Preclinical Study of Cell Therapy for Osteonecrosis of the Femoral Head with Allogenic Peripheral Blood-Derived Mesenchymal Stem Cells

Tao Zhang,Qiang Fu,Ning-Ning Tang,Qian Zhang,Yi Liu,Jia-Chen Peng,Ning Fang,Li-Mei Yu,Jin-Wei Liu 연세대학교의과대학 2016 Yonsei medical journal Vol.57 No.4

Purpose: To explore the value of transplanting peripheral blood-derived mesenchymal stem cells from allogenic rabbits (rPBMSCs)to treat osteonecrosis of the femoral head (ONFH). Materials and Methods: rPBMSCs were separated/cultured from peripheral blood after granulocyte colony-stimulating factor mobilization. Afterwards, mobilized rPBMSCs from a second passage labeled with PKH26 were transplanted into rabbit ONFH models, which were established by liquid nitrogen freezing, to observe the effect of rPBMSCs on ONFH repair. Then, the mRNA expressions of BMP-2 and PPAR-γ in the femoral head were assessed by RT-PCR. Results: After mobilization, the cultured rPBMSCs expressed mesenchymal markers of CD90, CD44, CD29, and CD105, but failed to express CD45, CD14, and CD34. The colony forming efficiency of mobilized rPBMSCs ranged from 2.8 to 10.8 per million peripheralmononuclear cells. After local transplantation, survival of the engrafted cells reached at least 8 weeks. Therein, BMP-2 was up-regulated, while PPAR-γ mRNA was down-regulated. Additionally, bone density and bone trabeculae tended to increase gradually. Conclusion: We confirmed that local transplantation of rPBMSCs benefits ONFH treatment and that the beneficial effects are relatedto the up-regulation of BMP-2 expression and the down-regulation of PPAR-γ expression.

Blind Separation of Permuted Alias Image with Motion Blurred Using Image Enhancement in NSCT Domain

DUAN Xin-tao,WANG Jing-juan,PENG Tao,LI Fei-fei,LIU Shang-Wang,LIU Tuan-ning 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.3

Focused on the issue that motion blurred permuted alias image blind separation, an algorithm using image enhancement based on nonsubsampled contourlet transform (NSCT) domain was proposed. Firstly, permuted alias image was decomposed into low-frequency sub-band and high-frequency sub-bands, which were obtained by spare decomposition based on NSCT domain. Coefficients of high-frequency sub-bands were enhanced according to Bayesian shrinkage threshold and nonlinear gain function, and the enhanced version was got by this method. Then the permuted alias image and the enhanced version were blocked, the correlation coefficients were estimated by each corresponding sub-block, because the permuting image was changed larger, the permuting image could be separated by using threshold method. Experimental results show that the proposed algorithm can separate the permuting image effectively from the permuted alias image in spite of the motion blurred direction, blur degree, size, location and the number of permuting image.

Antiproliferative activities of Garcinia bracteata extract and its active ingredient, isobractatin, against human tumor cell lines

Tao Shen,Wei Li,Yan-Yan Wang,Qing-Qing Zhong,Shu-Qi Wang,Xiao-Ning Wang,Dong-Mei Ren,Hongxiang Lou 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.3

In our cell based screening of antitumoringredients from plants, the EtOH extract of Garciniabracteata displayed antiproliferative effect against humanlung adenocarcinoma A549 cells, human breast cancerMCF-7 cells, and human prostate cancer PC3 cells. Phytochemicalinvestigation of this active extract producednine ingredients, and their structures were established byanalysis of MS and NMR spectra. Antiproliferative evaluationof isolated ingredients on A549, MCF-7 and PC3cells indicated that a xanthone named isobractatin (1)exhibited potent antiproliferative activity against the abovethree human cancer cell lines with IC50 values rangingfrom 2.90 to 4.15 lM. Treatment of PC3 cells with 1 led toan enhancement of the cell apoptosis, and arrested cellcycle in the G0/G1 phase. The G0/G1 phase cycle-relatedproteins analysis showed that the expressions of cyclins D1and E were reduced by 1, whereas the protein level of cyclin dependent kinase (CDK) inhibitor P21 was induced. Additionally, 1 enhanced PC3 cell apoptosis by activationsof Bax, caspases 3 and 9, and by inhibition of Bcl-2. Ourcombined data illustrated that isobractatin (1) was theantiproliferative ingredient of G. bracteata against threehuman cancer cell lines, which exerted its antiproliferatriveeffect via cell cycle arrest and induction of apoptosis.

Sex pheromone of the jumping plant bug, Halticus minutus Reuter (Hemiptera: Miridae)

Tao Zhang,Xiaofang Zhang,Xiangdong Mei,Yaofa Li,Zhanlin Gao,Jun Ning 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2

The jumping plant bug, Halticusminutus Reuter, is a pest of sweet potatoes, soybeans and turfgrass in China. Here we present our analysis of the female-produced sex pheromone. These plant bugs release their sex pheromone, consisting hexyl acetate (HA), hexyl butyrate (HB) and (E)-4-oxo-2-hexenal (4-OHE), at night. These three components, tested individually, elicited strong antennal responses. Field tests indicated that a ternary blend of HA, HB and 4-OHE, at ratios of 1:16:10, effectively attractedmale H. minutus. Our dose-response analysis showed optimal male capture at the trap dose of 13.5mg/trap. Higher trap doses did not improve capture rates. Higher ratios of HB in the pheromone mix led to substantially reduced captures, fromwhich we infer that HBmay function as an alarm pheromone or defensive secretion. Pipette tips were used as long-term lures, and whose longevities were evaluated. Results showed effective duration of pipette tip lures were N5 weeks. Thus, we suggest that the pheromone-baited traps are economically viable tools for long-term monitoring and trapping of jumping plant bugs.

In Vitro and In Vivo Study on the Effect of Lysosome-associated Protein Transmembrane 4 Beta on the Progression of Breast Cancer

Deyou Tao,Junqing Liang,Yihong Pan,Yanting Zhou,Ying Feng,Lin Zhang,Jingjing Xu,Hui Wang,Ping He,Jie Yao,Yang Zhao,Qinjie Ning,Wen Wang,Wei Jiang,Jing Zheng,Xia Wu 한국유방암학회 2019 Journal of breast cancer Vol.22 No.3

Purpose: Although the effect of lysosome-associated protein transmembrane 4 beta (LAPTM4B) on the proliferation, migration, and invasion of breast cancer (BC) cells has already been studied, its specific role in BC progression is still elusive. Here, we evaluated the effect of different levels of LAPTM4B expression on the proliferation, invasion, adhesion, and tumor formation abilities of BC cells in vitro, as well as on breast tumor progression in vivo. Methods: We investigated the influence of LAPTM4B expression on MCF-7 cell proliferation, invasion, adhesion, and tube formation abilities in vitro through its overexpression or knockdown and on breast tumor progression in vivo. Results: Cell growth curves and colony formation assays showed that LAPTM4B promoted the proliferation of breast tumor cells. Cell cycle analysis results revealed that LAPTM4B promoted the entry of cells from the G1 into the S phase. Transwell invasion and cell extracellular matrix adhesion assays showed that LAPTM4B overexpression increased the invasion and adhesion capabilities of MCF-7 cells. More branches were observed in MCF-7 cells overexpressing LAPTM4B under an electron microscope. In comparison with LAPTM4B overexpression, LAPTM4B knockdown decreased the expression of vascular endothelial growth factor-A and significantly inhibited the vasculogenic tube formation ability of tumors. These results were also verified with western blot analysis. Conclusion: LAPTM4B promoted the proliferation of MCF-7 cells through the downregulation of p21 (WAF1/CIP1) and caspase-3, and induced cell invasion, adhesion, and angiogenesis through the upregulation of hypoxia-inducible factor 1 alpha, matrix metalloproteinase 2 (MMP2), and MMP9 expression. This specific role deems LAPTM4B as a potential therapeutic target for BC treatment.

Optical Super-Resolution Imaging of Surface Reactions

Chen, Tao,Dong, Bin,Chen, Kuangcai,Zhao, Fei,Cheng, Xiaodong,Ma, Changbei,Lee, Seungah,Zhang, Peng,Kang, Seong Ho,Ha, Ji Won,Xu, Weilin,Fang, Ning American Chemical Society 2017 Chemical reviews Vol.117 No.11

<P>Optical super-resolution imaging has gained momentum in investigations of heterogeneous and homogeneous chemical reactions at the single-molecule level. Thanks to its exceptional spatial resolution and ability to monitor dynamic systems, much detailed information on single-molecule reaction/adsorption processes and single-particle catalytic processes has been revealed, including chemical kinetics and reaction dynamics; active-site distributions on single-particle surfaces; and size-, shape-, and facet-dependent catalytic activities of individual nanocatalysts. In this review, we provide an overview of recent advances in super-resolution chemical imaging of surface reactions.</P>

S-I model of horizontal jet grouting reinforcement for soft soil

Zhang, Ning,Li, Zhongyin,Ma, Qingsong,Ma, Tianchi,Niu, Xiaodong,Liu, Xixi,Feng, Tao Techno-Press 2018 Geomechanics & engineering Vol.15 No.5

A superposition-iteration (S-I) model is proposed to simulate the jet grouting pre-reinforcing impact for a shallow-buried tunnel. The common model is deduced by theoretical (force equilibrium) analysis and then transformed into the numerical formulation. After applying it to an actual engineering problem, the most obvious deficiency was found to be continuous error accumulation, even when the parameters change slightly. In order to address this problem, a superposition-iteration model is developed based on the basic assumption and superposition theory. First, the additional deflection between two successive excavation steps is determined. This is caused by the disappearance of the supporting force in the excavated zone and the soil pressure in the disturbed zone. Consequently, the final deflection can be obtained by repeatedly superposing the additional deflection to the initial deflection in the previous steps. The analytical solution is then determined with the boundary conditions. The superposition-iteration model is thus established. This model was then applied and found to be suitable for real-life engineering applications. During the calculation, the error induced by the ill-conditioned problem of the matrix is easily addressed. The precision of this model is greater compared to previous models. The sensitivity factors and their impact are determined through this superposition-iteration model.

An Improved Mathematical Model for Estimating Polarization Parameters of Transformer Oil-Paper Insulation

Zhang Tao,Mandala Antonio Tome,Zuo Qian,Zhang Ning,Jiang Shijie 대한전기학회 2022 Journal of Electrical Engineering & Technology Vol.17 No.4

The parameters of dielectric response based on extended Debye circuit model can refl ect oil-paper insulation state. The model using the initial slop of recovery voltage to estimate circuit parameters, serve as good quality consistency with the measured data. However, the model can be applicable for large power transformers but not for distribution transformers. This paper proposed an improved mathematical model using recovery voltage peak, peak time and initial slope characteristics to solve dielectric response equivalent circuit parameters. The identifi cation of equivalent circuit parameters is converted into a mathematical optimization problem. And then particle swarm optimization algorithm is used for solving the problem. The improved mathematical model can decrease sampling data of recovery voltage. To check the validity of the estimated parameters, the on-site measured data of RVM experiments on actual transformers in various capacities is applied. The calculated result shows that the recovery voltage curve calculated and measured recovery voltage curve have good consistency, which can be advantageous to diagnose the oil-paper insulation status of transformer. It illustrates that the improved method proposed in this paper is feasible and eff ective.

Antiproliferative activities of Garcinia bracteata extract and its active ingredient, isobractatin, against human tumor cell lines

Shen, Tao,Li, Wei,Wang, Yan-Yan,Zhong, Qing-Qing,Wang, Shu-Qi,Wang, Xiao-Ning,Ren, Dong-Mei,Lou, Hong-Xiang 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.3

In our cell based screening of antitumor ingredients from plants, the EtOH extract of Garcinia bracteata displayed antiproliferative effect against human lung adenocarcinoma A549 cells, human breast cancer MCF-7 cells, and human prostate cancer PC3 cells. Phytochemical investigation of this active extract produced nine ingredients, and their structures were established by analysis of MS and NMR spectra. Antiproliferative evaluation of isolated ingredients on A549, MCF-7 and PC3 cells indicated that a xanthone named isobractatin (1) exhibited potent antiproliferative activity against the above three human cancer cell lines with $IC_{50}$ values ranging from 2.90 to $4.15{\mu}M$. Treatment of PC3 cells with 1 led to an enhancement of the cell apoptosis, and arrested cell cycle in the G0/G1 phase. The G0/G1 phase cycle-related proteins analysis showed that the expressions of cyclins D1 and E were reduced by 1, whereas the protein level of cyclin dependent kinase (CDK) inhibitor P21 was induced. Additionally, 1 enhanced PC3 cell apoptosis by activations of Bax, caspases 3 and 9, and by inhibition of Bcl-2. Our combined data illustrated that isobractatin (1) was the antiproliferative ingredient of G. bracteata against three human cancer cell lines, which exerted its antiproliferatrive effect via cell cycle arrest and induction of apoptosis.

Molecular cloning and characterization of SoNCED, a novel gene encoding 9-cis-epoxycarotenoid dioxygenase from sugarcane (Saccharum officinarum L.)

Chang-Ning Li,Manoj-Kumar Srivastava,Qian Nong,Li-Tao Yang,Yang-Rui Li,디조이사마하나마 한국유전학회 2013 Genes & Genomics Vol.35 No.1

Abscisic acid (ABA) plays important roles in adaptive responses to various environmental stresses. The rate-limiting step in ABA biosynthesis is the oxidative cleavage of cis-epoxycarotenoids, which is catalyzed by 9-cis-epoxycarotenoid dioxygenase (NCED). In this experiment,a full-length cDNA encoding NCED gene was cloned by RT-PCR and RACE from sugarcane (Saccharum officinarum L.). The full-length of SoNCED is 2,521 bp with 1,827 bp open reading frame, encoding a peptide of 608amino acids. The calculated molecular weight of protein was 65.9 kDa with isoelectric point of 6.04. Conserved domains prediction indicated a chloroplast-targeting peptide located at N-terminus of SoNCED. Phylogenetic tree, constructed by Neighbor-Joining method indicated that SoNCED shared high identity with the NCEDs reported from other plant species. Sequence alignment revealed that the basic secondary structure including a-helices, b-strands, b-propeller and His residues coordinating catalytic sites of SoNCED were highly conserved as in the NCEDs from other plants. Tissue specific expression analysis using quantitative real-time PCR showed a significant increase in SoNCEDmRNAlevel and its correlation with O2– production rate and ABA accumulation in leaves and roots of sugarcane variety GT21 when exposed to water stress. Further, the stimulation of SoNCED mRNA level, O2– production rate and ABA content after exogenous application of ABA (100 lMol l-1) proved its involvement in pathways providing tolerance to drought stress.