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( Sadan Dahal ),( Prakash Chaudhary ),( Yi-sook Jung ),( Jung-ae Kim ) 한국응용약물학회 2023 Biomolecules & Therapeutics(구 응용약물학회지) Vol.31 No.2
Prostate cancer is the fifth leading cause of cancer-related mortality in men, primarily because of treatment resistance, recurrence, and metastasis. In the present study, we investigated the role of paracrine interleukin-8 (IL-8) in the antagonistic expression of IL-8 and androgen receptor (AR), and the contribution of IL-8 to prostate cancer aggressiveness. In hormone-responsive LNCaP cells that do not express IL-8, recombinant IL-8 treatment significantly increased expressions of IL-8, CXC chemokine receptor 2 (CXCR2), matrix metalloproteinase (MMP)-2/9, Snail, and vimentin. IL-8 treatment significantly decreased AR and E-cadherin expression. IL-8-induced gene expression changes were suppressed by navarixin, a CXCR1/2 inhibitor, and gallein, a Gβγ inhibitor. In PC-3 androgen-refractory prostate cancer cells, IL-8 knockdown reduced expressions of CXCR2, MMP-2/9, Snail, and vimentin, and increased AR and E-cadherin expressions at the mRNA and protein levels. Co-culture with MEG-01 human megakaryocytic cells secreting high levels of IL-8 induced gene expression changes in both LNCaP and PC-3 cells, similar to those induced by IL-8 treatment. The altered gene expressions were accompanied by significant activation of transcription factor Snail in LNCaP and PC-3 cells. Treatment with the CXCR blocker navarixin inhibited the invasion of PC-3 cells but not LNCaP cells. However, invasion induced by MEG-01 was inhibited by navarixin in both LNCaP and PC-3 cells. The collective findings demonstrate that IL-8 enhances CXCR2 expression, which antagonistically regulates AR expression. More importantly, through changes in IL-8/CXCR2-regulated gene expression, IL-8 induces antiandrogen therapy resistance and epithelial-mesenchymal transition in prostate cancer.
Saurabh Badoni,Reeku Chaudhary,Ravi Shekhar,Shweta Badoni,Ekhlaque Ahmad,Rishi Pal Gangwar,Kashi Nath Tiwari,Rajendra Singh Rawat,Deepshikha,Jai Prakash Jaiswal 한국작물학회 2017 Journal of crop science and biotechnology Vol.20 No.5
Stripe rust of wheat caused by the fungal pathogen is a destructive foliar disease of wheat. Thus, it is crucial step to characterize the resistant germplasm for stripe rust in a diverse germplasm pool for their ultimate utilization in efficient crop rust resistance breeding. In the present study, we followed two pronged strategies involving integrated phenotypic and molecular characterization of 440 diverse wheat germplasm lines for rust resistance. The germplasm panel was extensively evaluated in field epiphytotic conditions during two consecutive years. After rigorous screening, 72 accessions were successfully revealed as resistant to moderately resistant to stripe rust. Subsequently, entries were then evaluated for their field agronomicperformances, considering prerequisites for serving as a donor germplasm,particularly for yield and 33 potential rust-resistant accessions were identified. Furthermore, to detect the sources of resistance, accessions were molecular characterized for potential race-specific resistance genes Yr5, Yr10,Yr15, and effective adult plant resistance (APR) gene Lr34/Yr18/pm38. We identified the 22 accessions possessing one or more single resistance genes and two accessions were observed with at least three of them. Moreover, Lr34/Yr18/pm38 was determined to confer resistance when observed along with any of the race-specific genes. Thus, the study not only provides proof of concept methodology to identify candidate resistant sources from large germplasm collections but simultaneouslyconfirmed the contribution of combining race-specific andnon-specific APR genes. The finding could further assist in the potential deployment of resistant genes directly into the stripe rust breeding program by involving marker-assisted approaches.
A comprehensive checklist of threatened plants of Meghalaya, Northeast India
Khrote-u Lasushe,Aabid Hussain Mir,Prem Prakash Singh,Kanhaiya Lal Chaudhary,Hiranjit Choudhury,Chaya Deori,Dilip Kumar Roy,Bikarma Singh,Krishna Upadhaya 국립중앙과학관 2022 Journal of Asia-Pacific Biodiversity Vol.15 No.3
In this era of rapid biodiversity decline, creating a checklist of threatened taxa is a prerequisite as it apprises the conservationists about the current status of species, thereby enabling the enforcement of necessary measures to prevent them from extinction. The present study was carried out to develop a comprehensive list of threatened species of Meghalaya using both the global and regional lists viz., In ternational Union for Conservation of Nature Red List, Red Data Book of Indian Plants and Conservation Assessment and Management Plan. The analysis revealed the presence of 385 plant taxa belonging to 274 genera and 108 families in the state under various threatened categories. The dominant life form con sisted of trees (40.26%), followed by herbs (35.84%), shrubs (13.25%), climbers (5.45%), epiphytes (4.94%) and parasites (0.26%). Fabaceae with 34 species was the largest family and Magnolia with 14 species was the dominant genera. The distribution of the threatened species showed that 24 species are exclusively endemic to Meghalaya and 70 species were restricted to Northeastern India, Indo-Burma or the Eastern Himalaya region. The present study has enabled the compilation of data on threatened plants of Meghalaya spread across literature with an update on their distributional area.