RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향

박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.

반려동물의 기생충검사 이론 및 실제

이성호,박영석,박병권,김상기,정지윤,박수진,박창식,김병수 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

The aim of this article is to improve human health and that of companion animals by improving the diagnosis and control of parasitic diseases. This is achieved by research into parasites of veterinary significance including zoonoses and the use of comparative systems to increase our understanding of some human parasitic diseases. We should promotes learning and teaching through knowledge and operates a diagnostic unit that offers a service to pet owner for indigenous and exotic parasitic diseases of companion animals. We must diagnose and treat them early. Zoonotic parasites are very important because many of animal parasites are zoonoses and give pain and death to human. Parasites are divided Protozoa, Helminthes and Arthropods. There are many protozoa, about 65,000 species. Arthropods are the vector of another diseases, such as virus, bacteria, protozoa and nematodes. Helminthes are composed of Trematoda, Cestoda, Nematoda and Acanthocephala. The target of Veterinary Parasitology is to recognize the characteristics of these parasites and subsequently diagnose, treat and control them. This article is intended to familiarize you with the essential facts and concepts of companion animal parasitology. Emphasis is placed on fecal sample techniques (comparison of fecal examination techniques; frequently found artifacts in fecal smears; direct Smear; zinc sulfate flotation; etc.) We recommend that the study be done to more references on animal parasites.

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

ATIS 최적 프로브차량대수 모형개발

박성식,고승영,서준석 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-

To collect real-time link traffic information(Link Travel Time) for based on beacon and GPS technologies prove cars always have to be operate on the entire network. In this study, in case of taxi probe cars based with Beacon and GPS, method on determining required number of probe cars is studied to get traffic information of reality. Using taxis for probe cars, the the factors that influence the number of probe cars are operation time and distribution of prove cars over the network, operate distance and travel speed, the portion of revenue operation distance of taxis. The result of this study was ; As the collecting period for one traffic information prove cars travel more link, thus the number of prove cars decreases. The number of prove cars decreases when the allowable numer of links that no link travel times are collected increases.

産卵鷄에 대한 練炭灰添加給與 效果에 關한 硏究

朴成振,文勝式,鄭場龍 진주산업대학교 1982 論文集 Vol.20 No.-

This experiment was conducted to investigate the effect of Briquette ash treatment on egg production rate, egg weight, feed conversion and egg quality with date obtained from 100 pieces egg type fowls Babcock (B 380, 230days-age) fed and allotted 5 treatments at the rate of 0%(C), 0.5%(T_1) 1.0%(T_2), 1.5%(T_3)AND 2.0%(T_4) during the experiment period of 119 days from May 21, 1982 to September 20, the same year ar Department of Animal science, Jin Ju Agricultural and Forestry Junior Technical College. The results obtained are summarized as follows; 1. Average egg weight at each experiment plot was 62.21g(T_2) 61.62g(T_3), 60.88g(T_4), 60.42(T_1) and 60.04g(C), but the difference was not significant. Feed conversion at each experiment plot was 2.52(T_2) 2.60(T_3), 2.65(C) and 2.67(T_4). 2. Average egg production rate at each experiment plot was 82.02%(T_2) 81.85%(C) 81.43%(C), 79.41%(T_3) and 78.23(T_4), but the difference was not significant. 3. Weight of egg yolk and albumen were higher at fed with the treated Briquette ash plot than that without Briquette ash plot, and weight of egg shell and thickness were not significant.

Low doses of ionizing radiation suppress doxorubicin-induced senescence-like phenotypes by activation of ERK1/2 and suppression of p38 kinase in MCF7 human breast cancer cells

신재식,우상혁,이형찬,홍승우,유두현,홍석일,이왕재,이명석,진영우,안성관,진동훈,박인철 Sookmyung Women's University Research Institute of 2010 여성과 건강 Vol.6 No.1

응급진료시 여성환자의 소변검체 채취방법

박원녕,정상원,이종호,구홍두,심호식,김승호 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Background: Urinalysis is a useful laboratory test in the diagnosis of various diseases. In sampling for the urinalysis, there is much concern about contamination that can lead to misdiagnosis in the mid-stream urine sampling method. We conducted this study to determine, in terms of concordance of results and contamination in culture, whether there was any superiority in nod-stream sampling methods with or without disinfection measures compared to the catheterization method. Materials and Methods: We used three kinds of urine sampling methods sequentially, mid-stream non-clean catch, mid-stream clean catch, and catheterization, for ambulatory, non-pregnant, non-men-struating female patients who visited NHIC Ilsan Hospital emergency department during a one-week period in September 2001. Each sample was electrophotometrically analyzed for leukocyte esterase, nitrite, and blood by using a reagent strip and was cultured immediately or after overnight refrigeration. Results: Of the 41 patients, 9 had culture-proven urinary tract infections. The concordance rates(kappa) for nitrite, blood, and leukocyte esterase were 0.875, 0.403, and 0.406 between non-clean catch and catheterized samples and 0.875, 0.481, and 0.560 between clean catch and catheterized samples, respectively. The contamination rate of the non-clean catch, the clean catch, and the catheterized samples were statistically different: 51.2%, 29.3% and 0%, respectively. Conclusion: We found that cleaning with disinfectant was effective for reducing the contamination rate, even though the best urine sampling method for zero contamination was catheterization. We recommend considering cost, patients' comfort, an acceptable threshold for contamination, and the necessity for a culture before choosing a urine sampling method for women who visit the emergency department.

우리대학의 주문식교육 방향에 관한 연구

박정민,김태곤,이규식,신승우 慶北專門大學 2003 慶北專門大學 論文集 Vol.22 No.-

This study was conducted to establish an operational direction for customized training in our college. The demands for customized training were analyzed on paper and in person 37 junior colleges, 100 companies and 468 students. The interview results were formulated into educational policies on customized training in our college. From the these results, the reasonable direction to be taken by customized training would be to develop various training program that meet the demands of the industry and students. In order to do this, our college must analyze its own regional and social environment and develop a variety of programs.

유리纖維 補强 콘크리트 流動 特性에 관한 硏究

朴承範,任昌悳,尹義植 忠南大學校 産業技術硏究所 1989 산업기술연구논문집 Vol.4 No.1

Concrete is an excellent structural material, but it has many problems due to brittle behavior, weak resistance in tension, flexibility and toughness. In order to improve these problems, usually fiber reinforced concrete is used. But in the case of GFRC insufficient workability is caused by using glass fiber and there is lack of studying on it. This report of the following concrete of GFRC was experimented by the different W/C, S/A, glass fiber contents and maximum size of Aggregates. The results of this study is as follows; 1) Optimum S/A ratio is 0.6 at the glass fiber contents of from 0.25% to 0.75% in GFRC, but it cannot be obtained optimum S/A ratio at over 0.75. 2) Even though increasing the W/C ratio, we can get more slump, but slump is much more influenced by fiber contents in GFRC rather than increasing the W/C ratio. 3) Workability of GFRC is much more influenced by fiber contents than fiber length. 4) Workability of GFRC is a little improved by the bigger maximum size of aggregates.

순차적 크리깅기법을 이용한 미소혼합기의 최적화

박재용,유진식,황승민,임민규,오영규,김용대,한석영,맹주성 한국공작기계학회 2008 한국공작기계학회 추계학술대회논문집 Vol.2008 No.-

An active micro-mixer, which is composed of an oscillating micro-stirrer in the micro-channel to provide effective mixing was optimized. The effects of molecular diffusion and disturbance by the stirrer were considered with regard to two types of mixer models: the simple straight micro-channel and micro-channel with an oscillating stirrer. Two types of mixer models were studied by analyzing mixing behaviors such as their interaction after the stirrer. The mixing was calculated by Lattice Boltzmann methods using the D2Q9 model. In this study, the time-averaged mixing index formula was used to estimate the mixing performance of time-dependent flow. The mixing indices of the two models were compared. From the results, it was found that the mixer with an oscillating stirrer was much more enhanced' and stabilized. Therefore, an approximate optimization of an active micro-mixer with an oscillating stirrer was performed using Kriging method with OLHD (Optimal Latin Hypercube Design) in order to determine the optimal design variables. The design parameters were established as the frequency, the length and the angle of the stirrer. The optimal values were obtained as 1.5754, 0.803D and ±45°, respectively. It was found that the mixing index of the optimal design increased by 83.36% compared with that of the original design.