Moisture Content Measurement on Peanuts by using Time Domain Reflectometry

( Sheng Peng Niu ),( Tzu Hua Chen ),( Tse Min Chen ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1

The Moisture content detection of non-homogeneous materials has always been a challenge, especially for agricultural products with time varied characteristics and specific compositions. For in-shelled peanuts, this research developed a system with which is able to detect moisture content promptly. Peanuts with high quality have to keep dry in all kinds of phases such as harvesting, storage, and processing. To prevent mold from breeding and the spread of aflatoxin, which will influence the value and quality severely, the moisture content of peanuts is a key parameter to be monitored and controlled. Therefore, a highly efficient device or mechanism will be required. However, there is still no any devices with enough accuracy and promptness to detect moisture content for peanuts yet. This study employed TDR (Time Domain Reflectometry) technology to challenge this mission. Comparing with the results of traditional IR measurement and standard oven, which helps to set up a calibration curve, this research validates the proposed method to detect the moisture content of in-shell peanut finally.

Mobile Social Helping Platform of LBS

Chen Yuefeng,Li Bingquan,Gao sheng,Peng Linxi 보안공학연구지원센터 2015 International Journal of Smart Home Vol.9 No.1

Mobile Internet grows with demands of location information of mobile users . In order to overcome the limitation of sociality, we develop Mobile social helping platform (MSH) of LBS, which realize the sharing of sociality. First, we analyze the requirement of MSHP platform, then design its architecture and database, and finally discuss the key technologies of MSHP. The platform makes use of open source technology, implements and optimizes LBS service. The simulation results show the system has good portability and maintainability, which is easy to be commercialized.

Wind tunnel study on fluctuating internal pressure of open building induced by tangential flow

Sheng Chen,Peng Huang,Richard G. J. Flay 한국풍공학회 2021 Wind and Structures, An International Journal (WAS Vol.32 No.2

This paper describes a wind tunnel test on a 1:25 scale model of TTU building with several adjustable openings in order to comprehensively study the characteristics of fluctuating internal pressures, especially the phenomenon of the increase in fluctuating internal pressures induced by tangential flow over building openings and the mechanism causing that. The effects of several factors, such as wind angle, turbulence intensity, opening location, opening size, opening shape and background porosity on the fluctuating internal pressures at oblique wind angles are also described. It has been found that there is a large increase in the fluctuating internal pressures at certain oblique wind angles (typically around 60° to 80°). These fluctuations are greater than those produced by the flow normal to the opening when the turbulence intensity is low. It is demonstrated that the internal pressure resonances induced by the external pressure fluctuations emanating from flapping shear layers on the sidewall downstream of the windward corner are responsible for the increase in the fluctuating internal pressures. Furthermore, the test results show that apart from the opening shape, all the other factors influence the fluctuating internal pressures and the internal pressure resonances at oblique wind angles to varying degrees.

Molecular Recognition Enables Nanosubstrate-Mediated Delivery of Gene-Encapsulated Nanoparticles with High Efficiency

Peng, Jinliang,Garcia, Mitch André,Choi, Jin-sil,Zhao, Libo,Chen, Kuan-Ju,Bernstein, James R.,Peyda, Parham,Hsiao, Yu-Sheng,Liu, Katherine W.,Lin, Wei-Yu,Pyle, April D.,Wang, Hao,Hou, Shuang,Tse American Chemical Society 2014 ACS NANO Vol.8 No.5

<P/><P>Substrate-mediated gene delivery is a promising method due to its unique ability to preconcentrate exogenous genes onto designated substrates. However, many challenges remain to enable continuous and multiround delivery of the gene using the same substrates without depositing payloads and immobilizing cells in each round of delivery. Herein we introduce a gene delivery system, nanosubstrate-mediated delivery (NSMD) platform, based on two functional components with nanoscale features, including (1) DNA⊂SNPs, supramolecular nanoparticle (SNP) vectors for gene encapsulation, and (2) Ad-SiNWS, adamantane (Ad)-grafted silicon nanowire substrates. The multivalent molecular recognition between the Ad motifs on Ad-SiNWS and the β-cyclodextrin (CD) motifs on DNA⊂SNPs leads to dynamic assembly and local enrichment of DNA⊂SNPs from the surrounding medium onto Ad-SiNWS. Subsequently, once cells settled on the substrate, DNA⊂SNPs enriched on Ad-SiNWS were introduced through the cell membranes by intimate contact with individual nanowires on Ad-SiNWS, resulting in a highly efficient delivery of exogenous genes. Most importantly, sequential delivery of multiple batches of exogenous genes on the same batch cells settled on Ad-SiNWS was realized by sequential additions of the corresponding DNA⊂SNPs with equivalent efficiency. Moreover, using the NSMD platform <I>in vivo</I>, cells recruited on subcutaneously transplanted Ad-SiNWS were also efficiently transfected with exogenous genes loaded into SNPs, validating the <I>in vivo</I> feasibility of this system. We believe that this nanosubstrate-mediated delivery platform will provide a superior system for <I>in vitro</I> and <I>in vivo</I> gene delivery and can be further used for the encapsulation and delivery of other biomolecules.</P>

Function and Dysfunction of Human Sinoatrial Node

정보영,Peng-Sheng Chen 대한심장학회 2015 Korean Circulation Journal Vol.45 No.3

Sinoatrial node (SAN) automaticity is jointly regulated by a voltage (cyclic activation and deactivation of membrane ion channels) and Ca2+ clocks (rhythmic spontaneous sarcoplasmic reticulum Ca2+ release). Using optical mapping in Langendorff-perfused canine right atrium, we previously demonstrated that the β-adrenergic stimulation pushes the leading pacemaker to the superior SAN, which has the fastest activation rate and the most robust late diastolic intracellular calcium (Cai) elevation. Dysfunction of the superior SAN is commonly observed in animal models of heart failure and atrial fibrillation (AF), which are known to be associated with abnormal SAN automaticity. Using the 3D electroanatomic mapping techniques, we demonstrated that superior SAN served as the earliest atrial activation site (EAS) during sympathetic stimulation in healthy humans. In contrast, unresponsiveness of superior SAN to sympathetic stimulation was a characteristic finding in patients with AF and SAN dysfunction, and the 3D electroanatomic mapping technique had better diagnostic sensitivity than corrected SAN recovery time testing. However, both tests have significant limitations in detecting patients with symptomatic sick sinus syndrome. Recently, we reported that the location of the EAS can be predicted by the amplitudes of P-wave in the inferior leads. The inferior P-wave amplitudes can also be used to assess the superior SAN responsiveness to sympathetic stimulation. Inverted or isoelectric P-waves at baseline that fail to normalize during isoproterenol infusion suggest SAN dysfunction. P-wave morphology analyses may be helpful in determining the SAN function in patients at risk of symptomatic sick sinus syndrome.

Directed Evolution of Beta-galactosidase from Escherichia coil into Beta-glucuronidase

( Ai Sheng Xiong ),( Ri He Peng ),( Jing Zhuang ),( Jin Ge Liu ),( Fang Xu ),( Bin Cai ),( Zhao Kui Guo ),( Yu Shan Qiao ),( Jian Min Chen ),( Zhen Zhang ),( Quan Hong Yao ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.3

Bayesian-theory-based Fast CU Size and Mode Decision Algorithm for 3D-HEVC Depth Video Inter-coding

( Fen Chen ),( Sheng Liu ),( Zongju Peng ),( Qingqing Hu ),( Gangyi Jiang ),( Mei Yu ) 한국인터넷정보학회 2018 KSII Transactions on Internet and Information Syst Vol.12 No.4

Multi-view video plus depth (MVD) is a mainstream format of 3D scene representation in free viewpoint video systems. The advanced 3D extension of the high efficiency video coding (3D-HEVC) standard introduces new prediction tools to improve the coding performance of depth video. However, the depth video in 3D-HEVC is time consuming. To reduce the complexity of the depth video inter coding, we propose a fast coding unit (CU) size and mode decision algorithm. First, an off-line trained Bayesian model is built which the feature vector contains the depth levels of the corresponding spatial, temporal, and inter-component (texture-depth) neighboring largest CUs (LCUs). Then, the model is used to predict the depth level of the current LCU, and terminate the CU recursive splitting process. Finally, the CU mode search process is early terminated by making use of the mode correlation of spatial, inter-component (texture-depth), and inter-view neighboring CUs. Compared to the 3D-HEVC reference software HTM-10.0, the proposed algorithm reduces the encoding time of depth video and the total encoding time by 65.03% and 41.04% on average, respectively, with negligible quality degradation of the synthesized virtual view.

Strength and Deformation Characteristics of Calcareous Sands Improved by PFA

Qing-Sheng Chen,Wan Peng,Gao-LiangTao,Sanjay Nimbalkar 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.1

Calcareous sand is widely distributed in the islands of the South China Sea, which could be promisingly used as the construction materials. However, particle breakage commonly occurs in calcareous sands, which may significantly influence their mechanical characteristics. To address these issues, an eco-friendly agent, i.e., polyurethane foam adhesive (PFA) is proposed to improve the engineering properties of calcareous sands, compared to the commonly used alkaline stabilizing agents (e.g., lime, cement). The objective of this work is to examine the effectiveness of using PFA in improving the strength-deformation properties of calcareous sand. A series of laboratory tests including direct shear tests, unconfined compression tests, and oedometer tests were performed on the calcareous sands improved by PFA. In addition, A scanning electron microscope (SEM) was conducted to reveal microstructural analysis of using PFA for calcareous sand. The experimental results provided insights into the shear strength, deformation modulus, as well as the micro-structural characteristics of improved calcareous sands with various PFA contents and particle size distributions.

Improvement of the Thermostability of Xylanase from Thermobacillus composti through Site-Directed Mutagenesis

( Yong-sheng Tian ),( Jing Xu ),( Lei-chen ),( Xiao-yan Fu ),( Ri-he Peng ),( Quan-hong Yao ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.10

Thermostability is an important property of xylanase because high temperature is required for its applications, such as wood pulp bleaching, baking, and animal feedstuff processing. In this study, XynB from Thermobacillus composti, a moderately thermophilic gram-negative bacterium, was modified via site-directed mutagenesis (based on its 3D structure) to obtain thermostable xylanase, and the properties of this enzyme were analyzed. Results revealed that the half-life of xylanase at 65°C increased from 10 to 50 min after a disulfide bridge was introduced between the α-helix and its adjacent β-sheet at S98 and N145. Further mutation at the side of A153E named XynB-CE in the C-terminal of this α-helix enhanced the half-life of xylanase for 60 min at 65°C. Therefore, the mutant may be utilized for industrial applications.

Stochastic intelligent GA controller design for active TMD shear building

Z.Y. Chen,Sheng-Hsiang Peng,Ruei-Yuan Wang,Yahui Meng,Qiuli Fu,Timothy Chen 국제구조공학회 2022 Structural Engineering and Mechanics, An Int'l Jou Vol.81 No.1

The problem of optimal stochastic GA control of the system with uncertain parameters and unsure noise covariates is studied. First, without knowing the explicit form of the dynamic system, the open-loop determinism problem with path optimization is solved. Next, Gaussian linear quadratic controllers (LQG) are designed for linear systems that depend on the nominal path. A robust genetic neural network (NN) fuzzy controller is synthesized, which consists of a Kalman filter and an optimal controller to assure the asymptotic stability of the discrete control system. A simulation is performed to prove the suitability and performance of the recommended algorithm. The results indicated that the recommended method is a feasible method to improve the performance of active tuned mass damper (ATMD) shear buildings under random earthquake disturbances.