식물생장소가 대두 엽육세포의 소기관에 미치는 영향

朴弘悳 대구효성가톨릭 대학교 1983 연구논문집 Vol.27 No.1

Extreme points에 關하여 硏究함에 있어 제Ⅱ장에서는 本 論文에서 必要한 제 性質을 調査 硏究하고, 그 結果를 利用하여 제Ⅲ장에서는 Extreme points에 關한 整理를 밝혀 硏究하고자 한다. In order to investigate the changes of kinetin(KI), IAA, KI-IAA mixture, GA₃, 2, 4-D, and GA₃, 2, 4-D mixture, cell organelles in mature soybean mesophyll were observed. The endoplasmic reticulum were distended and fragmented by GA₃, 2, 4-D mixture and KI treatment. The grana and intergrana lamellae of chloroplast were swollen by IAA and 2, 4-D treatment. Mitochondria, ER, dibtyosome, ribosome, and multivesicular bodies in the cytoplasm were increased by KI-IAA mixture and GA₃treatment.

감자와 대두의 원형질체 분리, 융합 및 배양

朴弘悳 대구효성가톨릭대학교 1986 연구논문집 Vol.33 No.1

The purpose of this study was carried out to optimal conditions for isolation of protoplasts, fusion, and culture from Glycine max callus and Solanum tuberosum mesophyll. The enzyme solutions of protoplasts isolation from Glycine max callus was 2% onozuka, 0.5% pectinase, and 0.5% driselase and incubation time was 8hr., but Solanum tuberosum mesophyll was 0.5% onozuka, 0.1% macerozyme and incubation time was 6hr. The fusigenic agent of protoplast was treated with 50% polyethylen glycol(PEG) 1,500 for 50 min. The fused protoplasts were cultured in protoplast growth medium supplemented with 1μm NAA and zeatin at 26℃ under dim light. Regeneration of few cell walls occured after 24hrs in culture, but callus was not obtained. And the characteristics of cultures used in this study were discussed.

高等植物의 Protoplast 融合에 關한 硏究

朴弘悳 대구효성가톨릭대학교 1982 연구논문집 Vol.25 No.1

Cedrus derdara(Roxb.) Loud의 종자, Glycine max Merr cv, Bean Sprouts의 胚軸上端, Helianthus annuus의 種子 및 Platanus orientalis의 잎에서 각각 callus를 誘導시켰다. 이들 callus에서 分離한 原形質體와 Allium fistulosum의 葉肉組織에서 分離한 原形質體를 각각 融合시켰다. The callus tissues were induced from seed of Cedrus derdara(Roxb.) Loud, shoot pole of hypocotyl of Glycine max Merr cv. Bean Sprouts, seed of Helinhus annus L., and leaf of Platanus arientalis. The protoplasts isolated from these callus tissues were fused with protoplast isolated from Allium fistulosum mesophyll. And the characteristics of tissue cultures used in this study were discussed.

시금치 엽육세포에서 분리한 Chlorplast Protein에 미치는 IAA 효과

朴弘悳 대구효성 가톨릭 대학교 1984 연구논문집 Vol.29 No.2

시금치 엽육에서 분리한 엽록체 단백질과 IAA 와의 관계를 광처리 하에서 조사하고, 엽록체 단백질에서 각 단백질의 band 를 SDS-polyacrylamide gel electroporesis 로 추정하였다. The relation between IAA and chloroplast protein isolated from spinach mesophyll was examined under the light. In addition, each protein bands in chloroplast protein were separated by SDS-polyacrylamide gel electrophoresis.

배추 숙성 중 뿌리잎의 형태학적인 변화

박홍덕 효성여자대학교 부설 한국환경위생연구소 1994 환경위생연구 Vol.4 No.1

배추 숙성중 뿌리잎의 형태학적인 변화에 대한 결과는 다음과 같다. 1. 백색부위의 표피세포는 24시간처리부터 붕괴되고, 유세포는 팽창하였다. 2. 녹색부위의 표피세포는 144시간, 유세포는 48시간처리부터 붕괴되었다. This study was conducted in morphological changes during matura-tion of baechu(Brassica compestris L. ssp.napsus var.pekinensis Makino) root-leaf. The result were as follows : 1. Epidermis and parenchyma cell of white region begin to breakdown and expansion from treatment for 24hr. 2. Epidermis and parenchyma cell of green region begin to breakdown from treatment 144hr and 48hr.

배추의 형태학적 특성과 세포벽 다당류

박홍덕,김순동 대구효성가톨릭대학교 식품과학연구소 1997 식품과학지 Vol.9 No.-

김치논문에서의 배추에 대한 학명표기와 부위별 명명법이 매우 다양하며 통일된 표기법이 요망되었으며, 배추잎을 중록과 엽신으로 표기하기 보다는 백색부위와 녹색부위로 표기하는 것이 바람직한 것으로 판단되었다. 배추에서 백색부위의 도관에서는 환상, 계문상 및 망상비후로 구분되었다. 배추의 일반성분은 품종별로 상당한 차이가 있었으며, 세포벽 다당류의 함량도 품종간에 상당한 차이를 보였다. 배추의 세포벽 펙틴을 겔여과한 결과 4개의 피크로 분리되었으며 2개는 개략적인 분자량이 200백만 이상, 1개는 십만내외 1개는 1만이하를 나타내었다. 헤미셀룰로즈는 2백만과 1만으로 추정되는 2개의 픽크가 분리되었다. This study was conducted to examine the morphological characteristics and cell wall polysaccharides of Chinese cabbage(Brassica). First of all, the variety of scientific name and naming of parts of Chinese cabbage in the literatures of kimchi showed, which will unify marks. So, we propose not so much mid-rib and leaf blade of Chinese cabbage leaf as white part and green part, respectively. On the other hand, the forms of vessel elements of white part in Chinese cabbage consist in ring, sclariform and reticulate thickening. The proximate compositions and contents of cell wall polysaccharides of Chinese cabbage has significant differences between its cultivars. The cell wall pectin from Chinese cabbage exhibited four peaks with molecular weights of 2,000,000, about 100,000 and less than 10,000 by gel filteration chromatography. And hemicellulose did two peaks with molecular weights of 2,000,000 and 10,000.

지베렐린과 Putrescine이 대두 절간의 세포 신장과 분열에 미치는 영향

윤상숙,박홍덕 효성여자대학교 부설 한국환경위생연구소 1991 환경위생연구 Vol.1 No.1

대두 제1절간에 지벨렌린, putrescine, MGBG 및 지베벨린과 'Put 혼합 물질을 농도 및시간에 따라 처리하여, 각 피층의 유조직 세포 크기를 측정하고 세포신장과 분열부위를 관찰하였다. 세포 신장은10^-6M지베렐린의 경우 Ⅱ부위, 10^-2MPut은 I부위,10^-2M MGBG는 I부위이며,처리시간은 모두 60시간에서 세포신장이 가장 활발하였다. 지베렐린과 Put을 섞어 처리한 경우는 단독으로 처리하였을 때 보다 세포 신장에서 큰 효과가나타났으나, 세포분열에는 나타나지 않았다. 대두 첫번째 절간에서 세포 신장 부위와 분열부위는 일치하지 않았다. The zone of cell elongation and cell division was observed in the parenchyma cell of cortex in Glycine max when it was treated with Gibberellin(GA),Putrescine (Put), methylglyoxal-bis(guanylhydrazone) (MGBG) and GA+ Put. The cell elongation was observed in all parts and specially enhanced in Ⅱ,I and I zone of internode treated with GA, Put and MGBG, respectively. Theoptimal concentration was 10^-6M GA, 10mM Put and 10mM MGBG. The cellelongation was enhanced in 60hrs. The cell division was prospered in Ⅲ zone of internode treated with GA,Put and MGBG and the optimal concentration was 10-4M,10mM and 10mM indi-vidually. The cell division was enhanced in 60 hrs. The cell elongation was enhan-ced more GA+ Put than GA and Put independently, but it was not effectivelyin the cell division. The cell elongation zone was not consistent with cell division zone in thefirst internode of soybean.

요추간판 탈출증의 보존적 치료효과 분석

강점덕,박홍덕 대한정형물리치료학회 2001 대한정형도수물리치료학회지 Vol.7 No.2

This study was undertaken to find out the effect analysis of conservative treatment in of lumbar intervertebral disc. The data was performed to evaluate the effect of conservative treatment in 50 patients aging from 20 to 67 with lumbar intervertebral disc patients. Changes in low back pain score were evaluated by age, occupation, duration of symptoms, marital status, education period, self assessment. Duration of symptoms in over 7 months was 62.0%. 8.6 scores of complete recovery was changes in low back pain score of self assessment patients. In the multiple regression of risk factors to changes in low back pain score were correlated with age, education period, marital status, duration of symptoms(P<0.05). All patients were assessed by the low back pain protocol which was composed of symptom, clinical sign and activities of daily living scales before and after the treatment. Poor result could be predicted after the nonoperative treatment in the patient groups of changes low back pain score decreased with aging, duration of symptom over 7 months.

Finestructural Changes on Differentiation of Seed in Panax ginseng

Park, Hong-Duok 대구효성가톨릭대학교 1980 연구논문집 Vol.22 No.2

裂開되지 않은 種子에서부터 裂開된 種子를 5個月間 種子分化에 따라 構造的 變化를 觀察 하였다. 裂開되지 않은 胚에서 spherosome과 reserve fat 부위에서는 가끔 단백질의 결정체가 觀察되었다. 胚盛熱 4주後 shoot apex에서 色素體內 녹말과립은 증가하여 결국 amylplast로 分化되었다. 胚盛熱에 따라 胚乳의 aleuron grain에서는 단백질의 축적부위가 상이한 전자밀도가 높은 부위와 낮은 부위가 관찰되었다. The structural changes from the undehiscented seed to the dehiscented seed during maturation for five months were observed. In the undehiscented seed, an amorphous crystalloid structure was frequently present near the spherosome and reserve fat. In the dehiscentee seed, after four weeks during maturation, the starch grains within the plastids were increased in the shoot apex. After all these were developed into amyloplasts. According to the maturation, aleuron grain was present with alternating electron-parent and -dense region of the endosperm. And the characteristics of cell organelles in the seed were discussed.

An Exploratory Study Comparing Blood Metal Concentrations between Stroke and Nonstroke Patients in Koreans

Park Yeong-Chul,Park Hae-Mo,Ko Seong-Gyu,Lee Sun-Dong,Park Hong-Duok Korean Society of Environmental Health 2006 한국환경보건학회지 Vol.32 No.3

Various heavy metals have been known for causing ischemic stroke. In order to describe the causative relationship between the blood levels of various heavy metals and stroke patients, 116 patients with stroke and 111 patients without stroke were selected from one Oriental medical hospital in Wonju, Korea. Total of 9 kinds of metals such as As, Cd, Co, Cu, Hg, Mn, Ni, Pb, and Zn were analyzed in blood from patients with and without stroke. There were no significant differences in the means of metal concentrations between the stroke and nonstroke patients except for the mean of Co concentration. In the case of Co, the means for stroke and non-stroke patients were 0.44 ug/l and 0.40 ug/l showing a significant difference at the level of p-value=0.05. The odds ratios for each metal ranged from 0.96 to 2.86. Most odds ratios were not significant but the odds ratio for Co, $2.86{\pm}1.49$ was significant, indicating that Co increases the risk of stroke by 2.86 times. In order to identify the specific risk level of stroke increased by a multiple interaction of metals, regression coefficients and odds ratio for a pair or multiple pair of metals were reanalyzed. However, all of regression coefficients and odds ratios were not significant. In conclusion, Co showed the significant level in blood from patients with stroke. In addition, the odds ratio of stroke was significantly different from other metals. Thus, it is considered that Co among various metals analyzed in this study is the important metal for increasing the risk of stroke.