 Trichmonas tenax는 陰性이었다. Rabbit Anti-Trichomonas cruzi serum에서는 Trypanosoma cruzi만이 最大抗體價가 1:128로 陰性이었으며 其他 3種의 Trichomonas는 모두 陰性을 나타냈다. (2) Entameba histolytica(Ys-27)의 抗血淸에 對한 Entamoeba histolytica (Ys-27)의 抗原의 抗原抗體反應은 1:256까지 陰性이었으며, Acanthamoeba sp. 는 1:4에서 交又反應이 있어났으나 無視할 程度이고, Entamoeba muris의 境遇는 陰性이었다. Entamoeba histolytica (HK-9)의 抗血淸에서 Entamoeba histolytica (Ys-27)은 1:32까지 交又反應이 일어났고, Entamoeba muris, Acanthamoeba sp. 는 陰性이었다. (3) Toxoplasma gondii의 Rh strain 抗血淸에 對하여 Rh-strain 自體의 反應에서 1:1024의 最大抗體價를 보였으며, Beverley strain의 抗血淸에 對한 最大抗體價는 1:64로 比較的 높은 交又反應이 일어났다. (4) 根足蟲類와 鞭毛蟲類의 制動反應에서 Entamoeba histolytica (Ys-27)의 抗血淸에 對한 Entamoeba histolytica (Ys-27)의 制動率은 38%, Entamoeba histolytica (HK-9)의 抗血淸에서는 50%으로 모두 陽性이었으며, Trichomonas vaginalis 抗血淸에 對한 Trichomonas vaginalis, Trichomonas tenax의 制動率은 各各 90%, 70%이였고, Trypanosoma cruzi는 45%로 陰性反應으로 看做되었으나 Trypanosoma cruzi 抗血淸에 對한 Trypanosoma cruzi 自體의 反應에서는 90%로 陽性이었고 그 外는 모두 陰性이었다. 以上의 成績으로 보아 間接螢光抗體法은 病原性 原生動物의 抗原特異性을 識別하는데 좋은 長點을 가지고 있었으며 制動反應도 運動性 原生動物類 識別에 一次的 方法으로 推薦되어야 할 것이다. Antigenic comparison of several pathogenic protozoans belonging to class Flagellata, class Rhizopoda (Sarcordina) and class Sporozoa was carried out indirect fluorescent antibody test (IFAT) and immobilization reaction (IR). It was possible for IFAT technique to classify antigenic' specificity between several piotczoan species in the same class except the strains of Toxoplasma gondii, so that this technique is recommendable for further deteailed taxonomical approach. It was also regarded as the best subsidiary immunological tool for the antigenic differentiation of most protozoans with motility.

同一培地內에서의 「痢疾아메바」와 「腸트리코모나스」의 集團變動

鄭坪林,張在景,曺泳善,李定熙 최신의학사 1970 最新醫學 Vol.13 No.4

This study was designed to investigate whether two population groups of. E. lzistolytica and T. homi,Fis exist together in the same ecological environment or not. Two protozoan parasites were cultivated at once in modified diphasic medium (Faust & Russell, 1964), and checked the trends of population variance daily or two-days interval. Sodium and potassium contents, and pH levels in the media were respectively measured. The results are summarized as follows: 1. In the media in which Ea hislolytica and Ts honzinis were cultured simultaneously, the former annihilated on the third subculture and yet the latter showed continuous increase. 2. In the media in which the single species was cultured, both E. histolytica and T. honzinis multiplied increasingly, though the latter was more conspicuous. 3. In the mixed group without subculture, all the Ea histolytica diminished within 96 hours, while Ts hominis survived even until 144 hours. The performation of subculture for Ts hominis may be permitted in modified diphasic medium once a week. 4. In the single culture groups without subculture, E. histolytica disappeared within 144 hours, but Ts hominis survived up to 144 hours. 5. In the media containing Ts hominis the amounts of sodium and potassium increased to higher level at 144 hours to compare the value at 24 hours, whereas the amounts in the media containing Ea histolytica decreased gradually. As shown in above results, the mixei cultivation with E.histolylica and Ts hominis was unadvisable in modified diphasic medium due to more exceptional multiplication of T. hominis than E. histolytica.

Paratenic Host로서의 닭을 통한 폐흡충의 실험적 감염

안영겸,정평림 연세대학교 원주의과대학 1991 원주의대논문집 Vol.4 No.1

Giardia에 의한 環境汚染과 感染에 관한 疫學的 硏究

金碩燦,鄭坪林,李根泰,宋種述 忠南大學校 環境問題硏究所 1987 環境硏究 Vol.5 No.1

Giardia lamblia is a pathogenic flagellate causing intestinal disorders such as diarrhea, abdominal pain and malabsorption of nutrients. Giardia is mainly infected by the ingestion of contaminated foods per os. Craun(1979) has recently reported that mass infection of this flagellate through the contaminated water supply systems is one of public health hazards. Also, so-called traveller's diarrhea is sometimes caused by Giardia infection(CDC, U.S.A., 1971). However, a few epidemiological studies figuring out the mode of infection or control measures of Giardia infection has been done so far in Korea. The present study was aimed to know the prevalence of Giardia infection in several Korean populations, detectability of this flagellate in water systems and the viability of the cysts against sewages and disinfectants applying to drinking water. In the present study, 388 stool specimens from orphanage children in Chun-joo, Chung-joo, On-yang and Chun-an areas and 538 stool specimens from inhabitants in Woo-do, In-chon and Chun-joo were examined by formalin-ether concentration technique to detect out Giardia cysts. On the other hand, water samples from 14 sites of Han River and its tributaries were collected in May through July, 1984. Fifty liter of water sample in each sampling site was then filtered through water filtering system deviced by U.S. Environmental Protection Agency and the sediments rinsed out from the thread rolls, a part of water filtering system, were examined to detect out the Giardia cysts. In order to observe the viability of Giardia cysts in the sewage samples, the cysts were treated in it at 4℃ or 25℃ for 7 through 28days. For this purpose, the cysts were also exposed to various concentrations of disinfectants such as chlorine, iodine and ozone ges for proper time intervals. After treatment, the viability test of the Giardia cysts were carried out by method of Rice and Schaefer(1981) with minor modification. The results obtained in this study were as follows: 1) The detection rates of G. lamblia cysts in the stool specimens were 18.3% in orphans and 4.3% in general examinees. 2) The prevalences of Giardia infection were higher in the young age groups than in adults. The highest positive rate was 18.4% in the age group less than 10. 3) Of 14 water specimens sampled from Han River system and its tributaries around the Seoul area, the Giardia cysts were detected from 4 samples, and no cyst was found in the water supply systems. 4) The cysts treated in the sewage survived for 28days at 4℃ and for 13 days at 25℃. 5) The cysts were completely destroyed within 60minutes by exposure to 8mg/ℓ of residual chlorine at 4℃ and within 30minutes by exposure to the same concentration of chlorine at 25℃. 6) The cysts were all dead when exposed to 1mg/ℓ of iodine for 60 minutes at 4℃ or 25℃. 7) The cysts were destroyed after 10-minute exposure in 0.15mg to 0.25mg of residual ozone gas per liter. Summarizing the above results, it is considered that Giardia infection is regarded as a water-borne disease and the cysts are able to be controlled by the application with the disinfectants in the water supply systems.

三槽式便所를 設置한 濟州道內 一部落民의 寄生蟲感染率 推移

蘇鎭琸,李根泰,鄭坪林,李在興,李定熙,金英漢,洪淳億 최신의학사 1968 最新醫學 Vol.11 No.8

Three compartment stool tank system was established in March, 1965 to each of the houses . of Yongheungri, Cheju-do. The trend of parasites prevalence, faithfulness of the people to paractice the system and effectiveness of the system for destruction of ascaris eggs were examined. 1. Only 22 houses among 65 utilized the system for 3 years. The remained 43, gave up to practice; 10 because of the destruction of the cemented tank and 33 due to the shortage of. food stuffs, for hogs. In this island, the people have been raised hogs with human excrets. 2. The prevalence of ascaris infection in the village and in Sinwom-ri, a control village where the human feces were used as food stuffs for hog, were as follows: Yongheung(%) Sinwom(%) March 1965 before medication 52.0 43.0 Dec. 1965 after medication 15.1 21.2 Feb. 1968 22.7 30.8 3. Ascaris eggs in the 3rd compartment, were destroyed 94-100%, whereas the eggs in semi-formed feces in hog sty survived 94%.

「질트리코모나스」(Trichomonas vaginalis)의 주별(株別) 병원성에 따른 Isozyme 양상에 관한 실험적 연구

劉能和,明春玉,鄭坪林,李根泰 연세대학교 의과대학 1984 연세의대논문집 Vol.17 No.1

宿主體內에서의 犬蛔충, 犬鉤충 및 肝吸충의 ¹³¹I-RISA 吸收分布

金相俊,鄭坪林,李根泰,高昌俊 中央醫學社 1977 中央醫學 Vol.33 No.1

Most of the parasites in nemathelminthes and platyhelrninthes have highly developed reproductive organs as 'compared with their digestive organs, and deposit heavily protein-rich eggs. One female adult of Ascaris lumbricoides lays more than 200, 000 eggs per a day, a female of hookworm more than 20, 000 eggs and more than 4,000 eggs from an adult of leaf-like liver fluke with length of 1. 0 to I.5cm. It is considered that these eggs-depositing parasites may take nutrients not only through degenerated mouth part but also through any other absorption mechanism, especially for the compensation of protein loss due to egg deposition From this viewpoint, an autoradiographic study was carried out for investigating the intake mechanism of protein into the above-mentioned parasites. Toxocara canis and Ancylostonza caninum were infected to the dogs, and Clonorchis sinensis was infected to the rabbits for the present study. A serum albumin for radioisotope, 1311-RISA, was also injected intravenously into each host, which was sacrificed 48 hours after injection The parasites were removed from the sacrificed hosts, and then the quantitative distribution of radioactivity were counted by Well Type Scintillation Counter from the parasites. The results are as follows; 1. Toxocara canis The dog ascaris harboring freely in the intestinal lumen of the host takes protein from the host through the cuticle as well as the primitive mouth :_. Part. Absorption amount of, serum protein was 1.2m; Ci per gm. Of body weight of the parasite and the absorption rate .was. 0.6%. The results indicate that the parasites take protein mainly from the contents of intestinal' lumen regardless of direct absorption from the intestinal wall of the host. 2. Ancylostoma caninum Canine hookworm intakes serum protein from the host only with its mouth part. Absorption amount .bf serum protein was 100.8 mpCi per gm. Of body weight of a parasite and 50.4% of absorption rate. Absorption amount of serum protein was actually 85 times higher than that of Toxocara canis. It is showed that blood-sucking through the mouth part with 3 pairs of teeth, is enough for protein metabolism in the canine hookworm. 3. Clonorchis sinensis The liver fluke inhabiting in the bile duct of the host liver, intook serum protein of the host with the oral sucker and integument. Absorption amount of serum protein was 60.0 mpCi per gm. Of body weight of the worm. The absorption rate (30.0%) was 50 times higher than that of Toxocara canis. It is suggested that serum protein is intaken from bile juice and wall of the host bile duct through the integument as well as the oral sucker of the liver fluke.

구순열 및 구개열 환자에 대한 Toxoplasma항체의 혈청학적 연구

심영기,이세일,홍인표,정평림,장효죽 大韓成形外科學會 1993 Archives of Plastic Surgery Vol.20 No.1

The etiology of the cleft lip and palate can be explained with a multifarious theory. According to articles, there are some relationships between congenital toxoplasmosis and the cleft lip&palate, but the direct relationships is not obvious. Therefore, this research aims to find the difference of the serum toxoplasma antibody titer between 172 cleft patients who have been treated at National Medical Center and their mother group of 81 cases, healthy control group of 150 cases. The antibody titer was identified by the indirect fluorescent antibody test(>16)and the enzyme-linked immunosorbent assay(>0.3). The results are as follow : 1. By ELISA, the positive rate of the cleft patients(8.1%, 14cases)and that of the mother group(9.9%, 8cases)is higher than the healthy control group(2.7%, 4cases)(p<0.05). 2. By IFAT, the positive rate of the cleft patients(10.5%, 18cases)is higher than the healthy control group(5.3%, 8cases), but the difference is not significant(p>0.05). And the positive rate of the mother group(13.6%, 11cases)is higher than the healthy control group(5.3%, 8cases)(p<0.05). 3. By ELISA and IFAT, the positive rate of the mother group is higher than the cleft patient but the difference is not significant. 4. By ELISA and IFAT, the positive rate of the cleft patients according to the clincal group is not significant. 5. By ELISA and IFAT, the positive rate of the chronic disease group(17.3% & 27.7%)is higher than the healthy control group(2.7% & 5.3%)(p<0.05).