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- 저자 : Neelu Jain (검색결과 3 건)
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An Insight of Nanomaterials in Tissue Engineering from Fabrication to Applications
Sharma Ritika,Kumar Sanjeev,Bhawna,Gupta Akanksha,Dheer Neelu,Jain Pallavi,Singh Prashant,Kumar Vinod 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.5
Tissue engineering is a research domain that deals with the growth of various kinds of tissues with the help of synthetic composites. With the culmination of nanotechnology and bioengineering, tissue engineering has emerged as an exciting domain. Recent literature describes its various applications in biomedical and biological sciences, such as facilitating the growth of tissue and organs, gene delivery, biosensor-based detection, etc. It deals with the development of biomimetics to repair, restore, maintain and amplify or strengthen several biological functions at the level of tissue and organs. Herein, the synthesis of nanocomposites based on polymers, along with their classification as conductive hydrogels and bioscaffolds, is comprehensively discussed. Furthermore, their implementation in numerous tissue engineering and regenerative medicine applications is also described. The limitations of tissue engineering are also discussed here. The present review highlights and summarizes the latest progress in the tissue engineering domain directed at functionalized nanomaterials.
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Lamba Kavita,Kumar Mukesh,Singh Vikram,Chaudhary Lakshmi,Jain Neelu,Saini Anil Kumar,Gupta Vijeta,Singh Akhlash Pratap 한국작물학회 2024 Journal of crop science and biotechnology Vol.27 No.4
Yellow rust, caused by Puccinia striiformis Westend. f. sp. tritici Erikss. (Pst), is one of the most damaging wheat diseases because of their widespread distribution and ability to produce new races that can attack cultivars that were previously resistant. In this study characterization of 48 wheat genotypes was done using 51 primers comprised of stripe rust gene-specific markers and found that WH730 and WH1142 possess the highest number of rust resistant genes while WH1263 bears the lowest Yr genes. These identified resistant Yr genes were exhibited at different frequencies. The resistant genes like, Yr6, Yr18, Yr46, Yr53 and Yr61 were absent in all the 48 studied wheat genotypes. Yr35 and Yr51 had the highest frequency (95.3 and 91.6%, respectively), while Yr16 related to xwmc177 and xwmc18 had lower frequencies (8.0 and 10.4%, respectively). Genetic diversity analysis using 61 molecular markers, and population structure analysis grouped the population into three clusters. Association study of morph-physiological traits showed that the marker xgdm160 was strongly associated with two traits i.e. days to anthesis and days to heading at P value 0.0031 and 0.0048 (P < 0.01), respectively, and showed the highest phenotypic variance (R2). These assessed markers might help develop novel genotypes with numerous genes of resistance against yellow rust races in wheat genotypes by marker-assisted pyramiding.
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Exploration of Heat Stress-Responsive Markers in Understanding Trait Associations in Wheat
Manjunatha P. B.,Nivedita Sinha,Hari Krishna,Divya Chauhan,Pawan Kumar,Ranjeet R. Kumar,Neelu Jain,P. K. Singh,G. P. Singh 한국식물학회 2021 Journal of Plant Biology Vol.64 No.2
Heat stress (HS) is detrimental to wheat production and productivity globally. To combat HS, several genetic, molecular, and genomic approaches have been employed in the past. Analyzing the physiochemical mechanisms and the important regulatory genes involved is the key to develop HS tolerant plants. In the present work, a total of 243 novel simple sequence repeat (SSR) markers developed from stress-associated genes identified through RNA-seq were used for understanding marker–trait associations. 37 SSRs were found to be clearly polymorphic and among these, 28 SSR loci were significantly associated with component traits of HS tolerance. The polymorphic SSRs were validated for diversity analysis on a subset of 85 genotypes. The genotypes were grouped into four clusters representing diverse and similar alleles imparting HS tolerance in Indian and exotic genotypes. Additionally, 28 genes selected for the expression analysis confirmed that 15 genes were induced under HS in the thermotolerant WH1021 and Raj3765 and repressed in thermosusceptible HD2009 cultivar. Hence, the information on traits associated with candidate genes and the SSR markers overlying on the gene will enhance our understanding of thermotolerance mechanism operating in wheat and will help the breeders in the precise development of heat-tolerant genotypes through marker-assisted selection (MAS).
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