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      • Vietnamese Version of Pittsburgh Sleep Quality Index: Reliability, Cut-Off Point, and Association With Depression Among Health Science Students

        Nguyen An Huynh Bao,Nguyen Ngoc Thi Tieu,Vo Nghia Quang,Nguyen Dung Hoang 대한수면학회 2024 sleep medicine research Vol.15 No.1

        Background and Objective Sleep disturbances among medical students, including insufficient sleep duration and poor sleep quality, are prevalent issues with potential repercussions on mental health.Methods The study evaluated the Vietnamese version of the Pittsburgh Sleep Quality Index (PSQI) to assess sleep quality and investigated the correlation between the results of the PSQI and Beck Depression Inventory-II (BDI-II) scales in health sciences students.Results We conducted this cross-sectional study at the School of Medicine, Vietnam National University Ho Chi Minh City, involving 906 medical, pharmaceutical, and dental students. Data were collected through a self-reporting questionnaire.Conclusions The Vietnamese version of the PSQI scale demonstrates acceptable reliability. Furthermore, positive correlations were observed between the total PSQI score and components of the BDI-II. A recommended cut-off point above 5 is proposed for identifying sleep disturbances among health sciences students using the Vietnamese PSQI. Conclusions The study affirms the reliability of the Vietnamese PSQI scale, suggesting its suitability for screening sleep disturbances among health sciences students in Vietnam. PSQI component scores significantly correlated with total BDI-II score.

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        ALKBH8B, a Putative RNA Demethylase, Plays a Role in the Response of Arabidopsis to Salt Stress and Abscisic Acid

        Huong Trinh Thi,Yang Zhengmei,Ngoc Le Nguyen Tieu,강훈승 한국식물학회 2022 Journal of Plant Biology Vol.65 No.4

        RNA methylation and demethylation, which are mediated by RNA methyltransferases (referred to as “writers”) and demethylases (referred to as “erasers”), respectively, have increasingly been demonstrated to play crucial roles in stress responses as well as in plant development. The Arabidopsis thaliana genome encodes thirteen AlkB homolog (ALKBH) proteins that are potential RNA demethylases; however, the functions of most ALKBHs are yet to be determined. In this study, we characterized the function of a potential eraser protein ALKBH8B (At4g02485) during seed germination and seedling growth of Arabidopsis under abiotic stresses. Confocal analysis of the ALKBH8B-GFP expression confirmed that ALKBH8B is localized in the nucleus. The loss-of-function alkbh8b mutant exhibited no apparent phenotypes compared with the wild type under normal and abiotic stress conditions. In contrast, the ALKBH8B-overexpressing transgenic Arabidopsis plants displayed salt-tolerant phenotypes exhibiting better seedling growth and survival rate than wild-type under salt stress. Notably, salt tolerance of the transgenic plants was associated with up- and down-regulation of the expression of several m6A-modified salt stress positive and negative regulators, respectively. Moreover, cotyledon greening and seedling growth of the ALKBH8B-overexpressing transgenic plants were much higher than those of wild type upon abscisic acid (ABA) application, and the transcript levels of ABA signaling-related genes, such as ABI3 and ABI4 were markedly decreased in the transgenic plants. Importantly, global m6A levels decreased in the ALKBH8B-overexpressing transgenic plants as compared with the wild type. Collectively, these results indicate that ALKBH8B plays a role in the response of Arabidopsis to salt stress and ABA via modulating the expression of salt stress- or ABA signaling-related genes.

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