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        NlCDK1 gene is required for the ovarian development and survival of the brown planthopper, Nilaparvata lugens Stål, as revealed by RNA interference

        Peiying Hao,Yan Ma,Yalin Feng,Chaofeng Lu,Xiaoping Yu 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.1

        CDK1 is a kind of cyclin-dependent kinases (CDKs) involved in diverse biological processes besides cell cycleregulation in various species. However, little is known about CDK1 in Nilaparvata lugens Stål (brown planthopper,BPH). In this study, the full-length cDNA of NlCDK1, a CDK1 gene in BPH, was obtained using rapidamplificationof cDNA ends (RACE) technique. RT-qPCR revealed that the expression of NlCDK1 was relativelylow and stable at nymph stages from 1st star to 5th star, but increased to higher levels in gravid females 3 day to5 day post-eclosion. Feeding-based RNA interference (RNAi) with 0.5 μg/μL dsNlCDK1 achieved an efficientknockdown of target gene expression in females after being fed with dsRNA for 6 days, and the mRNA level ofNlCDK1 decreased to 19.9% in comparison with that of the dsGFP control. As a result, RNA inerference withdsNlCDK1 caused a significant decrease of the BPH survival rate to 28.0% of that for the control. RNAi of NlCDK1also suppressed the ovarian development and significantly affected the fecundity of the females, leading to a70.2% reduction to the number of the offspring. These data suggest that NlCDK1 is required for the ovariandevelopment and survival of the brown planthopper.

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