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Ok-Chul Chung,Hong-Gyu Kang,Tae-Woong Bae,Hyeon-Jin Sun,Jeong-Keun Choi,Pyung Ok Lim,Hyo-Yeon Lee 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
Daily shedding pattern and longevity of pollen are important consideration for the evaluation of gene flow of transgenic plants. During the day, the pollen shedding pattern of zoysiagrass was determined in the lawn by using a device to collect airborne pollen on a glass slide, resulting that the pollen grains were released predominantly between 7:00 and 9:00. The result was also supported by in vitro pollen germination test, which was performed with pollens collected from 1:00 through 24:00 at 1h interval. Influence of temperature and humidity on pollen longevity was determined by germinating pollen at 25°C after incubating them for 10, 30, 60, and 180 min under different temperatures and humidity with pollen of zoysiagrass that opened freshly at about 9:00. The result showed that pollen longevity of zoysiagrass was sensitive to change of temperature and humidity and longest under the temperture and humidity of 15-20°C and 80-99%, respectively. Under natural conditions with the same method as upper controlled conditions, was determined pollen longevity. Under sunny atmospheric conditions, pollen longevity decreased to 20% in 60 min, with a complete extinction in 120 min. Under cloudy atmospheric conditions, pollen remained viable up to 450 min, with about 20% longevity after 360 min. No significant difference was found between GM and non-GM plants in their pollen longevity.
A cyclic RGD-coated peptide nanoribbon as a selective intracellular nanocarrier
Lim, Yong-beom,Kwon, Oh-Joon,Lee, Eunji,Kim, Pyung-Hwan,Yun, Chae-Ok,Lee, Myongsoo Royal Society of Chemistry 2008 Organic & Biomolecular Chemistry Vol.6 No.11
<P>We have synthesized a peptide-based supramolecular building block consisting of a cyclic Arg-Gly-Asp (cRGD) peptide segment and a β-sheet-forming peptide segment. The block peptide was shown to self-assemble into a cRGD-coated nanoribbon structure, as revealed by circular dichroism (CD), dynamic light scattering (DLS), and transmission electron microscopy (TEM) studies. We have shown that this cRGD-coated nanoribbon can encapsulate hydrophobic guest molecules and deliver them into cells. Colocalization of the nanoribbon with LysoTracker and the selective intracellular delivery results suggests that the cRGD-coated nanoribbon is likely to be internalized into the cells through integrin receptors.</P> <P>Graphic Abstract</P><P>A β-ribbon coated with cyclic Arg-Gly-Asp (cRGD) can encapsulate hydrophobic guest molecules, and deliver them into cells selectively. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b802470g'> </P>
Lim, Pyung Ok,Kim, Yumi,Breeze, Emily,Koo, Ja Choon,Woo, Hye Ryun,Ryu, Jong Sang,Park, Don Ha,Beynon, Jim,Tabrett, Alex,Buchanan-Wollaston, Vicky,Nam, Hong Gil Blackwell Publishing Ltd 2007 The Plant journal Vol.52 No.6
<P>Summary</P><P>Leaf senescence is the final stage of leaf development and is finely regulated via a complex genetic regulatory network incorporating both developmental and environmental factors. In an effort to identify negative regulators of leaf senescence, we screened activation-tagged Arabidopsis lines for mutants that exhibit a delayed leaf senescence phenotype. One of the mutants (<I>ore7-1D</I>) showed a highly significant delay of leaf senescence in the heterozygous state, leading to at least a twofold increase in leaf longevity. The activated gene (<I>ORE7</I>/<I>ESC</I>) encoded a protein with an AT-hook DNA-binding motif; such proteins are known to co-regulate transcription of genes through modification of chromatin architecture. We showed that ORE7/ESC, in addition to binding to a plant AT-rich DNA fragment, could also modify the chromatin architecture, as illustrated by an altered distribution of a histone–GFP fusion protein in the nucleus of the mutant. Globally altered gene expression, shown by microarray analysis, also indicated that activation of <I>ORE7</I>/<I>ESC</I> results in a younger condition in the mutant leaves. We propose that ectopically expressed <I>ORE7</I>/<I>ESC</I> is negatively regulating leaf senescence and suggest that the resulting chromatin alteration may have a role in controlling leaf longevity. Interestingly, activation of <I>ORE7</I>/<I>ESC</I> also led to a highly extended post-harvest storage life.</P>
Potential Therapeutics Against Flaviviruses
Lim, Pyung-Ok,Lee, Tae-Hee,Chung, Kyung-Min The Korean Society for Microbiology 2012 Journal of Bacteriology and Virology Vol.42 No.2
Flaviviruses have been important human pathogens after emerging and resurging flavivirus diseases over the past decades. Although effective therapeutic agents are not yet commercially available for use in humans, significant progress has been made toward developing effective therapeutics and treatments. Several studies have shown that antibodies against the flaviviral E and NS1 proteins play a central role in prophylaxis and/or treatment of flavivirus infection through passive immunization. In addition, many anti-flavivirals, including interferons, oligonucleotide-based platforms, and small compounds, have been developed and evaluated for their antiviral effects. This review provides an overview of various approaches to the development of anti-flaviviral candidates and new insights that could improve our strategies for designing effective therapeutics against flaviviruses.
Auxin response factor 2 (ARF2) plays a major role in regulating auxin-mediated leaf longevity
Lim, Pyung Ok,Lee, In Chul,Kim, Junyoung,Kim, Hyo Jung,Ryu, Jong Sang,Woo, Hye Ryun,Nam, Hong Gil Oxford University Press 2010 Journal of experimental botany Vol.61 No.5
<P>Auxin regulates a variety of physiological and developmental processes in plants. Although auxin acts as a suppressor of leaf senescence, its exact role in this respect has not been clearly defined, aside from circumstantial evidence. It was found here that <I>ARF2</I> functions in the auxin-mediated control of <I>Arabidopsis</I> leaf longevity, as discovered by screening EMS mutant pools for a delayed leaf senescence phenotype. Two allelic mutations, <I>ore14-1</I> and <I>14-2</I>, caused a highly significant delay in all senescence parameters examined, including chlorophyll content, the photochemical efficiency of photosystem II, membrane ion leakage, and the expression of senescence-associated genes. A delay of senescence symptoms was also observed under various senescence-accelerating conditions, where detached leaves were treated with darkness, phytohormones, or oxidative stress. These results indicate that the gene defined by these mutations might be a key regulatory genetic component controlling functional leaf senescence. Map-based cloning of <I>ORE14</I> revealed that it encodes ARF2, a member of the auxin response factor (ARF) protein family, which modulates early auxin-induced gene expression in plants. The <I>ore14/arf2</I> mutation also conferred an increased sensitivity to exogenous auxin in hypocotyl growth inhibition, thereby demonstrating that ARF2 is a repressor of auxin signalling. Therefore, the <I>ore14/arf2</I> lesion appears to cause reduced repression of auxin signalling with increased auxin sensitivity, leading to delayed senescence. Altogether, our data suggest that ARF2 positively regulates leaf senescence in <I>Arabidopsis</I>.</P>
Kim, Pyung-Whan,Lim, Jong-Deuk,Lee, Seong-Hee,Oh, Kwi-Ok,Kim, Hyung-Seop The Official Publication of Korean Academy of Oral 1993 International Journal of Oral Biology Vol.17 No.2
The control of potentially periodontopathic microorganisms by host polymorphonuclear leukocytes (PMNs) is crucial to periodontal health. Function of PMNs is controlled by various proinflammatory cytokines, such as IL-1, IL-6, and TNF-α, MIP-1α and MIP-1β are members of intercrine or chemokine superfamily which show chemokinetic, pyrogenic, and other proinflammatory actions. We performed the in vitro assay for the antimicrobial action of human and rat peripheral PMNs and differentiated promyelocytic cell line HL60 cells by using S. aureus. MIP-1α enhanced antimicrobial action of human PMNs which was completely blocked by specific antibody pretreatment. On the contrary. MIP-1β suppressed human PMNs, antimicrobial action dose-dependently and MIP-1α antagonized suppressive antimicrobial action of MIP-1β. Above mentioned specific enhancing effect of MIP-1β on antimicrobial action of PMNs were almost identical to that of other source of PMNs, namely peripheral blood of rat. Differentiated HL60 cells showed absolutely higher antimicrobial action when compared with undifferentated cells. Antimicrobial action of differentiated HL60 cells was also increased by MIP-1α treatment.
Kim, In OK,Kim, In Chul,Kim, Sunmi,Kwon, Yeon Kyung,Han, Pyung-Lim,Jeon, Sang-Hak,Kim, Sang Hee Wiley Subscription Services, Inc., A Wiley Company 2005 Journal of neurobiology Vol.62 No.4
<P>Dorsoventral patterning of the Drosophila ventral neuroectoderm is established by the expression of three evolutionarily conserved homeodomain genes: ventral nervous system defective (vnd), intermediate neuroblasts defective (ind), and muscle segment homeobox (msh) in the medial, intermediate, and lateral columns of the ventral neuroectoderm, respectively. It was not clear whether extrinsic factor(s) from the CNS midline cells influence the initial dorsoventral patterning by controlling the expression of the dorsoventral patterning genes. We show here that the CNS midline cells, specified by single-minded (sim), are essential for maintaining expression of the dorsoventral patterning genes. Ectopic expression of sim in the ventral neuroectoderm during the blastoderm stage repressed expression of the three homeodomain genes in the ventral neuroectoderm. This indicates that the identity of the CNS midline cells is established by a series of repressions of the three homeodomain genes in the ventral neuroectoderm. Ectopic expression of sim in the ventral neuroectoderm during initial neurogenesis induced ectopic ind expression in the medial column in addition to that in the intermediate column via EGFR signaling between the ventral neuroectoderm and midline cells. In contrast, it repressed the expression of vnd and msh in the medial and lateral columns, respectively. Our findings demonstrate that the CNS midline cells provide extrinsic positional information via EGFR signaling that maintains the initial subdivision of the ventral neuroectoderm into three dorsoventral columns during initial neurogenesis. © 2004 Wiley Periodicals, Inc. J Neurobiol, 2005</P>