http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Generation of glycoengineering mice using genome editing technologies
Nan-Ee Lee,Sun Hee Kim,Jeong-Heon Ko,Yong-Sam Kim 한국당과학회 2016 한국당과학회 학술대회 Vol.2016 No.07
Genome editing technologies are versatile tools to delete, insert, or correct a certain span of nucleotide sequences under viable conditions. The generation of model animals became quite easier and has been facilitated due to the development of genome editing methods. We aim to generate glycoengineering mice using CRISPR/Cas9 system that have modified glycan structures either systemically or at a specific proteins. One of the engineering mice is a glycan-humanized mouse that mimics glycan structures of human. Another is the development of aglycosylated antibody-producing mice for precision diagnosis. These glycoengineering mice are expected to provide more efficient preclinical model mice for drug development as well as antibody-producing hosts for personalized, precision medicine.
Kim, Sun Hee,Lee, Nan-Ee,Lee, Jong Seok,Shin, Jeong Hwan,Lee, Ju Yeon,Ko, Jeong-Heon,Chang, Chulhun Ludgerus,Kim, Yong-Sam American Society for Microbiology 2016 Journal of clinical microbiology Vol.54 No.6
<P>Point-of-care (POC) diagnostic testing of tuberculosis (TB) is a tremendous unmet need. In this study, four urinary mycobacterial antigens were identified through two independent approaches using IgG capture and immunodepletion methods. Among these, ModC was validated by a multiple reaction monitoring (MRM) method. As expected, the biomarkers elevated the clinical validity of TB diagnosis when combined with preexisting markers.</P>
Seong Kook Jeon,Jin Suk Park,Jeong Gu Kang,Sun Hee Kim,Nan Ee Lee,Jung Mi Lee,Subin Moon,Dae In Ha,Do Yon Kim,Jeong-Heon Ko,Yong-Sam Kim 한국당과학회 2017 한국당과학회 학술대회 Vol.2017 No.01
Genome editing technology promises to provide versatile tools for the generation of various model cell lines, plants and animals as well as for gene therapy by gene-editing in a target-specific manner. To date, three distinct modes of genome editing technologies have been introduced and extensively investigated in experimental settings, and attempted for use in clinical settings. Despite the revolutionized efficiency and sophistication in gene editing owing to development of CRISPR/Cas9, there remains technical limitations for currently available programmable nucleases in the routine use in experimental and clinical settings. Here, we introduce a universal genome editing technology (UGET) that relies on gene targeting through simple base pairings between a 20-28 nt nucleotide probe and a stretch of single-strand target DNA at the replication fork. UGET has no target limitations, shows lower-than-expected off-target incidence, is straightforward to use, and is compatible to gene correction via HDR. In particular, an overall construct size is less than 2.3kb, which means a high flexibility for use in gene therapy using viral vectors including adenovirus-associated viruses as well as for agrobacterium-based transformation in plants.