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Peiying Hao,Yan Ma,Yalin Feng,Chaofeng Lu,Xiaoping Yu 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.1
CDK1 is a kind of cyclin-dependent kinases (CDKs) involved in diverse biological processes besides cell cycleregulation in various species. However, little is known about CDK1 in Nilaparvata lugens Stål (brown planthopper,BPH). In this study, the full-length cDNA of NlCDK1, a CDK1 gene in BPH, was obtained using rapidamplificationof cDNA ends (RACE) technique. RT-qPCR revealed that the expression of NlCDK1 was relativelylow and stable at nymph stages from 1st star to 5th star, but increased to higher levels in gravid females 3 day to5 day post-eclosion. Feeding-based RNA interference (RNAi) with 0.5 μg/μL dsNlCDK1 achieved an efficientknockdown of target gene expression in females after being fed with dsRNA for 6 days, and the mRNA level ofNlCDK1 decreased to 19.9% in comparison with that of the dsGFP control. As a result, RNA inerference withdsNlCDK1 caused a significant decrease of the BPH survival rate to 28.0% of that for the control. RNAi of NlCDK1also suppressed the ovarian development and significantly affected the fecundity of the females, leading to a70.2% reduction to the number of the offspring. These data suggest that NlCDK1 is required for the ovariandevelopment and survival of the brown planthopper.
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Chunping Yu,Yi Zhang,Ning Wang,Wensu Wei,Ke Cao,Qun Zhang,Peiying Ma,Dan Xie,Pei Wu,Biao Liu,Jiahao Liu,Wei Xiang,Xing Hu,Xuewen Liu,Jianfei Xie,Jin Tang,Zhi Long,Long Wang,Hongliang Zeng,Jianye Liu 한국생체재료학회 2022 생체재료학회지 Vol.26 No.1
Background: Circular RNAs (circRNAs) have important functions in many fields of cancer biology. In particular, we previously reported that the oncogenic circRNA, circPRMT5, has a major role in bladder cancer progression. Therapy based on circRNAs have good prospects as anticancer strategies. While anti-circRNAs are emerging as therapeutics, the specific in vivo delivery of anti-circRNAs into cancer cells has not been reported and remains challenging. Methods: Synthesized chrysotile nanotubes (SCNTs) with a relatively uniform length (~ 200 nm) have been designed to deliver an siRNA against the oncogenic circPRMT5 (si-circPRMT5) inhibit circPRMT5. In addition, the antitumor effects and safety evaluation of SCNTs/si-circPRMT5 was assessed with a series of in vitro and in vivo assays. Results: The results showed that SCNTs/si-circPRMT5 nanomaterials prolong si-circPRMT5’s half-life in circulation, enhance its specific uptake by tumor cells, and maximize the silencing efficiency of circPRMT5. In vitro, SCNTs encapsulating si-circPRMT5 could inhibit bladder cancer cell growth and progression. In vivo, SCNTs/si-circPRMT5 inhibited growth and metastasis in three bladder tumor models (a subcutaneous model, a tail vein injection lung metastatic model, and an in situ model) without obvious toxicities. Mechanistic study showed that SCNTs/sicircPRMT5 regulated the miR-30c/SNAIL1/E-adherin axis, inhibiting bladder cancer growth and progression. Conclusion: The results highlight the potential therapeutic utility of SCNTs/si-circPRMT5 to deliver si-circPRMT5 to treat bladder cancer.
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