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A transcriptional atlas of the silk gland in Antheraea pernyi revealed by IsoSeq
Duan Jianping,Li Shanshan,Zhang Zhengtian,Yao Lunguang,Yang Xinfeng,Ma Sanyuan,Duan Nini,Wang Jiazhen,Zhu Xuwei,Zhao Ping 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2
Silk fibers spun by the silk gland of Antheraea pernyi have many unique properties and are of great value in genetic improvement and non-traditional applications. However, the complete transcriptional landscape and accurate genic annotation of the silk gland are yet to be conducted, which limits related studies on this organ. In this study, isoform sequencing revealed the full-length transcriptome of the A. pernyi silk gland, producing 12,572 high-confidence isoforms from 7,658 genes, among which more than 40 % of genes have not yet been annotated in the reference genome. Moreover, approximately 9 % of isoforms are computationally identified as long non-coding RNAs (lncRNAs). Up to 1,492 alternative splicing (AS) and 3,068 alternative polyadenylation (APA) events were revealed within this transcriptome. In addition, 2,569 putative transcription factors (TFs) belonging to 68 different families were first identified in A. pernyi genome, including 871 TFs in silk gland, and some TF families have undergone expansion or contraction. This study significantly improve our knowledge of the genes expressed in the silk gland of A. pernyi and provide a valuable resource for the in-depth study of silk protein synthesis and spinning, genetic improvement, and non-traditional applications in A. pernyi.
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Lingling Xu,Jie Wu,Nini Li,Chengjun Jiang,Yan Guo,Peng Cao,Dianlei Wang 대한생리학회-대한약리학회 2020 The Korean Journal of Physiology & Pharmacology Vol.24 No.6
The present study aimed to examine the effect of allyl isothiocyanate (AITC) on chronic obstructive pulmonary disease and to investigate whether upregulation of multidrug resistance-associated protein 1 (MRP1) associated with the activation of the PARK7 (DJ-1)/nuclear factor erythroid 2-related factor 2 (Nrf2) axis. Lung function indexes and histopathological changes in mice were assessed by lung function detection and H&E staining. The expression levels of Nrf2, MRP1, heme oxygenase-1 (HO-1), and DJ-1 were determined by immunohistochemistry, Western blotting and reverse transcription-quantitative polymerase chain reaction. Next, the expression of DJ-1 in human bronchial epithelial (16HBE) cells was silenced by siRNA, and the effect of DJ-1 expression level on cigarette smoke extract (CSE)-stimulated protein degradation and AITC-induced protein expression was examined. The expression of DJ-1, Nrf2, HO-1, and MRP1 was significantly decreased in the wild type model group, while the expression of each protein was significantly increased after administration of AITC. Silencing the expression of DJ-1 in 16HBE cells accelerated CSE-induced protein degradation, and significantly attenuated the AITC-induced mRNA and protein expression of Nrf2 and MRP1. The present study describes a novel mechanism by which AITC induces MRP1 expression by protecting against CS/CSEmediated DJ-1 protein degradation via activation of the DJ-1/Nrf2 axis.
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Guihong Zhao,Tao Li,Xinyun Qu,Nini Zhang,Miao Lu,Jing Wang 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.5
An effective method for the ultrasound-assisted extraction of indigo and indirubin from Isatis indigotica Fort. was established and their antioxidant activities were investigated. Response surface methodology based on a three-level, four-factor Box–Behnken design was used to optimize the extraction conditions. Analysis of variance showed that the quadratic model was significant for the extraction of indigo and indirubin (112.72% ± 1.65% and 116.42% ± 1.27%, respectively) under the optimal conditions (methanol concentration, 80%; extraction time, 25 min; ratio of solid to liquid, 1:34 g/mL; and extraction temperature, 41 C) and was in good agreement with the predicted value. Moreover, evaluation of the antioxidant activities suggested that indigo and indirubin presented better scavenging effects on 1,1-diphenyl-2-picrylhydrazyl free radical and superoxide radical than the extract and the extract revealed certain antioxidant activities in hydroxyl radical scavenging and reducing power, and indigo and indirubin could be used as natural antioxidants in the food or medicine industry.
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Yujie Zhang,Xu Zhang,Nini Cheng,Yanping Li,Jinyi Xing 한국유전학회 2015 Genes & Genomics Vol.37 No.7
Identification of essential fragments in the promoter region of genes to drive tissue-specific expression of exogenous genes in the skeletal muscle is obligatory for animal transgenic study. The skeletal a-actin is a major protein of the thin filaments of skeletal muscle fiber. In this study, the specificity and activity of porcine skeletal aactin gene promoter were investigated by approaches of cell transfection and assayed with green fluorescent protein (GFP) and dual luciferase reporter activity, respectively. The results revealed that the obtained 3717 bp fragment of porcine skeletal a-actin gene promoter drives GFP to be expressed uniquely in murine C2C12 cells, and two segments of 2.0–2.3 and 0.06–0.37 kb in the promoter region were essential for porcine skeletal a-actin gene expression. The regulatory elements in the 0.06–0.37 kb fragment were further investigated, and when it is deleted or the CArG box within this fragment was mutated, the promoter activity was reduced to 20 or 43 % (P\0.01), respectively, compared to the 3.04 kb segment, suggesting an important role of CArG box in regulating porcine skeletal a-actin gene transcription. The results may provide reference for creating transgenic pigs to express exogenous genes uniquely in pork.
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