Hepatic Cirrhosis Occurring in a Young Woodchuck(Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus(WHV)

Kyu-shik Jeong,정원일,Jae-yong Chung,Mi-young An,Chae-yong Jung,Gyoung-jae Lee,Jong-soo Kang,Byeong-cheol Kang,Young-heun Jee,Bruce H Williams,Young-oh Kwon,Da-hee Jeong 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Cirrhosis Occurring in a Young Woodchuck (Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus (WHV)Da-hee Jeong, Won-il Jeong, Jae-yong Chung, Mi-young An, Chae-yong Jung, Gyoung-jae Lee1, Jong-soo Kang1, Byeong-cheol Kang2, Young-heun Jee3, Bruce H Williams4, Young-oh Kwon5 and Kyu-shik Jeong*College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea1Shinwon Scientific Co., LTD, Research Institute, Suwon, Korea2Clinical Research Institute, Seoul National University, Seoul 110-744, Korea3College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea

Oxalate와 Enrofloxacin투여한 랫트신장에 대한 병리조직학적관찰

오원석,이차수,오규실,정원일,정재용,정다히,정규식 한국임상수의학회 2003 한국임상수의학회지 Vol.20 No.4

To investigate the renal effects of enrofloxacin administration on rats induced with dehydration or hyperoxaluria, male rats were treated with enrofloxacin of 50 mg to 500 mg/kg b.w.. The microscopical observations of kidney and urine sediment were carried out in the experimental groups. The result obtained were as follows; The male rats deprived of water for 72 hours and administered with enrofloxacin. As enrofloxacin administration dose was increased, clinical signs such as loss of appetite, depression, weakness, and loss of urine output became more severe. In the histopathological findings, there were hyperemia and hemorrhage in renal cortex, vacuolation and necrosis of renal tubular epithelia, proteinous casts within renal tubules. The male rats were orally administered with sodium oxalate and injected with enrofloxacin for 7days. As enrofloxacin administration dose was increased, clinical signs such as the loss of appetite and water consumption, and weakness became more severe. In the histopathological findings, there are hemorrhage of glomeruli and cortical hyperemia, vacuolation and necrosis of tubular epithelia, proteinous casts in renal tubules. In the microscopical findings of urine sediment, there are calcium oxalate crystal (diamond-like type) and magnesium ammonium phosphate crystals (rhomboid). The male rats were intraperitoneally injected with sodium oxalate and administered with enrofloxacin for 7days. As enrofloxacin administration dose was increased, clinical signs such as the loss of appetite and water consumption, weakness were more severe. In the histopathological findings, there were hyperemia and hemorrhage in both glomeruli and renal cortex. Severe necrosis of renal tubular epithelia, bluish materials within renal tubules were also found. In the microscopical findings of urine sediment, there were many calcium oxalate crystals. The present results suggest that enrofloxacin has some injurious effects in rats having dehydration or hyperoxaluria, and clinically, we should consider these renal injury effects when we use enrofloxacin in patients accompanied renal disease, dehydration and hyperoxaluria conditions.

인스트루먼트 패널용 수용성 질감 도료의 물리적 성질

김억(Ok Kim),오우정(Woo-Jeong Oh),전용(Yong Chun),김영태(Young-Tae Kim),안재범(Jae-Beom Ahn),조영호(Youngho Cho) 한국자동차공학회 2005 한국자동차공학회 춘 추계 학술대회 논문집 Vol.2005 No.11_3

Physical properties of waterborne soft feel coatings for instrument panel were investigated. Soft feel coatings give warm and velvet touch for cold and hard plastic materials. Waterborne soft feel coating is composed with two parts. Part A contains polyester polyurethane dispersion (PE-PUD), OH-functional water dispersible polyurethane resin (PC-PUD) and OH-functional water dispersible alkyd resin (OH-PA). Part B contains hydrophilic hexamethylene diisocyanate trimer (HHDI). Soft feeling of coated panel was related with the amounts of PC-PUD and OH-PA. The physical properties of coated panel could be controlled by the NCO/OH ratio from Part A and Part B. Soft feeling was enhanced with relevant to PE-PUD content but the content of PE-PUD was limited because of weakening of coated film strength. For excellent touch and good property, the optimum composition of soft feel coat was PC-PUD(30%) and PE-PUD(20%). The results from all reliability tests satisfied soft feeling, scratch, chemical resistance and fogging.

선박수리업무에 종사하는 군무원들의 작업환경에 대한 인식이 직업만족도에 미치는 영향

오경재,모영하,윤한,김명선,이정미,권근상 한국산업위생학회 2003 한국산업보건학회지 Vol.13 No.1

This study was carried out to evaluate the relations among working environments, health behaviors and satisfaction with their duty in a group of 200 ship-repair workers in the ROK navy. The results were as following; Age of workers was 40 years and over(83%) and working duration was 10 years and over (71.5%). Most of them were married and had studied beyond high school. The 69.5% of workers satisfied with their job, whereas. about 59.5% of workers had satisfaction with their working condition. There were current drinkers, 78.5%, current exercising workers, 43.5%, obese workers, 28.0%, and salted food intakers, 18.5% in the workers. The major harmful environmental factors in the ship-repair workplace are noise, vibration, dust and chemicals. Most of workers are exposed to above harmful factors and complained about exposure of noise and dust. The consumption of cigarette or alcohol in people who exposed to noise was much higher than in people who did not, but the difference was not statistically significant. Prevalence of obesity and hypertension were not significantly different between the exposure with noise and dust. Noise or dust exposure was significant relation to satisfaction with their duty and working condition. The affective factors for satisfaction with their duty were people who worked for 10 years or more(OR= 4.53), exercisers(OR=4.78) and dust(OR=3.05) in Logistic regression analysis.

혈액투석 환자에게서 발생한 재생불량성 빈혈 1예

정지용,윤나라,강대웅,오종찬,장재현,선길홍,박경희,정종훈,김현리 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.1

Aplastic anemia is a disease characterized by pancytopenia in the peripheral blood and hypocellular bone marrow with the fatty infiltration. The causes of the acquired aplastic anemia were usually related to drugs and immune-related diseases. A 34-years old man was admitted due to general weakness. He has been going hemodialysis for 2 years because of end stage renal disease (ESRD) due to chronic glomerulonephritis. At admission, laboratory tests showed hemoglobin 6.2 g/dL, hematocrit 18.0 %, WBC 5,710mm (neutrophil 71.8%, lymphocyte 19.1%, monocyte 5.9%), reticulocyte 1.9%, platelet 93,000/mm, Fe 12.8 ug/dL, TIBC 204 ug/dL, ferritin 941.47 ng /ml, haptoglobin 0.72 g/L, vitamin B12 508.17 pmol/L, folate 24 ng/mL, total protein 6.54 g/dL, albumin 3.76 g/dL, Alk. pohsphatase 79 IU/L, AST 30.0 IU/L, ALT 39.7 IU/L, total bilirubin 0.57 mg/dl, BUN 79.4 mg/dl and creatinine 10.18 mg/dl. Peripheral blood smear showed the normocytic normochromic anemia with anisocytosis. Anemia was diagnosed but it was irresponsive to the recombinant human erythropoietin (EPO) and blood transfusion. In the bone marrow aspiration smear were there the hypoplastic myeloid and erythroid precursors. The bone marrow biopsy section showed the hypoplasia of all components (10-20%) and the fatty infiltration. We have experienced one case of the idiopathic aplastic anemia in a patient going through hemodialysis and we just report it with documentary records. 저자들은 혈액 투석 중인 환자에서 약물, 면역계 이상등의 원인을 발견할 수 없었음에도 재생 불량성 빈혈이 발생한 1예를 경험하여 문헌 고찰과 함께 보고하는 바이다.

배관 결함부 원거리장 와전류 신호 정량화 연구

정진오,이재경,김형진 한국비파괴검사학회 2000 한국비파괴검사학회지 Vol.20 No.6

다양한 기계 가공 결함을 유기한 공칭 외경 100mm의 덕타일 주철관을 대상으로 원거리장 와전류 검사를 수행하였다. 상수도 배수관으로 사용되는 주철관은 제조 단계의 큰 허용 오차 때문에 두께가 일정하지 않고 단면이 비대칭적인 특징을 지니므로, 원거리장 와전류 탐상 신호에는 배관 축방향에 걸친 장범위 잡음이 존재하게 된다. 본 논문에서는 장범위에 걸친 배경잡음을 효과적으로 제거하기 위하여 이동구간 평균법을 응용하였고, 결함의 깊이와 원주방향 정도를 정량적으로 평가하기 위하여 voltage plane 극좌표(VPPP) 방법을 활용하였다. 이동구간 평균처리로서 신호비를 일차적으로 향상시킨 다음 VPPP 기법을 이용하여 결함신호를 얻은 결과, 결함신호가 VPPP상의 기준점에서 x 축과 이루는 각이 결함 깊이와 직선적인 상관 관계가 있음을 확인하였다. 따라서 배관과 동축으로 놓인 탐촉코일을 이용한 원거리장 와전류 검사로서 매설 상수도 배관 내ㆍ외부에 존재하는 부식결함을 비파괴 정량평가할 수 있는 기반을 구축하였다. The remote field eddy current (RFEC) inspection was performed on the ductile cast iron pipes with nominal outer diameter of 100mm, which were machined with various shapes and sizes of defects. Ductile cast iron pipes which are used as water supply pipe have the non-uniform thickness and asymmetric cross section due to relatively high degree of allowable errors during the manufacturing processes. These characteristics of ductile cast iron pipes cause the long range background noises in RFEC signals along the pipe. In this study, the machined defects in pipes were effedctively classified by the moving window average (MWA) method which eliminated the long -range noise. The voltage plane polar plots (VPPP) signatures showed method was used to quantitatively evaluate the depth and circumferential degree of defects. The VPPP signatures showed that the angle between defect signature and the naormalized in-phase component on the VPPP is linear to the depth of defects. The nondestructive RFEC technique proved to be capable of quantitatively evaluating the machined defects of underground water supply pipe.

단계적 온도 하강법을 이용한 췌도세포 냉동보존법

정인경,오승훈,김병준,양태영,이병완,하창영,노정현,정재훈,민용기,이명식,이문규,김광원 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.26 No.1

연구배경:최근 당뇨병의 새로운 치료법으로 시도되고 있는 췌도이식은 충분한 췌도수의 확보와 췌도생존율을 높이기 위한 면역억제제 사용이 제한점이 되고 있다. 본 연구의 목적은 이식 전 충분한 췌도 수의 확보를 위해 분리한 췌도를 냉동보존하는 방법을 확립하고 냉동보존한 백서 췌도세포의 시험관내 그리고 생체내 기능을 조사하였다. 방법:분리한 백서의 췌장소도를 48시간 배양한 후 한 시험관당 췌도세포 1000개씩 나누었다. 냉동보존은 6개의 시험관에 DMSO를 첨가한 후 초 냉각(supercooling), 핵화(nucleation)단계를 거친 후 99% isopropanol과 액체질소가 들어있는 dewer를 이용하여-0.25℃/분의 냉각속도로 -40℃까지 단계적으로 얼린후-70℃ 액체질소 탱크에 보관했다. 해동은 냉동시킨 vial들을 액체 질소 태으에서 꺼내 37℃ 항온조에 담가 급격히 해동시킨 후, 원심분리하여 상층액을 제거하고 각 vial에 0.75M sucrose 용액을 가한 후, 10% fetal calf serum이 함유된 RPMI 1640 media에서 배양하였다 각각 6개의 시험관에서 해동한 췌도들을 광학현미경 및 형광현미경하에서 췌도의 모양과 생존율에 대해 조사하고 인슐린 정적반응을 알아보았다. 또한 분리한 췌도를 냉동보존하지 않고 이식한 경우를 대조군(6마리)과 생체내 기능을 비교하였다. 결과:① 냉동보존후 획득한 췌도의 수와 생존율 해동후 획득한 췌도의 수는 해동시킨 당일날이 902±21, 24시간 배양 후에는 857±16, 72시간 후에는 817±18개로 점차 감소되었다. AO/PI 염색상 각 췌도의 생존율은 냉동 전을 100으로 하였을 때 해동당일, 24시간 후, 72시간 후가 각각 60±5, 80±5, 90±5%로 해동후 3일간 배양하였을 때 냉동전의 수준으로 회복하였다. ② 냉동보존후 췌도의 포도당에 대한 정적 인슐린 분비능:냉동직후 감소된 경향을 보였으나 해동후 3일간 배양한 췌도의 인슐린 분비는 냉동전과 통계적으로 의미있는 차이가 없이 냉동보존 전의 수준으로 회복되었다. ③ 냉동보존후 췌도의 포도당에 대한 동적 인슐린 분비능:냉동보존한 췌도를 해동후 3일째의 인슐린 동적 분비능은 냉동 전과 마찬가지로 자극 인슐린의 반응의 제1기와 2기가 잘 관찰되었다. ④ 냉동보존한 췌도세포 이식 후 혈당 변화:스트렙토조토신으로 유도된 당뇨병 쥐에 췌도이식 후 혈당은 냉동보존한 췌도이식군에서 대조군에 비해 혈당의 조절효과가 더 오래 지속되었다. 결론:소동물에서 단계적 온도 하강법을 이용한 췌도세포 냉동보존법을 확립하였으며 이는 기능, 구조 및 생존율에 큰 이상을 보이지 않았으므로 장차 사람의 췌도세포 동종이식시 부족한 췌도세포수를 극복하고 면역반응을 줄일 수 있는 매우 유용한 방법이 될 것으로 판단된다. Background : Although islet transplantation has been attempted to reverse the state of diabetes, achieving a critical number of islets and modulating the immune response limit the success of islet transplantation. Cryo-preservation of islets offers many important benefits for islet transplantation by collecting islets with a wide variety of HLA phenotypes and islet MHC expression. The aims of this study was to determine the optimal conditions for cryo-preservation by using a controlled cooling method and to evaluate in vitro and in vivo functional properties of the cryo-preserved islets. Methods : Collagenase-isolated, Ficoll-purified islets were cultured for 48 hours. They were aliquoted into freezing tubes (1000 islets per tube), equilibrated with 2M dimethyl sulfoxide (DMSO) in three steps, supercooled, nucleated, and controll-cooled at rate of 0.25℃/min to - 40℃ prior to storage at - 196℃. Rapid thawing and removal of DMSO with 0.75 M sucrose preceded 48 hour of culture and the morphology, viability, glucose-induced insulin secretion, and in vivo function of rats transplanted with cryopreserved islets was reexamined. Results : ① Recovery was 90.2±0.2%, 85.7±0.1% and 81.7±0.1% immendiately after, 24 hours and 72 hours after thawing respectively. The viability was 60±5%, 80±5%, 90±5% immediately after, 24 hours and 72 hours after thawing respectively. ② The glucose-stimulated-insulin secretion (GSIS) tended to decrease immediately after thawing, but GSIS increase to the level of pre-cryopreservation 72 hours after thawing. ③ The in dynamic GSIS, the first and the second phase of insulin secretion were well preserved in islets cultured for 72 hours after thawing. ④ The cryopreserved islets were cultured for 3 days and transplanted into renal sub-capsular space of streptozotocin (STZ) induced diabetic rats. The duration of normoglycemia in the STZ-induced diabetic rats transplanted with cryopreserved islets was significantly longer than of the fresh islets. Conclusion : The optimal condition of cryopreservation using the controlled cooling method was established in rat pancreatic islets. This cryopreservation method can be a feasible approach for human islet transplantation (J Kor Diabetes Asso 26:64~74, 2002).

결핵균 약제감수성 검사의 비용효율성에 관한 다기관 연구

정석훈,이대동,최재철,김선주,신정환,정윤성,이은엽,오승환,배길한,장철훈 대한감염학회 2005 감염과 화학요법 Vol.37 No.1

목적 : 우리나라에서의 결핵균 감수성 검사는 검출 균주의 일부에서만 시행되고 있다. 본 연구에서는 모든 검출 균주들에 대해서 감수성검사를 실시하면서, 진료에 필요한 결과를 충분히 제공하고 경제적이면서 현실적인 감수성검사 방안을 제시하고자 한다. 재료 및 방법 : 5개 대학병원에서 일정기간 연속적으로 의뢰된 502균주의 감수성 결과를 분석하였다. 검사결과의 해석 및 2차 약제 감수성검사의 필요성은 NCCLS approved standard M24-A의 권고 기준에 따라 판단하였다. 결과 : 최소한 1가지 이상의 약제에 내성을 보인 경우는 초치료 환자의 10% (38/363), 재치료 환자의 61%(85/139)였으며, 다약제 내성을 보인 경우는 초치료 환자의 3% (11/363), 재치료 환자의 44% (61/139)였다. NCCLS 권고에 따라 2차 약제에 대한 감수성 검사를 시행하지 않아도 되는 경우는 초치료 환자의 96%, 재치료환자의 47%였다. 결론 : 초치료 환자는 95%에서 1차 약제에 대한 검사만으로 충분하므로 필요한 경우에만 2차 약제를 추가로 검사하고 재치료 환자에서는 1, 2차 약제를 동시에 검사할 필요가 있을 것으로 생각한다. Background : The anti-mycobacterial susceptibility test is performed on only a small percentage of clinical isolates in Korea. The aim of this study is to propose an anti-mycobacterial susceptibility testing scheme, which is not only economic and practical but also fully informative to physicians. Materials and Methods : The anti-mycobacterial susceptibility test results of 502 strains, isolated from five university-affiliated hospitals, were analysed. The interpretation of the results and the need for second-line drug susceptibility test were judged according to the recommendation of NCCLS M24-A guidelines. Results : The isolates from 10% (38/363) of treatment-navie patients and 61% (85/139) of retreatment patients showed resistance to at least one of the anti-mycobactial agents; 3% (11/363) and 44% (61/139) of isolates from each group were multi-drug resistant. According to the recommendation by NCCLS, the percentage of patients not needing the susceptibility test results for second-line drugs were 96% for treatment-naive and 47% for re-treatment patients. Conclusion : Since the susceptibility test against first-line drug is sufficient for 95% of treatment-navie patients with tuberculosis patients, susceptibility test against second-line drugs may be performed only when it is necessary. As for the re-treatment patients with tuberculosis, susceptibility test for both first-line and second-line drugs should be performed simultaneously.

동일한 혈관 내에 발생한 이식형 약제 주입기 카테터의 위치이상

오대석,김영재,김명훈,조광래,임세훈,이정한,이근무,정순호,신치만 仁濟大學校 白病院 2010 仁濟醫學 Vol.31 No.-

저자들은 56세 여성 환자의 왼쪽 쇄골 하 정맥에 시행한 이식형 약제주입기 카테터의 삽입 후 발생한 비정상 위치에 대한 증례를 보고하고자 한다. 카테터의 끝은 환자의 늘 어진 가슴, 체위, 신체의 움직임, 카테터의 유동성 등의 영향으로 천자된 정맥 내에서 견인되고 뒤쪽으로 이동하였다. 환자는 어떠한 증상도 호소하지 않았지만 비정상 위치의 카테터는 심각한 합병증을 유발할 수 있기 때문에 제거되었다. 따라서 시술자는 약제주입기 삽입 전에 환자의 신체 상태와 단순 흉부 촬영상을 확인하고 카테터의 비정상 위치가 발생할 수 있는 위험 징후를 알아차려야 하겠다.

광양만 광역도시계획 권역 교통수요예측에 관한 연구

오재화,정영덕 朝鮮大學校 建設技術硏究所 2006 建設技術硏究 Vol.26 No.1

Although there have been systemic efforts of the government to solve the metropolitan transportation problems, demand and supply for transportation facilities are not matched, yet. Scenario analysis by integrating land use and transportation has been popular in developed countries as a tool of metropolitan transportation policy making, and it can be adaptable in the metropolitan areas. Purpose of the study is to develope a model to help policy decision making for metropolitan transportation by setting policy scenarios of land use and transportation integration.