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Stabilization of Bacillus subtilis Lipase A by increasing the residual packing
Abraham1, Tajo,Pil Pack1, Seung,Je Yoo, Young Taylor Francis 2005 Biocatalysis and biotransformation Vol.23 No.3
<P>Introduction of well-packed residues to the interior of a protein structure could be considered as a stabilization strategy since the reduction of buried cavities might stabilize protein structure. In this study, the less-packed residues with no water-contact were selected as target sites for increasing residual packing. When Lipase A from Bacillus subtilis (179 amino acids) was used as a model system, 43 less-packed residues were initially considered by analyzing their residual packing value and residual exposure ratio. Among the 43 residues, small amino acids such as GLY and ALA were chosen as target sites. Packing increases of ALA to VAL and GLY to ALA were estimated, by molecular modeling, to give 0.5368∼⃒0.7433  kcal mol −1 stabilization. Mutants of Lipase A such as A38V, A75V, G80A, A105V A146V, and G172A were obtained via protein engineering. Thermostability assays revealed that A38V, G80A and G172V were the most stable mutants. This procedure for selecting the target residues for improved thermostability of Lipase A could be applied for improving the thermostability of other proteins and enzymes.</P>
최경덕(Kyeongduck Choi),유상길(Sanggil Yoo),강희선(Heesun Kang),박상민(Sangmin Pack) 한국자동차공학회 2007 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-
This paper discusses on development of a BMS(Battery Management System) for D-HEV(Diesel-Hybrid Electronic Vehicle) including algorithm of battery(Lithium) SOC(State of Charge) estimation. The algorithm accurately estimates the remaining battery capacity for automated battery management. In this paper, a BMS including technique for battery monitoring(Pack-Voltage, Pack-Current, Cell-Voltage, Pack-Temperature), communication method, digital input/output control, a battery thermal control, and battery protection method is developed.
백민식(Min-Sik Pack),이시연(Si-Yeon Lee),양보석(Bo-Suk Yang),최성철(Sung-Choul Choi),이재근(Jae-Geun Lee) 대한기계학회 2004 대한기계학회 춘추학술대회 Vol.2004 No.11
This paper presents a control plan of clearances for power plant steam turbine with the characteristics of internal clearances. Internal clearances on the interstage of blades and shaft gland seals on the steam turbine which are installed in tandem compound was changed when the rotor turned in the cylindrical sleeve bearing due to the generation of oil film wedge. This presented concern is very useful to prevent the rubbing damage of seal edge between the fixed and moving parts in steam turbine due to the misalignment at the rotating and stationary parts. This method is applied for the unbalanced clearances distribution to the left and right sides in the turbine casing. A considerable amount of unbalanced clearances distribution trend is determined according to the rotating speed of shaft, bearing size, type of journal bearing, bearing load, lubrication oil viscosity, oil temperature, oil pressure and surface roughness of shaft.
Pack, U Hyon,Kwon, Oh Yun,Park, Kyeong Won 한국공업화학회 1999 Journal of Industrial and Engineering Chemistry Vol.5 No.2
The silica-pillared H^+-magadiites were prepared by interlamellar base-catalyzed hydrolysis of tetraethylorthosilicate [TEOS, Si(OC₂H_5)₄] intercalated into the interlayers of H^+-magadiite. The interlamellar hydrolysis of TEOS were conducted between 10 min to 40 min in 0.00%, 0.05%, 0.10% and 0.20% NH₃-water solution, affording siloxane-pillared H^+-magadiites with gallery height of 24.8∼30.3Å. Calcination of samples at 538℃ in air resulted in silica-pillared H^+-magadiites with a large specific surface areas between 551 and 1020 ㎡/g, depending on the aging time and NH₃ concentration. The reaction in pure water and 0.05% NH₃ solution afforded optimum specific surface areas and mesopores with narrow pore size distribution. The reaction in 0.10% and 0.20% NH₃ solution resulted in sharp decrease of specific surface areas and poorly-ordered basal spacings, implying the partial destruction in the layered structure of H^+-magadiite.