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In vitro cytotoxic and antioxidant activities of Pikut Trichinthalamaga remedy
Bhanuz Dechayont,Chayaporn Limpichai,Kornrawee Kornwisitwathin,Nitra Nuengchamnong,Arunporn Itharat 경희대학교 융합한의과학연구소 2017 Oriental Pharmacy and Experimental Medicine Vol.17 No.3
The Pikut Trichinthalamaga remedy appears in ancient Thai pharmacy scripture, as a purgative, and includes Rhubarb (Rheum palmatum L.), Myrabulan fruit (Terminalia chebula Retz.), and Gamboge (Garcinia handuryi Hook F.). We investigated the cytotoxic properties of the herbal extracts using the SRB assay in human colon adenocarcinoma cell lines (LS174T and SW480) and normal lung fibroblast cell (MRC-5) as well as their antioxidant activities by chemical-(ABTS assay) and cell-based assays [nitric oxide (NO) assay]. The active fractions were identified using high-performance liquid chromatography coupled to electrospray ionisation and quadruple time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS). The ethanolic extract of Pikut Trichinthalamaga remedy had a cytotoxic effect on LS174T and SW 480 cells (IC50 = 0.39 and 0.51 μg/ml, respectively). The ABTS assay showed that the water extract of the myrabulan fruit had the highest activity (EC50 = 5.69 μg/ml) while the NO assay showed that the ethanolic extract of Pikut Trichinthalamaga remedy had the most potent inhibitory activity on NO production (EC50 = 0.62 μg/ml). After liquid–liquid partitioning sequence, the chloroform fraction had the highest cytotoxicity. Emodin, forbesione, gaudichaudionic acid and gaudichaudionol were identified in this extract. These results support the further investigation of the Pikut Trichinthalamaga remedy as a potential anticancer drug.
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Acharaporn Duangjai,Nungruthai Suphrom,Jukkrit Wungrath,Atcharaporn Ontawong,Nitra Nuengchamnong,Atchariya Yosboonruang 한국한의학연구원 2016 Integrative Medicine Research Vol.5 No.4
Background This study explored the bioactivities and nutrient compositions of coffee (Coffea Arabica L.) pulp which was prepared in three different ways [Coffee Pulp Extracts (CPE) 1–3]. Methods The coffee pulp was prepared in three different ways by distinct selecting and freezing processes. The nutritional values, polyphenol contents, antioxidant activity, and antibacterial properties of the coffee pulp as well as the characterization of the active ingredients by liquid chromatography-electrospray ionization-quadrupole-time-of-flight mass spectrometry (LC-ESI-Q-TOF-MS) were evaluated. Results The chemical profiles of three aqueous extracts were compared and characterized using LC-ESI-QTOF-MS. They showed slightly different nutrient compositions. The total phenolic content was highest in CPE1, and decreased in the following order: CPE1 > CPE2 > CPE3. Among the CPEs tested, CPE1 showed the most potent antioxidant activity with IC50 18 μg/mL and 82 μg/mL by 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and 1,1-diphenyl-2-picryl-hydrazyl assay, respectively. Chlorogenic acid and caffeine were the most prominent in CPE1 and it contained more compounds than the others. Moreover, CPE1 demonstrated antibacterial activity against both gram-positive (Staphylococcus aureus and Staphylococcus epidermidis) and gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli). Conclusion These findings indicated that CPE1 has powerful nutrients with antioxidant and antibacterial properties—the potency of which is impacted by the preparation process.
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