http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Navacchi Oriano (검색결과 1 건)
- 무료
- 기관 내 무료
- 유료
-
In vitro shoot regeneration from leaves of Pyrus communis L. rootstock and cultivars
Ricci Angela,Mezzetti Bruno,Navacchi Oriano,Sabbadini Silvia 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.3
The influence of TDZ on adventitious shoot regeneration from leaves of the pear cultivars Conference and Abate Fétel, and the rootstock Farold®87 was investigated. Our main aim was to set up efficient in vitro regeneration protocols for all these pear genotypes by using expanding leaves from elongated shoot cultures as starting plant material. Our best results in terms of percentage of regeneration were achieved by using half-strength Murashige and Skoog basal medium supplemented with 1 μM NAA, combined with 13.5 μM TDZ for Conference (87.3%) and Farold®87 (53.3%), and 9 μM TDZ for Abate Fétel (68%). The impact on leaf organogenesis of the antibiotics timentin, cefotaxime, and carbenicillin, alone or in combination, usually used for the control of Agrobacterium overgrowth, and of kanamycin, commonly used for the selection of putatively transformed plants, were also evaluated to be exploited in future transformation trials. In general, the use of carbenicillin (475 mM), cefotaxime/carbenicillin (210/238 mM) and cefotaxime (630 mM) did not negatively affect the regeneration efficiency of Conference, Abate Fétel and Farold®87, respectively. The use of 4 μM kanamycin should be suitable to select transformed shoots from Abate Fétel and Farold®87 leaves, while a lower concentration or a different selection strategy should be applied for Conference. We report new regeneration and selection protocols usable for the application of new biotechnologies in the genetic improvement of pear cultivars and rootstocks.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
