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- 저자 : Othman Ayu Nazreena (검색결과 1 건)
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Rahman Zuraida Abd,Seman Zulkifli Ahmad,Othman Ayu Nazreena,Ghaffar Mohamad Bahagia Ab,Razak Shahril Ab,Yusof Muhammad Fairuz Mohd,Nasir Khairun Hisam,Ahmad Khairulmazmi,Chow Yeow Lit,How Teo Chee,Saa 한국식물생명공학회 2022 Plant biotechnology reports Vol.16 No.3
The current study recognised the issues encountered in regenerating Malaysia MR219 rice plantlet via microspore culture and attempted to develop an efficient protocol in overcoming the restraints. In the present study, a high proportion of uninu- cleate microspores (49.17%) was isolated from Stage 2-Segment II panicle (59–61 days), which also exhibited the highest callus initiation rate of 8.50%. Maintenance of the panicles under a cool temperature of 4 °C for 7 days before isolating the microspores, resulted in the highest microspore viability of 58.33% and callus initiation rate of 9.33%. The microspore isola- tion protocol was also optimised in the present study. The filtration sieve engagement with a pore size of 80 µm and further suspension centrifugation at 800 rpm for 5 min produced the highest microspore viability percentage and callus initiation rate. The incorporation of 3.0 mg/L kinetin in conjunction with 0.5 mg/L 2,4-D greatly enhanced the callus initiation rate, with 11.33%. The callus proliferation capacity, with the formation of 481.67 mg callus, was significantly promoted by the addition of 1.0 mg/L kinetin and 0.5 mg/L 2,4-D into the growth medium. Moreover, a higher green plantlet regeneration frequency of 2.83% was induced by the supplementation of 8% sucrose, which produced an average of 3.50 green plantlets.
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