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        Assessment of insect diversity along an altitudinal gradient in Pinderi forests of Western Himalaya, India

        Prakash Chandra Joshi,KISHORE KUMAR,Manoj Arya 한국응용곤충학회 2008 Journal of Asia-Pacific Entomology Vol.11 No.1

        Insect diversity, richness and abundance were evaluated at different altitudes in three forest habitats in the Western Himalayas. The habitats studied were all situated between 2100 and 3500 m and included a site with no disturbances, a site with a moderate level of disturbance and a site with a very high level of disturbance. The species composition and diversity of insects varied at all the three study sites, which demonstrates the effect of altitude and disturbances, as well as the effects of other ecological and climatic parameters on insect populations. The site at lowest altitude, which contained a moderate level of disturbance, supported the highest number of species (108), whereas the site at the highest altitude, which contained the maximum level of disturbance, supported the lowest number of species (77). When all of the sites were considered, 122 species of insects belonging to 43 families and 8 orders were recorded. Lepidopterawas the most dominant insect order recorded, with 46 species being observed. This was followed by Hymenoptera (20), Coleoptera (18), Orthoptera (12), Hemiptera (10), Odonata (9), Diptera (5) and Dermeptera (2). The most abundant species were Vanessa cashmirensis Fru. (Lepidoptera: Nymphalidae), Pieris canidia indica Sparr. (Lepidoptera: Pieridae), Apis laboriosa Smith (Hymenoptera: Apidae), Anomala dimidiata Hope (Coleoptera: Scarabidae), Chorthipus sp. (Orthoptera: Acrididae), Crocothemis servilia servilia (Drury) (Odonata: Libellulidae) and Syrphus fulvifacies Brunetti (Diptera: Syrphidae). The site at the lowest altitude and the sites with the longest rainy seasons had the highest Shannon–Wiener Diversity. Insect diversity, richness and abundance were evaluated at different altitudes in three forest habitats in the Western Himalayas. The habitats studied were all situated between 2100 and 3500 m and included a site with no disturbances, a site with a moderate level of disturbance and a site with a very high level of disturbance. The species composition and diversity of insects varied at all the three study sites, which demonstrates the effect of altitude and disturbances, as well as the effects of other ecological and climatic parameters on insect populations. The site at lowest altitude, which contained a moderate level of disturbance, supported the highest number of species (108), whereas the site at the highest altitude, which contained the maximum level of disturbance, supported the lowest number of species (77). When all of the sites were considered, 122 species of insects belonging to 43 families and 8 orders were recorded. Lepidopterawas the most dominant insect order recorded, with 46 species being observed. This was followed by Hymenoptera (20), Coleoptera (18), Orthoptera (12), Hemiptera (10), Odonata (9), Diptera (5) and Dermeptera (2). The most abundant species were Vanessa cashmirensis Fru. (Lepidoptera: Nymphalidae), Pieris canidia indica Sparr. (Lepidoptera: Pieridae), Apis laboriosa Smith (Hymenoptera: Apidae), Anomala dimidiata Hope (Coleoptera: Scarabidae), Chorthipus sp. (Orthoptera: Acrididae), Crocothemis servilia servilia (Drury) (Odonata: Libellulidae) and Syrphus fulvifacies Brunetti (Diptera: Syrphidae). The site at the lowest altitude and the sites with the longest rainy seasons had the highest Shannon–Wiener Diversity.

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        Evaluating the DNA barcodes for identification of butterflies from Western Himalayas, Uttarakhand, India

        Singh Vinaya Kumar,Joshi Prakash Chandra,Gupta Sandeep Kumar 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.2

        DNA barcodes in species tagging have become a popular tool for taking inventories of species from different groups worldwide. The present study aimed to generate DNA barcodes of butterfly species from the Western Himalayas in Uttarakhand, India. The Indian Western Himalayan region (IWHR) has been explored to a limited extent about butterfly species’ diversity. However, the IWHR is prone to environmental change, and slight variations in climatic conditions can influence species diversity and change butterflies’ range. The mitochondrial cytochrome c oxidase I (COI) gene was first used to generate the DNA barcode for butterflies from this region on a broad scale. 28 morphologically identified species, consisting of 102 sequences, were finally grouped into 26 species, with only two species showing ambiguity in species identification. These species had < 3% sequence variations from their neighboring relatives, suggesting cryptic species diversity. Generated sequences were also compared with the GenBank data of conspecific geographical locations, which showed intraspecies variation ranging from 1.3% to 7.3%. It was also noted that butterfly species have both intra and interspecies sequence divergence. In the phylogenetic-based species identification, a total of 28 species belonging to 4 families of butterflies were successfully discriminated, and two species at the genus level, which had high intra-specific divergence (0.025), were considered. However, the high intra-species sequence divergence observed may represent the presence of hidden species.

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