A conserved splicing silencer dynamically regulates O-GlcNAc transferase intron retention and O-GlcNAc homeostasis

Sung-Kyun Park,Xiaorong Zhou,Kathryn E. Pendleton,Olga V. Hunter,Jennifer J. Kohler,Kathryn A. O’Donnell,Nicholas K. Conrad 한국당과학회 2018 한국당과학회 학술대회 Vol.2018 No.01

Modification of nucleocytoplasmic proteins with O-GlcNAc regulates a wide variety of cellular processes and has been linked to human diseases. The enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) add and remove O-GlcNAc, but the mechanisms regulating their expression remain unclear. Here, we demonstrate that retention of the fourth intron of OGT is regulated in response to O-GlcNAc levels. We further define a conserved intronic splicing silencer (ISS) that is necessary for OGT intron retention. Deletion of the ISS in colon cancer cells leads to increases in OGT, but O-GlcNAc homeostasis is maintained by concomitant increases in OGA protein. However, the ISS-deleted cells are hypersensitive to OGA inhibition in culture and in soft agar. Moreover, growth of xenograft tumors from ISS-deleted cells is compromised in mice treated with an OGA inhibitor. Thus, ISS-mediated regulation of OGT intron retention is a key component in OGT expression and maintaining O-GlcNAc homeostasis.

광탄성실험 Hybrid 법에 의한 일정한 변형율을 받는 O-Ring 의 응력해석

권오성(O-Sung Kwon),황재석(Jai-Sug Hawong),남정환(Jeong-Hwan Nam),한송령(Song-Ling Han),박성한(Sung-Han Park) 대한기계학회 2006 대한기계학회 춘추학술대회 Vol.2006 No.11

In this research, stresses of O-ring with uniform deformation ratio in upper and lower direction are analyzed by photoelastic experimental hybrid method. The O-ring is made from rubber and is used to sealing of the high pressure vessel. The compressive deformation ratios of O-ring are 10% and 20%. Stress components (σ<SUB>xx</SUB>, σ<SUB>yy</SUB>, τ<SUB>xy</SUB>) of O-ring uniform ratios obtained from the photoelastic experimental hybrid method are almost identical to those from Hertz’s theory. Therefore, stress freezing method and photoelastic experimental hybrid method are effective applied to the stress analysis of O-ring made from rubber and with uniform ratios. The loading device developed in this research is used to produce the uniform deformation ratio of O-ring and as loading device of stress freezing. Stress distributions results from the upper contact surface of O-ring are almost identical to those from the lower contact surface of O-ring without regard to squeeze deformation ratio.

Pyrosequencing analysis of microbial communities in hollow fiber-membrane biofilm reactors system for treating high-strength nitrogen wastewater

Park, J.H.,Choi, O.,Lee, T.H.,Kim, H.,Sang, B.I. Pergamon Press 2016 CHEMOSPHERE - Vol.163 No.-

Wastewaters from swine farms, nitrogen-dealing industries or side-stream processes of a wastewater treatment plant (e.g., anaerobic digesters, sludge thickening processes, etc.) are characterized by low C/N ratios and not easily treatable. In this study, a hollow fiber-membrane biofilm reactors (HF-MBfR) system consisting of an O<SUB>2</SUB>-based HF-MBfR and an H<SUB>2</SUB>-based HF-MBfR was applied for treating high-strength wastewater. The reactors were continuously operated with low supply of O<SUB>2</SUB> and H<SUB>2</SUB> and without any supply of organic carbon for 250 d. Gradual increase of ammonium and nitrate concentration in the influent showed stable and high nitrogen removal efficiency, and the maximum ammonium and nitrate removal rates were 0.48 kg NH<SUB>4</SUB><SUP>+</SUP>-N m<SUP>-3</SUP> d<SUP>-1</SUP> and 0.55 kg NO<SUB>3</SUB><SUP>-</SUP>-N m<SUP>-3</SUP> d<SUP>-1</SUP>, respectively. The analysis of the microbial communities using pyrosequencing analysis indicated that Nitrosospira multiformis, ammonium-oxidizing bacteria, and Nitrobacter winogradskyi and Nitrobacter vulgaris, nitrite-oxidizing bacteria were highly enriched in the O<SUB>2</SUB>-based HF-MBfR. In the H<SUB>2</SUB>-based HF-MBfR, hydrogenotrophic denitrifying bacteria belonging to the family of Thiobacillus and Comamonadaceae were initially dominant, but were replaced to heterotrophic denitrifiers belonging to Rhodocyclaceae and Rhodobacteraceae utilizing by-products induced from autotrophic denitrifying bacteria. The pyrosequencing analysis of microbial communities indicates that the autotrophic HF-MBfRs system well developed autotrophic nitrifying and denitrifying bacteria within a relatively short period to accomplish almost complete nitrogen removal.

<tex> ${\rm TmBa}_{2}{\rm Cu}_{3}{\rm O}_{7-{\rm x}}$</tex> Superconducting Thin Films Grown by Pulsed Laser Deposition

Rock-Kil Ko,O-Jong Kwon,Kyu-Jeong Song,Ahmad, D.,Chan Park,Joon-Ho Lee,Seung-Su An,Nam-Jin Lee,Sang-Soo Oh,Young-Cheol Kim Institute of Electrical and Electronics Engineers 2007 IEEE transactions on applied superconductivity Vol.17 No.2

<P>TmBa<SUB>2</SUB>Cu<SUB>3</SUB>O<SUB>7</SUB> <SUB>-</SUB> <SUB>x</SUB> films which is one of the (Rare earth)Ba<SUB>2</SUB>Cu<SUB>3</SUB>O<SUB>7</SUB> <SUB>-</SUB> <SUB>x</SUB> high temperature superconductors with small ionic radius of rare earth element, were deposited on STO (100) single crystal substrates and Y<SUB>2</SUB>O<SUB>3</SUB>/YSZ/CeO<SUB>2</SUB> buffered metal substrates by pulsed laser deposition. The J<SUB>c</SUB> (77 K) and the value of onset of Tc TmBCO thin film on STO substrate was 4.5 MA/cm<SUP>2</SUP> and 86 K, respectively. The of J<SUB>c</SUB> TmBCO coated conductor on metal substrate was 1 MA/cm<SUP>2</SUP> . In-field property measurement was carried out at temperatures between 10 K and 77 K in magnetic filed up to 6 T in PPMS system. This is the first report, to the best of our knowledge, of TmBCO thin films and coated conductors with TmBCO film as the superconducting layer which have critical current densities at 77 K of 4.5 MA/ cm<SUP>2</SUP> and 1 MA/cm<SUP>2</SUP> , respectively. The superconducting properties of TmBCO films and the possibility of using TmBCO film as the superconducting layer of the HTS coated conductor, were investigated.</P>

Distinct hydrophobic–hydrophilic dual interactions occurring in the clathrate hydrates of 3,3-dimethyl-1-butanol with help gases

Moon, Seokyoon,Park, Sung O.,Ahn, Yun-Ho,Kim, Heejoong,Shin, Eunhye,Hong, Sujin,Lee, Yunseok,Kwak, Sang Kyu,Park, Youngjune Elsevier 2018 CHEMICAL ENGINEERING JOURNAL -LAUSANNE- Vol.348 No.-

<P><B>Abstract</B></P> <P>To unlock the potential of clathrates hydrate for versatile applications in energy and environmental application such as energy storage, gas separation, and novel functional materials, profound understanding of their hidden nature must be secured. In this study, we focused on the complex host–guest and heterogeneous guest–guest interactions occurring on the sH hydrates of 3,3-dimethyl-1-butanol with help gases of CH<SUB>4</SUB> or CO<SUB>2</SUB>. The density functional theory calculations and spectroscopic experimental analyses showed that the dynamics of the large 3,3-dimethyl-1-butanol guest molecule as well as the host water frameworks of sH hydrate were significantly influenced by the type of gaseous co-guest molecules via complex host–guest and/or guest–guest interactions. The flexible hydrogen-bonded water framework underwent contraction or elongation in the O:H–O hydrogen and O–H polar-covalent bonds induced by distinct occupation patterns of the co-guest help gases, and it was observed that, depending on the type, the co-guest help gases triggered the transformation of torsional configuration of the hydrophobic moieties of the large 3,3-dimethyl-1-butanol guest molecule while the hydrophilic part was incorporated into the host water framework.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The complex host–guest interactions occurring on sH hydrates was investigated <I>via</I> experimental and DFT calculation. </LI> <LI> The dynamics of DMB guest molecules were significantly influenced by the type of gaseous co-guest molecules. </LI> <LI> The contraction and/or elongation patterns of the intermolecular hydrogen bond were examined. </LI> <LI> The co-guest help gases evoked a discrete torsional configuration of the DMB molecule. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

CHF₃ / C₂F6 플라즈마에 의한 실리콘 표면 잔류막의 특성

권광호(K.-H. Kwon),박형호(H.-H. Park),이수민(S. M. Lee),강성준(S. J. Kang),권오준(O.-J. Kwon),김보우(B.W. Kim),성영권(Y.-K. Sung) 한국진공학회(ASCT) 1992 Applied Science and Convergence Technology Vol.1 No.1

실리콘을 CHF₃/C₂F_6 가스 플라즈마를 이용하여 식각하면 실리콘위에 탄소, 불소 및 산소로 이루어진 잔류막이 형성된다. 이 잔류막을 XPS로 분석한 결과 탄소는 C-Si, C-Si, C-C/H, C-CF_x(x≤3), C-F, C-F₂, C-F₃ 결합을 하고 있으며, 불소는 F-Si, F-C 및 F-O 결합으로 이루어져 있음을 알았다. 한편 산소는 O-Si 및 O-F 결합으로, 실리콘은 Si-Si, Si-C 및 Si-O 결합상태를 나타낸다. 잔류막의 수직분포 연구를 통하여 Si-O 및 Si-C 결합이 탄소와 불소의 결합층 아래에 존재하고, 잔류막의 표면부에 F-O 결합이 분포함을 알았다. 또한 건식식각 변수가 잔류막 형성에 미치는 영향이 조사되었으며 CHF₃/C₂F_6 가스 유량비, RF power 벚 압력 등이 잔류막의 두께, 조성비 및 잔류막의 결합상태에 영향을 미침을 알 수 있었다. Si surfaces exposed to CHF₃/C₂F_6 gas plasmas in reactive ion etching (RIE) have been characterized by X-ray photoelectron spectroscopy (XPS). CHF₃/C₂F_6 gas plasma exposure of Si surface leads to the deposition of residual film containing carbon and fluorine. The narrow scan spectra of C 1s show various bonding states of carbon as C-Si, C-Si, C-C/H, C-CF_x(x≤3), C-F, C-F₂, and C-F₃. The chemical bonding states of fluorine are described with F-Si, F-C, and F-O. And the oxygen and silicon are also detected. The effects of parameters for reactive ion etching as CHF₃/C₂F_6 gas ratio, RF power, and pressure are investigated.

<i>Deqi</i> Induction by HT7 Acupuncture Alters Theta and Alpha Band Coherence in Human Healthy Subjects

Lee, Go-Eun,Yun, Jong-Min,Yang, Seung-Bum,Kang, Yeonseok,Kang, Hyung-Won,Choi, Kwang-Ho,Kim, Junbeom,Kwon, O. Sang,Park, Ji-Eun,Kim, Jae-Hyo Hindawi 2017 Evidence-based Complementary and Alternative Medic Vol.2017 No.-

<P>The aim of this preliminary study is to investigate the changes in phase synchronization in the theta and alpha bands before and during the performance of classical acupuncture on the Sinmun (HT7). The electroencephalogram (EEG) signals from nine healthy young subjects were recorded before and during acupuncture in the “closed-eye” state. The EEG signals were acquired from 19 surface scalp electrodes (FP1, FP2, F7, F3, Fz F4, F8, T3, C3, Cz, C4, T4, T5, P3, Pz, P4, T6, O1, and O2). Needles were inserted into the HT7 bilaterally and were then manipulated to induce<I> deqi</I> and retained for 15 minutes. Phase synchronization was measured by phase coherence. In the theta band, coherence significantly increased between the temporal (T5, T6) and occipital areas (O1, O2) during the acupuncture stimulation. In the alpha band, coherence significantly increased between the left temporal area (T5) and other areas (frontal, parietal, and occipital). Phase coherence in the theta and alpha bands tended to increase during the retention of the acupuncture needles after<I> deqi</I>. Therefore, it can be concluded that acupuncture stimulation with<I> deqi</I> is clinically effective via the central nervous system (CNS).</P>

$\textrm{Al}_2\textrm{O}_3$를 함유하는 AIN세라믹스의 반응소결 및 열전도도

김영우,이윤복,박상희,오기동,박홍채,Kim, Yeong-U,Lee, Yun-Bok,Park, Sang-Hui,O, Gi-Dong,Park, Hong-Chae 한국재료학회 1998 한국재료학회지 Vol.8 No.1

5-64.3mol% AI$_{2}$O$_{3}$를 함유하는 AIN(1wt% $Y_{2}$O$_{3}$)의 1650-190$0^{\circ}C$ 상압소결에 따른 치밀화 거동, 미세구조, 열전도도가 검토 되었다. XRD 분석결과, AION(5NIN \ulcorner9 AI$_{2}$O$_{3}$ ), 27R AIN다형, AIN이 소결체의 주상으로서 동정되었다. AI$_{2}$O$_{3}$ 의 함량이 증가할수록 소결체의 부피밀도는 증가 하였다. AION을 기지상으로 하는 물질($\geq$ 30mol% AI$_{2}$O$_{3}$ )인 경우는 175$0^{\circ}C$ 소결에서 최대의 부피밀도를 나타내었으며, AIN을 기지상으로 하는 경우(5mol% AI$_{2}$O$_{3}$ ) 는 소결온도가 증가할수록 밀도가 감소하였다. $Y_{2}$O$_{3}$의 존재하에서 주로 185$0^{\circ}C$이상에서 AI$_{2}$O$_{3}$ 와 AIN의 반응에 으해서 액상이 생성되었다. AION을 기지로 하는 물질의 치밀화는 주로 액상의생성 및 AION의 입성장에 의해서 지배되었으나, AIN을 기지로 하는 물질에 있어서는 1$650^{\circ}C$에서 액상이 생성되었고, 소결온도가 190$0^{\circ}C$까지 상승할 동안 AIN의 입성장은 크게 일어나지 않았다. AI$_{2}$O$_{3}$ 함량이 증가할수록 낮은 열도도를 갖는 다량의 AION 및 액상의 생성으로 인하여 소결체의열전도도는 감소 하였다. 5mol% AI$_{2}$O$_{3}$ 를 함유한 190$0^{\circ}C$ 소결체가 최대의 열전도도(77.9W/(m\ulcornerk))를 나타내었다.

질화규소의 기계적 특성에 미치는 미세구조 및 소결조제의 영향

박혜련,이오상,박희동,이재도,Park, Hye-Ryeon,Lee, O-Sang,Park, Hui-Dong,Lee, Jae-Do 한국재료학회 1992 한국재료학회지 Vol.2 No.5

Four distinctive hot pressed and heat treated S${i_3}{N_4}$ceramics, S${i_3}{N_4}$-8%${Y_2}{O_3}$, S${i_3}{N_4}$-6% ${Y_2}{O_3}$-2% $A{l_2}{O_3}$, S${i_3}{N_4}$-4% ${Y_2}{O_3}$-3% $A{l_2}{O_3}$, 그리고 S${i_3}{N_4}$-1% MgO-1% Si$O_2$(in wt%), were prepared and characterized by X-ray diffraction, scanning electron microscopy, image analysis and mechanical tests. The fracture toughness of S${i_3}{N_4}$-8% ${Y_2}{O_3}$specimens containing large elongated grains showed the highest value of about 9.8MPa$m^{1/2}$. Two out of four S${i_3}{N_4}$, ceramics(S${i_3}{N_4}$-6% ${Y_2}{O_3}$-2% $A{l_2}{O_3}$and S${i_3}{N_4}$-4% ${Y_2}{O_3}$-3% $A{l_2}{O_3}$) heat treated at 200 $0^{\circ}C$retained the fracture strength of over 900MPa and fracture toughness of over 8.0MPa$m^{1/2}$. Large ${\beta}$-S${i_3}{N_4}$grains having a diameter larger than 1${\mu}$m appeared to contribute to increase in fracture toughness. 질화규소 소재로 S${i_3}{N_4}$-8%${Y_2}{O_3}$, S${i_3}{N_4}$-6% ${Y_2}{O_3}$-2% $A{l_2}{O_3}$, S${i_3}{N_4}$-4% ${Y_2}{O_3}$-3% $A{l_2}{O_3}$, 그리고 S${i_3}{N_4}$-1% MgO-1% Si$O_2$의 4가지 조성을 선정하여 주상모양의 ${\beta}$-S${i_3}{N_4}$결정상을 성장시켰으며, XRD, SEM, 입도분석과 기계적 특성을 조사하였다. S${i_3}{N_4}$-8% ${Y_2}{O_3}$에서는 파괴인성값을 9.8MPa$m^{1/2}$까지 얻었으며 200$0^{\circ}C$에서 열처리한 후에도 파괴강도(>900MPa)의 감소없이 파괴인성이 8.0MPa$m^{1/2}$이상인 S${i_3}{N_4}$-6% ${Y_2}{O_3}$-2% ${l_2}{O_3}$, 와 S${i_3}{N_4}$-4% ${Y_2}{O_3}$-3% $A{A_2}{O_3}$를 얻었다. 질화규소의 파괴인성이 ${\beta}$-S${i_3}{N_4}$-결정상 크기의 증가에 따라 직선적으로 증가하는 관계를 갖는 파괴인성에 대한 미세구조의 영향을 고찰하였다.

Intra-Arterial Thrombolysis Using Double Devices: Mechanicomechanical or Chemicomechanical Techniques

Park, Hyun,Hwang, Gyo-Jun,Jin, Sung-Chul,Bang, Jae-Seung,Oh, Chang-Wan,Kwon, O-Ki The Korean Neurosurgical Society 2012 Journal of Korean neurosurgical society Vol.51 No.2

Objective : To optimize the recanalization of acute cerebral stroke that were not effectively resolved by conventional intraarterial thrombolysis (IAT), we designed a double device technique to allow for rapid and effective reopening. In this article, we describe the feasibility and efficacy of this technique. Methods : From January 2008 to September 2009, twenty patients with acute cerebral arterial occlusion (middle cerebral artery : n=12; internal carotid artery terminus : n=5; basilar artery : n=3) were treated by the double device technique. This technique was applied when conventional thrombolytic methods using drug, microwires, microcatheters and balloons did not result in recanalization. In the double device technique, two devices are simultaneously placed at the lesion (for example, one microcatheter and one balloon or two microcatheters). Chemicomechanical or mechanicomechanical thrombolysis was performed simultaneously using various combinations of two devices. Recanalization rates, procedural time, complications, and clinical outcomes were analyzed. Results : The initial median National Institute of Health Stroke Scale (NIHSS) was 16 (range 5-26). The double device technique was applied after conventional IAT methods failed. Recanalization was achieved in 18 patients (90%). Among them, 55% (11 cases) were complete (thrombolysis in cerebral infarction 2B, 3). The median thrombolytic procedural time including the conventional technique was $135{\pm}83.7$ minutes (range 75-427). Major symptomatic hemorrhages (neurological deterioration ${\geq}4$ points in NIHSS) developed in two patients (10%). Good long term outcomes (modified Rankin Scale ${\leq}2$ at 90 days) occurred in 25% (n=5) of the cases. Mortality within 90 days developed in two cases (10%). Conclusion : The double device technique is a feasible and effective technical option for large vessel occlusion refractory to conventional thrombolysis.