한국인 성인 남성 폐쇄성수면무호흡 환자의 측모 두부 방사선계측학적 비교

황상희,박인숙,남기영,김종배,조용원,서영성,안병훈,박신구,박효상 대한치과교정학회 2008 대한치과교정학회지 Vol.38 No.3

본 연구는 비만도에 따른 한국인 남성 폐쇄성수면무호흡 환자의 측모 두부 방사선계측학적 특성을 파악하기 위하여 시행되었다. 이를 위하여 계명대학교 의과대학 동산의료원 수면클리닉에 수면장애를 주소로 내원하여 수면다원검사 후 치과에서 측모 두부방사선계측사진 촬영을 한 87명의 성인 환자들을 체질량지수(BMI)와 수면무호흡지수(AHI)에 따라 비비만 단순코골이군(Non-obese, simple snorers), 비만 단순코골이군(Obese, simple snorers), 비비만 수면 무호흡군(Non-obese, OSA patients), 비만 수면무호흡군(Obese, OSA patients)의 4군으로 나누어 비교하였다. 그결과, 4군 중 비만 수면무호흡군의 수면무호흡지수가 가장 컸으며, 비만 수면무호흡군보다 비비만 수면무호흡군의 하악각이 더 크고 혀 길이는 더 작았다. 또한, 비비만 수면무호흡군보다 비만수면무호흡군의 설골이 더 전하방에 위치하였고, 수면무호흡지수에 영향을 미치는 기여 인자는 비만 수면무호흡군에서는 혀 길이, 비비만 수면무호흡군에서는 설골의 후방위치였다. 이처럼 비만 수면무호흡 환자와 비비만 수면무호흡 환자의 측모 두부방사선계측학적 특성과 기여 인자가 다르게 나타나므로, 치료방법도 따라서 다르게 선택해야 할 것이다. 비만 수면무호흡 환자들에게는 먼저 체중감량이 권고되어야 할 것이고, 비비만 수면무호흡 환자들은 폐쇄부위에 따라 구강 내 장치나 Nasal CPAP (continuous positive airway pressure), UPPP (uvulopalatopharyngoplasty) 등이 추천될 수 있을 것이다. Objective: The purpose of this study was to compare the cephalometric measurements of obese and non-obese Korean male patients with obstructive sleep apnea syndrome (OSA). Methods: Eighty-seven adults who had visited the Sleep Disorder Clinic Center in Keimyung University, Daegu, Korea were examined and evaluated with polysomnography (PSG) and lateral cephalogram. They were divided into 4 groups (non-obese simple snorers, obese simple snorers, non-obese OSA patients, obese OSA patients) according to AHI (Apnea-Hypopnea Index) and BMI (Body Mass Index). Results: The obese OSA group had the highest AHI among the 4 groups. The non-obese OSA group had a significantly steeper mandibular angle and shorter tongue length than the obese OSA group. The hyoid bone of the obese OSA group was positioned anterior and inferior as compared with the non-obese OSA group. Multiple regression analysis showed that tongue length in the obese OSA group and retroposition of hyoid bone in the non-obese OSA group were significant determinants for the severity of AHI. Conclusions: From a cephalometric point of view, the obese and non-obese pateints with OSA may be characterized by different pathogeneses. Therefore, they have to be managed by individualized treatment. For the obese OSA patients, weight control must be advised as a first choice and for the non-obese OSA patients, oral appliance, nasal CPAP, UPPP and others could be chosen according to the obstructive sites.

삼풍백화점 붕괴사고 희생자들의 신원확인을 위한 유전자검사

남용석,이혜린,김경훈,김희선,이희석,황적준 大韓法醫學會 1996 대한법의학회지 Vol.20 No.1

A DNA typing was performed to identify decomposed body remains from Sampoong Department mass disaster in June 1995. These body parts include bone fragment, skin tissue, hairs, from which the extracted DNAs were highly degraded. Two VNTR loci, 4STR loci, and amelogenin gene were chosen for AMP-FLP, and mtDNA sequence analysis for the confirmation of maternal relationship. The results of AMP-FLP of the selected polymorphic loci showed different sucess rate for PCR. DIS80 and D17S5 loci were amplified successfully form 64.5%, and 67% of the samples, respectively. HUMTHOI, HUMCSF1PO, and HUMTPOX loci were amplified successfully from 90.3% of the samples each. HUMACTBP2 and amelogenin was amplified in 87% of the cases submitted. THE DNA types of 33 remains were compared with those of 81 bereaved families consisting of 173 member. Thirty three samples were reduced to 28 in numbers according to results of the same DNA types. Among them, the DNA types of 15 remains matched with those of bereaved families and the identified remains were reconfirmed by amelogenin sex typing and mitochondrial DNA sequence analysis. The others were not identified a family by failures of PCR amplification or non-matching of DNA types. Also it is confirmed that one hair sample should be artificial by non-digestion of protease and another be animal bone by result of dot blotting with human Alu probe. Our results indicate that multiplex PCR system consisting of several STR loci like HUMCSF1PO, HUMTPOX, and HUMTHO1 is more effective for the identification of highly decomposed human remains from mass disaster.

螢光像 測定法으로 分析한 HUMACTBP2 遺傳座位에 대한 韓國人의 遺傳的 多樣性과 法醫學的 有用性

황적준,남용석,최하주,이혜린,한길로 大韓法醫學會 1997 대한법의학회지 Vol.21 No.1

The HUMACTBP2 locus was investigated to collect population genetic data in the Korean population and to evaluate the applicability for the forensic field. An Automatic fluorescent-based sequencer (377 automatic DNA sequencer, ABI) was used to detect amplified fragments of the HUMACTBP2 locus electrophoresed on 4% denaturing polyacrylamide sequencing gels. ACTBP2 allelic ladder consisting of different sizes of 18 alleles was constructed and employed as an internal size standard in combination with a GS-350 size standard for precision of allele-band sizing. By utilizing different fluorescent dyes, both the allelic ladders and samples were able to be analyzed in the same lane by 99% precision of allele-band sizing. Among the Korean population (n=224), 26 alleles in the range of 239-313 bp are determined. Allele No. 6 is found 45 times (0.100) which is mostly frequent, and the rest of allele is distributed with their relative frequency of 0.112-0.100. The comparison between observed and expected numbers of homozygous and heterozygous individuals confirms that ACTBP2 locus is in the state of Hardy-Weinberg equilibrium among the Korean population. The heterozygosity is 0.9389±0.0034(93.89%), and the power of discrimination)PD) and power of exclusion(PEX) are calculated to be 0.991(99.1%) and 0.890(89.0%), respectively, showing the high informativeness for individual identification. Thus, these results mean that the HUMACTBP2 locus can effectively be used for the forensic application.

韓國人의 HLA DQA1 遺傳座位에 대한 集團 遺傳學的 特性

남용석,김희선,이희석,이혜린,황적준 大韓法醫學會 1995 대한법의학회지 Vol.19 No.1

Using reverse dot blotting technique, genotype of HLA DQAl locus have been determined from 142 unrelated Korean individuals. Twenty genotypes were found from possible twenty one genotypes - the missing one was A2/A2 that had lowest expected frequency. All of known 6 alleles were found with each of its frequency being 15.1% for A1. 1, 16.6%for A1.2, 12.7% for A1.3, 11.6% for A2, 25.7% for A3 and 18.3% for A4. After X?-test(p>0.1), G-test(p>0.05), and by comparision of expected (0.82) and observed heterozygosity(0.81), the population was confirmed to be on the state of Hardy-Weinberg equilibrium. The gene diversity(0.82) of Korean population, which generally thought to be a group of single unity, actually was higher than that of most other populations. The pattern of alelic distribution was different from that of other populations, especially allele A1.3 which displayed heterogeneity between other goups with significance(p<0.01), as it turned out to have anthropological significance. After all, this HLA DQA1 system, even though its small number of alleles, having high degree of heterozygosity, was proven to be effective in individual identification, and paternity testing in Koean population.

pV47-2 다좌위탐식자를 이용한 인체 게놈에서 다형성 유전좌위의 분리

남용석,이혜린,한길로,황적준 大韓法醫學會 1997 대한법의학회지 Vol.21 No.2

Two polymorphic loci, so- called FS106 and FS185, have been isolated from the human genome, using a multilocus probe pV47-2, which is extensively used in Korea for forensic investigation such as resolving paternity disputes. Among the several plaques selected from λ Fix-II genomic libraries, fourteen clones have been characterized. Restriction maps of 14 clones were constructed to define the flanking as well as repeat parts. The repeat-free flanking DNA fragments were tested for single locus specific polymorphism, and repeat containing DNA fragments were sequenced for the design of PCR primers. None of the repeat-free flanking DNA fragments was not shown any polymorphisms by RFLP analysis. The (GGT)??-rich sequences in most of repeat containing DNA fragments were identified by sequencing analysis. Most of repetitive sequences consists of major units of (GGT)??, but a regular repetition pattern can not be found in all clones. Two sets of primers designed from flanking sequences of repeat containing DNA fragments were shown length polymorphisms by PCR analysis, when tested in 50 unrelated individuals. Three and four alleles were detected at FS106 and 0.58 for FS185. In addition, two loci, FS106 and FS185, have been mapped on chromosome 5 and 3, respectively, by somatic cell hybrid analysis.

創傷의 經過時間

黃迪駿,李羲碩,南容碩 大韓法醫學會 1994 대한법의학회지 Vol.18 No.2

When examining a victim of viloence, no forensic pathologist is fulfilling his role when one confines the report to merely a numbered lists of wounds found on the corpse. Of course, the nature, exact position, and dimensios of every injury should be described, and it should e photographed. However, the distinction between antemortem and postmortem injuries and their proper timing is one of the cardinal problems of froensic medicine. It help not only to convict guilty but also to acquit persons who are suspect but in factnot guilty. The cause of death is sometimes of less importance than the recsotruction of events, which may become possible with careful examination of the wounds and their proper timing. As with time of death, it can be a very dificult matter in forensic medical investigations to determine whether a wound found at autopsy was inflicted before or after death, and if ante-mortem, how long before death was it sustained? Unfortunately, as with so many problems, biological variability introduces a wide range of uncertainty about the time of wound, so that a range of probabilities can be offered, but never a definite time interval. Therefore, the purpose of this paper is to discuss pros and cons about various dating methods of ante-mortem wound that have been published.

삼풍백화점 붕괴사고의 신원확인을 위한 다형성 유전좌위의 선정

황적준,남용석,이희석,이혜린,김희선,김경훈 內務部 國立科學搜査硏究所 1996 國立 科學 搜査 硏究所 年報 Vol.28 No.-

토끼에서 Etofenprox와 Spinosad 합제의 국소자극성 시험

황윤환,김명석,송인배,박병권,양승호,임용현,송치용,안성한,남현수,임정교,정상일,명노일,윤효인 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2008 動物醫科學硏究誌 Vol.15 No.1

This study was performed to evaluation the skin and eye irritation of etofenprox 8% and spinosad 4% combination in male New Zealand White rabbits. In skin irritation test, the combination of etofenprox and spinosad did not showed any change of body weight but caused slight skin irritation with the P.I.I. (primary irritation index) value 0.25. The etofenprox and spinosad combination showed severe eye irritation 1 h, 24 h, 48 h, and 72 h after ocular treatment, having the A.O.I. (acute ocular irritation) value of 37.3. Therefore, these result indicated that the combination of etofenprox and spinosad might be irritant formulation on eye and skin.

Ventriculosubgaleal shunt의 임상적 경험

남궁원철,박상근,김태홍,신형식,황용순,김상진 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.2

Object : Ventriculosubgaleal(VSG) shunt is considered to be useful and safe ventricluar diversion method for the patient for whom ventriculoperitoneal(VP) shunt cannot be carried out because of significant particulate debirs within the CSF, recent surgeries, or infections that were considered substantial enough to impair shunt function or to increase the risk of infection. To clarify the efficacy of VSG shunt for temporary ventricular decompression we analyzed our clinical trial cases. Patients and Methods : VSG shunt was carried out in 8 patient with acute hydrocephalus between February 2000 and June 2000. The causes of acute hydrocephalus were as follows: spontaneous intracerebral hemorrahage and intraventricular hemorrhage in four patients, spontaheous subarachnoid hemorrahge in three patients, tuberculous meningitis in one patient. With these 8 cases we compared pre-operative and post-operative clinical conditions and ventricular size in the brain CT scan. Results : Even though the ventricular size was not changed statistically there was significant clinical improvement between preoperative Glasgow coma scale(GCS)(7.25) and postoperative GCS(9.25). Six patients died as a result of causes unrelated to the VSG shunt. There were no VSG shunt infection. A permanent ventriculoperitoneal shunt surgery was needed in two patients. Conclusion : The VSG shunt is a simple and safe method of treatment for acute hydrocephalus instead of extraventricular drainage in the patients who cannot be underwent a permanent shunt immediately due to some reasons. Even though VSG shunt were free from infection, our preliminary clinical trials suggests that its efficacy seems to be doubtful. Further studies with lots of cases would be needed for its clinical application.

상관기 진폭 보상에 따른 판별력 조사

황수용,이상이,김남선 서울産業大學校 1996 논문집 Vol.44 No.1

광 JTC는 실시간 상관 시스템에 응용이 가능하다는 장점에 반하여 위상 혹은 진폭성분만을 별도로 처리하기는 어려운 상태에 있었으나, 최근 POC와 POF에 유사한 상관특성을 나타낼수 있는 광 BPEJTC, HPEJTC등이 제안되면서 위상과 진폭이 별도로 처리될 수 있게 되었다. 그러나, 이러한 시스템들은 위상과 진폭이 상관성능에 어떠한 영향을 미치는재 평가하기는 어려운 상태에 있었다. 따라서, 본 논문에서는 진폭이나 위상 성분의 비중을 변동시키며 상관성능을 분석 할 수 있는 것으로 ACMF를 기초로 하는 JTC 시스템인 ACJTC를 수학적 해석을 통해 비교분석하였다. ACJTC는 ACMF의 특성상 위상과 진폭 성분의 비중을 가변시킬수 있으므로 잡음 및 유사표적에 대하여 우수한 상관 판벽력을 나타내게 된다. 이러한 특성을 조사하기 위해 자연게 배경에 다중 표적 영상을 주기적으로 배치하고 컴퓨터 시뮬레이션하였으며, 광 JTC를 통한 실시간 상관 시스템의 구현 가능성을 제시하였다. The optical JTC system has the advantage of being applied to the real-time correlation system, but it has some difficulties that is amplitude and phase signals cannot be separately processed. Recently to solve those problems the BPEJTC and HPEJTC systems having the same characteristics of that of the POC and POF have been proposed. But there is no quantitive analysis how much the amplitude and phase signals are contributed to the correlation outputs respectively. In this paper, a ACJTC system based on the conventional ACMF is discussed through the mathematical analysis. The good correlation discrimination of the system is also analyzed by changing the relative contribution of the phase and amplitude signals for some similar targets. From the successful computer simulation results on the scenario where three same targets are periodically located on the nature background, the possibility of the high performance real-time correlation system based on the optical ACJTC is suggested.