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Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects
Tsugunori Notomi,Yasuyoshi Mori,Norihiro Tomita,Hidetoshi Kanda 한국미생물학회 2015 The journal of microbiology Vol.53 No.1
Loop-mediated isothermal amplification (LAMP), a newlydeveloped gene amplification method, combines rapidity,simplicity, and high specificity. Several tests have been developedbased on this method, and simplicity is maintainedthroughout all steps, from extraction of nucleic acids to detectionof amplification. In the LAMP reaction, samples areamplified at a fixed temperature through a repetition of twotypes of elongation reactions occurring at the loop regions:self-elongation of templates from the stem loop structureformed at the 3 -terminal and the binding and elongation ofnew primers to the loop region. The LAMP reaction has awide range of possible applications, including point-of-caretesting, genetic testing in resource-poor settings (such as indeveloping countries), and rapid testing of food productsand environmental samples.