Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

Park*, Eui‐,Chul,Yoon, Jong‐,Bok,Seong*, Jin‐,Sil,Choi, Kyoung‐,Soo,Kong, Eung‐,Sik,Kim, Yun‐,Jeong,Park, Young‐,Mee,Park, Eun‐,Mi Taylor Francis 2006 Preparative biochemistry & biotechnology Vol.36 No.1

<P>Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.</P>

Elevated TRAF2/6 expression in Parkinson's disease is caused by the loss of Parkin E3 ligase activity.

Chung, Ji-Yun,Park, Hee Ra,Lee, Su-Jin,Lee, Sun-Hye,Kim, Jin Sik,Jung, Youn-Sang,Hwang, Sang Hyun,Ha, Nam-Chul,Seol, Won-Gi,Lee, Jaewon,Park, Bum-Joon United States and Canadian Academy of Pathology [e 2013 Laboratory investigation Vol.93 No.6

<P>Parkinson's disease (PD) is the second leading neurodegenerative disease, and is known to be induced by environmental factors or genetic mutations. Among the verified genetic mutations of PD, Parkin, isolated from the PARK2 locus, shows an autosomal recessive inheritance pattern and is known to be an E3 ligase. However, the physiological target of Parkin and the molecular mechanism of Parkin-deficiency-induced PD have not been clearly demonstrated until now. It has recently been proposed that inflammation, suggesting as a causal factor for PD, is enhanced by Parkin deficiency. Thus, we examined the relationship between inflammation-related factors and Parkin. Here, we provide the evidence that Parkin suppresses inflammation and cytokine-induced cell death by promoting the proteasomal degradation of TRAF2/6 (TNF-α receptor-associated factor 2/6). Overexpression of Parkin can reduce the half-lives of TRAF2 and TRAF6, whereas si-Parkin can extend them. However, mutant Parkins did not alter the expression of TRAF2/6. Thus, loss of Parkin enhances sensitivity to TNF-α- or IL-1β-induced JNK activation and NF-κB activation. Indeed, si-Parkin-induced apoptosis is suppressed by the knockdown of TRAF6 or TRAF2. We also observed elevated expression levels of TRAF6 and a reduction of IκB in an 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced PD mouse model. Moreover, elevated expression levels or aggregation of TRAF6 were detected in approximately half of the human PD tissues (7/15 cases) and 2 cases, respectively. In addition, TRAF6 and Parkin expression levels show a reverse relationship in human PD tissues. Our results strongly suggest that the reduction of Parkin or overexpression of TRAF2/6 by chronic inflammation would be the reason for occurrence of PD.</P>

응급센터에 내원한 만성 폐쇄성 폐질환 환자에 대한 고찰

박승회,백광제,홍윤식 대한응급의학회 1993 대한응급의학회지 Vol.4 No.2

Chronic Obstrcitive Pulmonary Diseases(COPDs) produce significant morbvidity and mortality in human lives. COPD is a progressive disease that coexists with emphysema and chronic brochitis. The critical episodes depend on inciting or aggravating factors which can be classified into 3 ways. First, disease related aggravation. Second, Patient related aggravation. Third, Physician related(latrogenic). The authors reviewed 102 COPD patients who came to Korea University Hospital Emergency Medical center from March of 1992 to February of 1993. 1) Males were more frequently affected than females, and male to female ratio was 2.64 : 1 2) The average age of the patients were 62.8 years old. 3) The average number of hospital admission was 2.5 times and one patient was admitted 9 times for the same disease. 4) On an average, the patients had dyspneic symptoms for 15.6 years and the symptoms had developed since they were 47.8 years old. 5) The ratio between smokers and nonsmokers were3 2.1 : 1, the smokers had smoked for 36.3 years and the average amount of smoking was 1.5 pack per day. 6) After admission, sputum culture and sensitiveity test were done and the results were as follows : nonspceific organums 47 cases(46.0%), streptococcus 23 cases(22.5%), Pseudomonas 15 cases(14.7%), Hemophilus influenza 7 cases(6.8%), Klebsiella 5 cases(4.9%), Actinomycosis 3 cases (2.9%), Staphylococcus 2 cases(1.9%) 7) Aggravation factors for dyspnea were as follows : Upper Respiratory Tract Infection 40 cases(39.2%), Smoking 2.5 cases(24.5%), Pneumothorax 11 cases(10.8%), Pneumonia 7 cases(6.8%), Unknown origin 19 cases(18.6\%) 8) Prognosis after hospital admission were classified into 6 groups : 1. completely recovered 2. almost recovered 3. no improvement 4. hopeless discharge 5. expired within 48 hrs. 6. expired after 48hrs. The results were as follows : 87.3%(89 cases) discharged in almost recovered stated state, 1.9%(2 cases) discharged with no improvement, 4.9%(5 cases) were hoplessly discharged, 2.9%(3 cases) expired within 48 hrs, 2.9%(3 cases) expired after 48 hrs.

Simulated resin root canal에서 엔진 구동형 Ni-Ti 파일로 근관성형 후 Buchanan plugger의 근관 내 삽입깊이에 대한 비교 연구

박연식,김동준,황윤찬,황인남,오원만 大韓齒科保存學會 2006 Restorative Dentistry & Endodontics Vol.31 No.2

본 연구는 레진 블록에서 엔진구동형 Ni-Ti 기구를 이용하여 근관성형 후 Buchanan plugger의 삽입 깊이에 대한 비교를 통해 임상적 상황에서 Ni-Ti 파일로 근관 성형 후 Continuous wave of condensation technique을 이용한 근관 충전시 적절한 크기의 Buchanan plugger 선택에 지침을 제공하기 위해 시행되었다. 만곡근관과 직선근관을 가진 레진 블록에서 ProTaper와 ProFile을 이용하여 근관 성형을 하였다. 성형 후 근관에 Buchanan plugger 가 근관벽과 처음으로 접촉하는 부위까지 삽입한 후 그 상태에서 스태너를 이용하여 이미지를 얻었다. 이미지 분석 프로그램을 이용해 근첨에서 plugger 첨부까지의 거리를 얻었다. 각 기구에 대한 plugger 삽입깊이에 대한 유의성을 검정하기 위해 one-way ANOVA를 시행하였고, 사후검정을 위해 Scheffe's test를 시행하였다. 본 연구의 결과 만곡되고 좁읍 근관에서, ProTaper F2와 F3로 마무리 성형한 근관에서 F. FM 크기의 plugger를 사용할 경우 Continuous wave of condensation technique으로 적절한 근관충전이 가능함을 시사하였다. This study was conducted to evaluate the insertion depth of Buchanan plugger after shaping by various Ni-Ti rotary files. It was conducted to determine which size of plugger are appropriate, when root canals are shaped with Ni-Ti rotary files and obturated by Continuous wave of condensation technique. Two type of eighty simulated resin blocks were used : J-shaped and straight shaped canal. The simulated canals were instrumented by ProTaper and ProFile. Buchanan pluggers were inserted into the canal, and then the image was recorded to scanner. The distance from the apex of the canal to the plugger tip was measured by image analysis program. Data were analyzed by one-way ANOVA followed by Scheffe's test. The results were as follows 1. In straight canal finished up to ProTaper F2 and F3 file, F and FM pluggers were inserted more than 5 ㎜ short of working length. 2. In J-shaped canal finished up to ProTaper F2 file, F pluggers were inserted more than 5 ㎜ short of working length. Finished up to ProTaper F3 file, F and FM pluggers were inserted more than 5 ㎜ short of working length. 3. In straight and J-shaped canal finished up to ProFile . 06/#20 and ,06/#25, any of Buchanan plugger could not be inserted more than 5 ㎜ short of working lenghth. These results suggest that canals shaped by ProTaper could be obturated by Continuous wave of condensation technique with F and FM size Buchanan plugger.

뇌졸중 환자에서 아포지단백 E의 유전자형에따른 혈중 지질과 예후

김연희,박성희,신용일,서정환,김달식 大韓再活醫學會 2000 Annals of Rehabilitation Medicine Vol.24 No.4

지문 정합에 관한 연구

朴一男,金栢起,郭允植,李大寧 慶熙大學校 1991 論文集 Vol.20 No.-

In this paper, a fingerprint recognition system for identification and classification of fingerprint is described. To develop an efficient fingerprint identification system, modified algorithms are proposed in this thesis. The recognition algorithm consists of preprocessing(acquisition, binarization, thinning, restoration), classification and matching. Experimental thinning show that identification rate is about 91% for 80 file fingerprints and classification rate is more than 98% for 80 input fingerprints.

돼지 신장세포(PK15)의 이종이식 거부반응에 대한 재조합 IL-18의 영향

최연실,김은미,김영관,박상연,심정현,윤도영,강형식,명평근 충남대학교 생물공학연구소 2004 생물공학연구지 Vol.10 No.1

Various cytokines including IL-1β, IL-2, IL-4, IL-10, IL-12p40, IFN-γ, and TNF-α have been recently reported to play important roles in both host and donor cell activations during xenotransplantation. Interleukin (IL)-18, a proinflammatory cytokine, can induce IL-4 and IL-13 production in T cells, NK cells, B cells, mast cells, and basophils. IL-18 has the capacity to stimulate innate immunity and both Th1-and Th2- mediated responses, but little is known to involve in the xenograft rejection. In order to investigate the role of IL-18 in xenograft rejection, we transplanted the pig kidney (PK15) cells to C57BL/6 mouse with or without intraperitoneal injection of mouse recombinatant IL-18. It was analyzed the population of T cell, B cell and NK cell in the mice transplanted with PK 15 cells and recombinant IL-18 by flow cytometry. We found that splenic CD3+ T cells were increased in mice injected PK15 cells with recombinant IL-18. These results suggest that recombinant IL-18 is critical in the xenograft rejection by increasing T cell population of the recipients.

內部會計管理制度의 導入에 관한 硏究

황윤식,김석현,박정숙 東亞大學校 經營問題硏究所 2006 經營論叢 Vol.27 No.-

The United States Government established Sarbanes- Oxley Act in July 2002 because of the accounting fraud of Enron and Worldcom. The PCAOB(Public Company Accounting Oversight Board) published Auditing Standard No. 2 covering the auditor' s reporting on the ICFR(Intemal Control over Financial Reporting) in March 2004. These regulations were established on the basis of COSO framework. The accounting reform regulations have been introduced for accounting transparency in the aftermath of the foreign exchange crisis in late 1997 in Korea. The Korean Government established the Management’s Assessment Standards for the ICFR and the Accountant’s Review and Reporting Standards for the ICFR in June 2005. The purpose of this study is to find problems and provide future directions to improve the ICFR in Korea by making a comparison between the United State ICFR and the Korea ICFR. And these results of this study are expected to provide useful information regarding the introduction of ICFR in Korea.

돼지 신장세포(PK15)의 이종이식 거부반응에 대한 재조합 IL-18의 영향

최연실,김은미,김영관,박상연,심정현,윤도영,강형식,명평근 충남대학교 형질전환복제돼지연구센터 2004 논문집 Vol. No.8

Various cytokines including IL-1β, IL-2, IL-4, IL-10, IL-12p40, IFN-r, and TNF-α have been recently reported to play important roles in both host and donor cell activations during xenotransplantation. Interleukin (IL)-18, a proinflammatory cytokine, can induce IL-4 and IL-13 production in T cells, NK cells, B cells, mast cells, and basophils. IL-18 has the capacity to stimulate innate immunity and both Th1-and Th2- mediated responses, but little is known to involve in the xenograft rejection. In order to investigate the role of IL-18 in xenograft rejection, we transplanted the pig kidney (PK15) cells to C57BL/6 mouse with or without intraperitoneal injection of mouse recombinatant IL-18, It was analyzed the population of T cell, B cell and NK cell in the mice transplanted with PK 15 cells and recombinant IL-18 by flow cytometry. We found that splenic CD3+ T cells were increased in mice injected PKl5 cells with recombinant IL-18. These results suggest that recombinant IL-18 is critical in the xenograft rejection by increasing T cell population of the recipients.

머리염색이 인체에 미치는 영향

윤형식,황성호,이현륭,김수호,박연석,권낙현,정호진,김동훈,노현주,홍성호,박병찬,이관,정해관 東國大學校醫學硏究所 2002 東國醫學 Vol.9 No.1

일상생활에서 모발염색은 흔히 접할 수 있는 미용의 한 종류로 특히 젊은층을 중심으로 폭발적으로 유행하고 있다. 염색을 위항 사용하는 약제는 표백제와 발색제 등 각종 화학약품이 사용되고 있으나 이로 인한 건강장해에 대한 연구는 그리 많지 않다. 저자들은 염색이 인체의 모발건강에 미치는 영향을 파악하기 위하여 염색과 관련된 주관적 증상과 모발의 변화에 대한 실험적 연구를 시행하였다. 동국대학교 경주 캠퍼스 재학생 80명을 대상으로 설문조사를 시행하여 염색 유 ·무 및 염색 후에 경험한 증상에 대해 설문 조사하였고, 의과대학 재학생 46명을 대상으로 피부 반응 테스트를 실시하였다. 또한 염색 전후의 모발 탄성도를 측정하였고 모발의 상태를 파악하기 위해 전자 현미경검사를 실시하였다. 설문조사 결과 염색 전에 비하여 염색 후 안구혼탁, 안구건조, 시력저하, 발진 및 접촉성 피부염, 모발손상, 모근손상 등의 증상을 더 많이 경험한다고 호소하였다(p<0.05). 모발손상과 모근손상은 헤어드라이어 사용 빈도에 따라 증가하는 것으로 조사되었다(p<0.05). 피부반응검사에서 가려움증이 가장 많은 증상이었으며 이는 여성보다는 남성에서 높은 것으로 조사되었다. 염색 전후의 모발장력은 염색 전 134.5±10.37g, 염색 128.0±30.69g, 염색 이틀 후 112.5±19.69g으로 나타났다. 염색 전후의 모발의 전자현미경 케라틴 층이 현저히 감소하고 모발이 가늘어지는 차이를 보였다. 염색은 모발손상, 모발 케라틴 손상 및 모근 손상, 발진 및 접촉성 피부반응, 안구혼탁, 안구건조, 시력 저하를 유발한다. 따라서 염색약으로 인한 손상에 대한 주의와 예방이 필요하다고 생각한다. 예방대책으로 염색 전 피부테스트를 통한 적합성 여부를 판단하는 것이 필요하며 가급적 염색을 피하는 것이 좋을 것이다. 염색약에 발암물질이 포함되어있다는 보고도 있어 염색 제조사의 철저한 실험과 염색 물질의 선별이 염색으로 인한 부작용을 최소화하는데 중요한 역할을 할 것이다. Hair coloring has became one of the most popular cosmetic activities to younger generations during last decade. However, there are few studies on the health effect of widespread use of chemical dyes. This study was conducted to study the effects of hair coloring dye on hair and other systems. We conducted a questionnaire survey of 80 persons in Kyongju campus, Dongguk University. We have done open patch skin test on 46 medical students. We also conducted scanning electron microscopy to examine the hair strength and structure before and after hair coloring process. Injury of hair and hair bulb, contact dermatitis, turbid eyes, xerophthalmia, and poor visual acuity were the main symptoms complained after hair coloring (p<0.05). Injury of hair and hair bulb were increased by frequency of hair-dryer use(p<0.05). In open patch test, pruritus was complanined by more than half of the subjects. Mean strength of hairs before and after hair coloring was as follows; 134.5 (SD 10.37)g before hair coloring, 128.0 (SD 30.69)g immediately after hair coloring, and 112.5 (SD 19.69)g after two days. The scanning electron microscopic findings of hair surface before and after hair coloring showed decreased keratin layer and thinning of the hair. Hair coloring induces injury to hair, its keratin layer, and hair bulb as well as contact dermatitis, turbid eyes, xerophthalmia, and poor visual acuity. Therefore, we think that precaution is needed in use of hair coloring dye. To prevent complications induced by hair coloring dye, it is necessary, especially to those with allergy or skin disorders, to perform skin test before action and avoid hair coloring whenever possible. Longterm health effects of hairdye should be studied and manufacturing companies should try to minimize complications induced by hair coloring dye.