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The Treatment of Linear and Narrow Scar after Craniotomy Using the Follicular Unit Excision
Hiromi Okochi,Masamitsu Onda,Akira Momosawa,Masayuki Okochi 대한성형외과학회 2022 Archives of Plastic Surgery Vol.49 No.6
Background The scar alopecia after cranioplasty (SAC) may decrease the patient’s quality of life.We have treated SAC using follicular unit extraction (FUE). The aimof this study was to discuss that efficacy of FUE and how much hair follicular unit (FU) should be transplanted intraoperatively for the treatment of SAC. Methods We treated 10 patients (4 men and 6 women) who had SAC using FUE. Results The average age, alopecia size, and intraoperative hair density on the graft area were 29.812.1 years, 29.844.5cm2, and 34.611.8 FU/cm2, respectively. One year postoperatively, the average hair survival rate on the graft area was 66.36.1%. Hair appearance was rated as good in six, fair in three, and poor in one. Among patients whose 1- year postoperative hair density was 20 FU/cm2, five of six patients achieved good results. However, among patientswhose 1-year postoperative hair densitywas<20 FU/cm2, all four patients achieved fair or poor results. The postoperative hair densitywas significantly higher in patients whose 1-year postoperative hair densitywas20 FU/cm2 than in patients whose 1-year postoperative hair density was<20 FU/cm2. The rate of achieving fair or poor results was significantly higher if the postoperative hair densitywas<20 FU/cm2 than if it was20 FU/cm2 (p¼0.047). Conclusions FU excision is useful for the treatment of scar alopecia after craniotomy. Our results suggest that the 1-year postoperative hair density should exceed 20 FU/cm2 to achieve good outcomes.
Shuhei Yamamoto,Mina Okochi,Kowichi Jimbow,Hiroyuki Honda 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.3
Three-dimensional (3D) cell culture arrays of melanoma cell spheroids were assembled to evaluate the combined effect of a melanogenesis-targeting drug, Npropionyl- 4-cysteaminylphenol (NPrCAP), and heat treatment. An array-like multicellular pattern of mouse melanoma B16F1 cells in a collagen gel was established by magnetic cell labeling using a pin-holder device to exert a magnetic force. The cellular spheroids were exposed to NPrCAP and heat (42°C for 1 h) as a model of anti-cancer treatment. As a result, melanogenesis of B16F1 cells was 29-fold higher in this 3D array than in conventional two-dimensional (2D) monolayer cultures. Because the spheroid size was linearly correlated with the cell number within a spheroid, the antiproliferative effect could be evaluated in a non-destructive manner. Moreover, the half-maximal inhibitory concentration of NPrCAP coupled with heat treatment calculated from the spheroid size was 2-fold higher in the 3D array (0.30mM) than in 2D culture (0.15 mM). These results indicate that spheroid formation decreases the chemosensitivity of cancer cells, and this model would be suitable as a susceptibility assay for melanogenesis-targeting drugs. Therefore, this 3D culture model provides a better screening format to evaluate drug and physical treatments for cancer therapy than 2D formats.
Development of a Tactical Screening Method to Investigate the Characteristics of Functional Peptides
Akiko Kume,Mina Okochi,Kazunori Shimizu,Yasuko Yoshida,Hiroyuki Honda 한국생물공학회 2016 Biotechnology and Bioprocess Engineering Vol.21 No.1
Using spot-synthesized peptide arrays, a functional peptide can be screened as a high-binding peptide for a target molecule. We have developed a rational screening method for functional peptides by analyzing the physicochemical rules of high-binding peptide sequences. To screen the peptides simply and strategically, we prepared an exhaustive 4-mer peptide library consisting of 256 peptides (44 = 256) characterized by four physicochemical groups of 20 amino acids: Group 1, non-charged hydrophobic amino acids; Group 2, non-charged hydrophilic amino acids; Group 3, positive-charged hydrophilic amino acids; Group 4, negative-charged hydrophilic amino acids. First, our previous screening data from cell adhesion, bile acid-binding, and nanoparticle-binding peptides were applied to the fourcategory analysis, and target-specific physicochemical characteristics were obtained. We then prepared an exhaustive 4-mer peptide library using these four physicochemical groups, and screened for high-binding peptides that bind model proteins interleukin-2 and IgG. We obtained individual physicochemical rules for high-binding peptides: group 1 or 4 amino acids in position (P) 1, group 1 in P2 and P4 for IL-2, and group 2 and 3 amino acids at all position for IgG. Therefore, this system, which employs the use of a simple and strategic peptide library, will be useful in the development of functional peptides.
Kojima, Yuki,Inazu, Koji,Hisamatsu, Yoshiharu,Okochi, Hiroshi,Baba, Toshihide,Nagoya, Toshio Korean Society for Atmospheric Environment 2010 Asian Journal of Atmospheric Environment (AJAE) Vol.4 No.1
The effectiveness of the government regulation on tail-pipe emission for diesel vehicles issued in 2003 in Tokyo was evaluated in this study. Variations in annual average concentrations of polycyclic aromatic hydrocarbons (PAHs) and nitro-PAHs associated with airborne particulate matter were investigated in connection with the variation in airborne elemental carbon (EC) concentration in downtown Tokyo, Japan in 2006-2007 and in 1997-1998. The annual average concentrations of EC, seven different PAHs, and 1-nitropyrene were found to have decreased significantly from 1997-1998 to 2006-2007. The most prominent decrease in atmospheric concentration was observed for 1-nitropyrene, which is a representative nitro-PAH originating from diesel vehicles. This indicated that the government control has worked to considerably reduce both atmospheric mutagens and airborne particulate matter. In contrast, the concentrations of two nitro-PAHs, 2-nitrofluoranthene and 2-nitropyrene, remained the same. These nitro-PAHs are known to be formed by atmospheric nitration of their parent PAHs, and this result suggested factors other than the concentration of parent PAHs and $NO_2$ affects the degree of atmospheric formation of nitro-PAHs.
Naoya Katsumi,Shuhei Miyake,Hiroshi Okochi 한국대기환경학회 2018 Asian Journal of Atmospheric Environment (AJAE) Vol.12 No.2
We measured 1H and 13C nuclear magnetic resonance (NMR) spectra of Humic-like substances (HULIS) in urban atmospheric aerosols isolated by diethylaminoethyl (DEAE) and hydrophilic-lipophilic balance (HLB) resin to characterize their chemical structure. HULIS isolated by DEAE resin were characterized by relatively high contents of aromatic protons and relatively low contents of aliphatic protons in comparison with HULIS isolated by HLB resin, while the contents of protons bound to oxygenated aliphatic carbon atoms were similar. These results were consistent with the results of the 13C NMR analysis and indicate that hydrophobic components were more selectively adsorbed onto HLB, while DEAE resins selectively retained aromatic carboxylic acids. Furthermore, we demonstrated that the chemical structural features of HULIS were significantly different between spring and summer samples and that these disparities were reflective of their different sources. The estimated concentrations of HULIS in spring were found to be regulated by vehicle emissions and pollen dispersion, while the behavior of HULIS in summer was similar to photochemical oxidant and nitrogen dioxide concentrations. The proportion of aliphatic protons for summer samples was higher than that for spring samples, while the proportion of aromatic protons for summer samples was lower than that for spring samples. These seasonal changes of the chemical structure may also involve in their functional expression in the atmosphere.
Kono Maori,Okuda Tomoaki,Ishihara Nami,Hagino Hiroyuki,Tani Yuto,Okochi Hiroshi,Tokoro Chiharu,Takaishi Masayuki,Ikeda Hidefumi,Ishihara Yasuhiro 한국독성학회 2023 Toxicological Research Vol.39 No.1
Fine particulate matter (PM2.5) exposure has a risk of inducing several health problems, especially in the respiratory tract. The skin is the largest organ of the human body and is therefore the primary target of PM2.5. In this study, we examined the effects of PM2.5 on the skin using a human 3-dimensional cultured epidermis model. PM2.5 was collected by cyclonic separation in Yokohama, Japan. Global analysis of 34 proteins released from the epidermis revealed that the chemokines, chemokine C-X-C motif ligand 1 (CXCL1) and interleukin 8 (IL-8), were significantly increased in response to PM2.5 exposure. These chemokines stimulated neutrophil chemotaxis in a C-X-C motif chemokine receptor 2-dependent manner. The oxidative stress and signal transducer and activator of transcription 3 pathways may be involved in the increased expression of CXCL1 and IL-8 in the human epidermis model. Interestingly, in the HaCaT human keratinocyte cell line, PM2.5 did not affect chemokine expression but did induce IL-6 expression, suggesting a different effect of PM2.5 between the epidermis model and HaCaT cells. Overall, PM2.5 could induce the epidermis to release chemokines, followed by neutrophil activation, which might cause an unregulated inflammatory reaction in the skin.