Protein microarray를 이용한 Apin-단백질의 상호작용에 관한 연구

박주철,박선화,김흥중,박종태,윤성호,김지웅,이태연,손호현 대한치과보존학회 2007 Restorative Dentistry & Endodontics Vol.32 No.5

비소세포폐암에서 Microsatellite Instability

전효성,김정란,손지웅,박선하,박태인,김창호,김인산,정태훈,박재용 경북대학교 병원 2001 경북대학교병원의학연구소논문집 Vol.5 No.1

연구베경:MMR 유전자의 불활성화에 의해 야기되는 뮤전적 불안정성은 발암기전의 한 부류로 인정되고 있다.저자들은 비소세포폐암의 발암과정에서의 MSI의 역할을 규명하기 위해 비소세포폐암에서 MSI의 빈도 및 MSI 유무에 따른 임상상의 차이를 조사하였다. 대상 및 방법:근치적 절제술을 받은 비소세포폐암 20예를 대상으로 하였다.동결된 폐암조직과 환자의 림프구에서 DNA를 추출한 후 3P와 9p의 15개의 marker들을 대상으로 PCR을 시행하고 7% polyacrylamide gel에서 전기영동한 후 silver 염색을 시행하였다. 암조직과 림프구 DNA의 PCR product의 band를 비교하여 MSI와 LOH를 판정하였다. 결과:1)대상환자들은 남자 19예,여자 1예였으며 모두 흡연자였고 평균 흠연력은 47 갑년이었다. 폐암의 조직형은 편평상피암 15예, 선암 4예,대세포암 1예였고, 술 후 병리학적 병기는 Ⅰ기 6예,Ⅱ기 5예, ⅢA기 7예,ⅢB기 2예였다. 2)20예 가운데 8예(40%)에서 MSI가 관찰되었으며 3예는 한 개의 marker에서,5예는 2개 이상의 marker에서 MSI가 관찰되었다. 3)LOH는 10예(50%)에서 있었으며,LOH유무에 따른 병가 및 흡연력의 차이가 없었다. 4)분석한 marker의 10% 이상에서 MSI가 관찰된 MSI-L종양은 5예였으며, 대부분의 marker에서 MSI양성인 MSI-H종양은 없었다.MSS종양과 MSI-L종양은 흡연력, 병기, 폐암 조직형 및 LOH 빈도의 유의한 차이가 없었다. 결론:비소세포폐암에서 MSI는 비교적 흔히 관찰되지만 MMR 유전자의 불활성화에 의한 MMP pathway 는 비소세포폐암의 주요 발생기전은 아닐 것으로 생각된다.향 후 비소세포폐암의 발암과정에 있어서 MMP pathway의 역할을 규명하기 위해서는 보다 많은 예를 대상으로 한 연구가 필요할 것으로 생각되며, MSI 발생기전에 관한 추가적인 연구가 필요할 것으로 생각된다. Purpose: Microsatellite instability(MSI) is frequently used as an indicator of microsatellite mutator phenotype (MMP) tumors.MSI has been observed in a percentage of non-small cell lung cancer(NSCLC).However, its role in tumorigenesis of NSCLC remains unknown.The frequency and partten of MSI in NSCLC were evaluted and clinical parameters of MSI-positive tumors with those of MSS(microsatellite stable) tumors were compared. Materials and Methods: Twenty surgically resected NSCLCs were analyzed for 15 microsatellite markers located at chromosomes 3p and 9p.The peripheral blood lymphocytes of patients were used as the source of the normal DNA. Results:1) of 20 cases, 8(40%) demonstrated MSI. 2) Instability was observed more frequently in tri- and tetra-nucleotide repeats that in dinucleotide repeats. In all cases, instability appeared as a shift of individual allelic bands. 3) LOH was observed in 10(50%) of 20 tumors analyzed. 4) Of 20 cases, MSI-H tumor(showing MSI in the majority of markers) was absent. There were 5 MSI-L tumors( showing MSI in a greater than 10% of markers). 5) No significant difference was observed between MSI-L tumors and MSI negative tumors in terms of clinicopathologic features such as pack-year history of smoking, histologic subtype, and (delete) stage of disease.There was also no significant difference in the incidence of LOH in relation to the status of MSI. Conclusion: These data strongly suggest that MSI plays different roles in lung and colon cancer.MMP pathway appears to be far less important in the tumorigenesis of NSCLC, caused mainly by cigarette smoke, with little familial tendency.(tuberculosis and Respiratory Diseases 2000,48:24-32)

블럭탐색 제어를 이용한 프랙탈 영상 부호화 시간 향상과 컬러 영상에의 응용

박철우,정동웅,김태형,김두영 東亞大學校 附設 情報通信硏究所 1996 情報通信硏究所論文誌 Vol.4 No.1

In this paper, in order to reduce block search area during the process to determine the coefficient of IFS transform equation in Fractal Coding, We performed the following steps : First, We partitioned Domain blocks and Range blocks with certain critical point determined by the edge characteristcs and the direction of brightness. When similar block's detected, the Domain block in the same class of the given range was only searched. Second, When the processing is on, We determined the linear list structure with the second error limit, We, therefore, could control coding time, We tryed to improve the compression rate of the Image with reducing the number of times of tranformation by changing the area of block with Quadtree Partitioning, and also tried to progress the fidelity of the image by changing the search density of search area, according to the magnitude of range block. We applied these method to image compression of 24-bpp color. First of all, after converting the RGB coordinate into YIQ Coordinate which has brightness signal and chrominance signal, We minimized by a quarter and coded I,Q signal which implies the information of the color but takes just small part at image information and on the case of decoding, with linear interpolation method we got I,Q signal enlarged as it was. All throughout these processing, We could find out that There are no damages on the original image, and we are able to make better result on compression rate and fidelity of image comparing with those of conventional Fractal Coding method which was applied to the RGB images.

발열로 인해 발현된 부루가다 증후군 1례

박혜연,김종훈,박경일,황철웅,김태년,남궁준,도준형,이원로,이성윤 白中央醫療院 2005 仁濟醫學 Vol.26 No.1

Brugada syndrome is characterized by right bundle branch block morphology and ST segment elevation in the right precordial leads and a propensity to develop ventricular arrhythmias. Mutations in a cardiac sodium channel gene have been linked to this syndrome, and the ionic mechanisms responsible for the electrocardiographic phenotype are temperature-dependent. This case report describes a patient in whom a typical Brugada ECG pattern developed during fever and returned normal ECG after fever was subsided.

필기체 숫자인식의 최적 신경망 구조 설계를 위한 개선된 유전자 알고리즘

박찬란,김향태,정재영,위승정,최봉한,최호진,이웅기 조선대학교 통계연구소 1999 統計硏究所論文誌 Vol.1 No.1

본 논문에서는 필기체 숫자인식의 최적 신경망 구조 설계를 위하여 유전자 알고리즘과 역전파 알고리즘을 병합하는 방법을 이용하였으며, 유전자 알고리즘은 유전인자의 역할에 따라 독립적으로 적응하는 개선된 선택, 교배, 돌연변이 연산자를 제안하였다. 실험결과는 유전인자의 역할에 적응하는 개선된 연산자들의 실행으로 최적의 신경망 구조를 설계하였고, 설계된 신경망 구조에서의 학습이 가장 좋은 성능을 나타내었다. In this study, the method of using both genetic algorithm and back propagation is proposed to design the structure of optimal neural network recognizing handwritten digits. Improved selection, crossover and mutation operator which can control the genetic algorithm independently according to the role of gene are used in the proposed method. Simulation result shows that the neural network of this method can search effectively the optimal hidden node numbers, connection weight, learning rate and the momentum because of above proposed operators.

퍼팅 동작 영상분석 시 프레스 동작의 이벤트 설정 가능성 여부

박태진,서국웅,서국은 釜山大學校 附設 體育科學硏究所 2009 體育科學硏究所 論文集 Vol.25 No.1

본 연구는 부산.경남지역에서 세미프로로 활동 중인 7명을 선정하여 퍼팅(10.94 m)동작 시 프레스 동작을 3차원 영상 기법을 통해 운동학적으로 비교.분석하여 얻은 결론은 다음과 같다. 첫 번째, 양손은 어드레스를 취한 후 백스윙되기 전까지 볼 진행 방향으로 움직이는 것으로 나타났다. 두 번째, 퍼터헤드, 몸통, 어깨 중심점은 프레스 동작 시 좌.우 방향으로 움직이지 않고 고정되어 있는 것으로 나타났으며, 프레스 시 양손 중심점은 어드레스 시 보다 임팩트 시에 가까운 것으로 나타났다. 세 번째, 퍼팅 스트로크 시 총소요시간 중 프레스 동작이 22.83%를 차지하고, 구간 중 다운스윙과 팔로스루 시간 보다 많이 소요되는 것으로 나타났다.

복잡한 배경에서 얼굴 영역 추출

박용성,유태웅 群山大學校 1996 論文集 Vol.23 No.-

Finding human faces automatically in a scene is a difficult yet significant problem. This paper proposes a method for detecting face regions from a complex background, and describes the experimental results. We perform face location based on the observation that human faces are characterized by their color information and texture features as follows. Firstly we construct a face chromatic histogram in hue and saturation chromatic space. Secondly we use the chromatic histogram to detect skin pixels from the input image. Thirdly a face texture model is constructed by calculating inertia, inverse difference and correlation of facial images. Finally the locations of the face region are determined by face texture features.

水稻品種에 따른 벼멸구 生態型의 攝食行動에 관한 硏究

裵泰雄,朴永道 東亞大學校生命資源科學大學附設 農業資源硏究所 1992 農業生命資援硏究 Vol.1 No.1

水稻品種에 대한 加害能力을 달리하는 벼멸구(Nilaparvata lugens Stal) 生態型에 대한 水稻品種의 幼苗 및 幼齡別 抵抗性反應을 調査하고, 또 벼멸구 生態型들이 우리나라에 얼마나 飛來해 오는지를 調査한바 그 結果는 다음과 같다. 1. 水稻幼苗의 抵抗性反應은 東津벼가 生態形-1,2,3 모두에 感受性이었고, 三剛벼와 密陽 63號는 生態型-2와 3에 各各 感受性이었다. 그러나 伽倻벼는 生態型-1,2,3 모두에 抵抗性이었다. 2. 苗齡에 따른 水稻品種別 甘露排泄量의 差異는 生態型에 따라 苗齡別로 多樣하게 나타났다. 3. filter paper위에 나타난 甘露排泄面績과 排泄된 甘露의 무게와는 서로 相關이 있었다. 즉 東津벼는 生態形-1,2,3에서, 三剛벼는 生態型-2에서, 密陽 63號는 生態型-3에서 甘露排泄面績과 무게가 모두 높게 나타난 反面, 伽倻벼는 生態形-1,2,3 모두 낮아 抵抗性反應을 보였다. 4. 벼멸구 生態型 分布比率은 採集時期 및 地域間에 약간의 差異는 있었으나, 生態形-1,2,3이 各各 57.0%, 25.1%, 18.0%였다. The reaction of the varietal differences in resistance to the brown planthopper(BPH), Nilaparvata lugens Stal, biotypes by using the rice seeding responses, the feeding amount of the three BPH biotypes fed on 30 to 70-day-old rice cultivars with different resistance genes, the distribution ratio of the BPH biotypes migrated from southern Chinese continent into the southern costal regions of Korea were tested. According to the reponses of rice seedling to the three BPH viotypes, Dongjinbyeo(no resistant gene)was shown highly susceptible to their nymphs, Samgangbyeo(Bph-1 gene)was susceptible to the biotype-2, and Milyang 63 was susceptible to the biotype-3, But Gayabyeo(Bph-and bph-2 gene)was hightly resistant to their nymphs, Based on the reaction of the varietal differences in resistance to the BPH biotypes on 30 to 70-day-old by using the honeydew excretion test, the rice cultivars with Bph-1 gene including Samgangby대 were resistant to biotype-1 and 3, but susceptible to biotype-2, and those with bph-2 gene like Milyang 63 were susceptible to biotype-3, but resistant to biotype-1 and 2, But those with Bph-1 and bph-2 gene like Gayabyeo were highly resistant to biotype-1, 2, and 3. The percent biotype distribution of the female BPH colleted in the southern coastal regions of Korea showed that the BPH biotype-1 was predominent accounting for 57.0%, biotype-2 was 25.1%, and biotype-3 was 18.0%

韓國産 裸予植物에 대한 系統分類學的인 硏究(槪要)

蘇雄永,宣炳崙,李相泰,朴相珍 全北大學校 生物學硏究所 1983 生物學硏究年報 Vol.4 No.-

This is the introductory paper of Systematic Studies of the Korean Gynmosperms. To elucidate the relationships among them, three parts works were performed ; external morphology, wood anatomy, and pollen morphology. As the results, three parts works were well consisted with the seperation of the Genus Pinus into two Subenus, Haploxylon and Diploxylon. And wood anatomical features strongly indicated that the Genus Cephalotaxus which was treated as a seperate family by some authors (Engler, 1926 ; Nakai, 1952 ; Cronquist et al., 1966) was different from the Genus Taxus and Torreya.

癩菌檢出에 對한 skin smear와 Nuclepore法의 比較

張明雄,崔大卿,朴成均 大韓癩學會 1980 癩學 會誌 Vol.13 No.1