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- 검색키워드
- 저자 : Na Rae Han (검색결과 134 건)
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Han, Na Rae,Baek, Song,Lee, Yongjin,Lee, Joohyeong,Yun, Jung Im,Lee, Eunsong,Lee, Seung Tae The Korean Society of Animal Reproduction and Biot 2020 한국동물생명공학회지 Vol.35 No.1
The establishment of porcine embryonic stem cells (ESCs) from porcine somatic cell nuclear transfer (SCNT) blastocysts is influenced by in vitro culture day of porcine reconstructed embryo and feeder cell type. Therefore, the objective of the present study was to determine the optimal in vitro culture period for reconstructed porcine SCNT embryos and mouse embryonic fibroblast (MEF) feeder cell type for enhancing colony formation efficiency from the inner cell mass (ICM) of porcine SCNT blastocysts and their outgrowth. As the results, porcine SCNT blastocysts produced through in vitro culture of the reconstructed embryos for 8 days showed significantly increased efficiency in the formation of colonies, compared to those for 7 days. Moreover, MEF feeder cells derived from outbred ICR mice showed numerically the highest efficiency of colony formation in blastocysts produced through in vitro culture of porcine SCNT embryos for 8 days and porcine ESCs with typical ESC morphology were maintained more successfully over Passage 2 on outbred ICR mice-derived MEF feeder cells than on MEF feeder cells derived from inbred C57BL/6 and hybrid B6CBAF1 mice. Overall, the harmonization of porcine SCNT blastocysts produced through in vitro culture of the reconstructed embryos for 8 days and MEF feeder cells derived from outbred ICR mice will greatly contribute to the successful establishment of ESCs derived from porcine SCNT blastocysts.
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Na Rae Han,Song Baek,Yongjin Lee,Joohyeong Lee,Jung Im Yun,Eunsong Lee,Seung Tae Lee 한국동물생명공학회(구 한국동물번식학회) 2020 Journal of Animal Reproduction and Biotechnology Vol.35 No.1
The establishment of porcine embryonic stem cells (ESCs) from porcine somatic cell nuclear transfer (SCNT) blastocysts is influenced by in vitro culture day of porcine reconstructed embryo and feeder cell type. Therefore, the objective of the present study was to determine the optimal in vitro culture period for reconstructed porcine SCNT embryos and mouse embryonic fibroblast (MEF) feeder cell type for enhancing colony formation efficiency from the inner cell mass (ICM) of porcine SCNT blastocysts and their outgrowth. As the results, porcine SCNT blastocysts produced through in vitro culture of the reconstructed embryos for 8 days showed significantly increased efficiency in the formation of colonies, compared to those for 7 days. Moreover, MEF feeder cells derived from outbred ICR mice showed numerically the highest efficiency of colony formation in blastocysts produced through in vitro culture of porcine SCNT embryos for 8 days and porcine ESCs with typical ESC morphology were maintained more successfully over Passage 2 on outbred ICR mice-derived MEF feeder cells than on MEF feeder cells derived from inbred C57BL/6 and hybrid B6CBAF1 mice. Overall, the harmonization of porcine SCNT blastocysts produced through in vitro culture of the reconstructed embryos for 8 days and MEF feeder cells derived from outbred ICR mice will greatly contribute to the successful establishment of ESCs derived from porcine SCNT blastocysts.
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Identification of a Technique Sorting Effectively Spermatogonial Stem Cells from Mouse Testes
Na Rae Han,Hye Jin Park,Hyun Lee,Seung Tae Lee 한국동물생명공학회(구 한국동물번식학회) 2018 발생공학 국제심포지엄 및 학술대회 Vol.2018 No.06
Spermatogonial stem cells (SSCs) can produce sperms transferring genetic information into the next generation in seminiferous tubule of testes. Accordingly, an efficient isolation technique of SSCs with extremely low numbers from testes is required for successful downstream researches related to maintenance, differentiation and cryopreservation of SSCs. To date, a variety of isolation techniques in a variety of species have been used for retrieving SSCs from testicular tissue. However, comparison of their efficiency has been not revealed clearly. Accordingly, among isolation methods described previously, we tried to elucidate a technique showing the best isolation efficiency in the retrieval of SSCs from testes derived from mice. For these, SSCs were isolated from mouse testis as follows: differential plating (DP), EpCAM, Thy1 or GFRα1 antibody-based magnetic-activating cell sorting (MACS) post-DP, EpCAM, Thy1 or GFR α1 antibody-based MACS, EpCAM, Thy1 or GFRα1 antibody-based MACS post-MACS based on GFRα1 antibody (double MACS for positive selection), and CD34 or α-SMA antibody-based MACS post-MACS based on GFRα1 antibody (double MACS for negative selection). Subsequently, SSCs isolated from each method were stained by alkaline phosphatase (AP) staining and percentage of AP positive SSCs was compared to find an optimal method among isolation method candidates. As the results, SSCs isolated by DP for 8 h showed numerically the highest percentage of AP positive SSCs. In case of MACS post-DP, SSCs isolated by MACS based on Thy1 antibody post-DP (MACSThy1 post-DP) revealed numerically higher percentage of AP positive cells than those on EpCAM and GFRα1 antibodies post-DP. Moreover, numerically the highest percentage of AP positive SSCs was detected when SSCs were isolated from mouse testis by MACS based on GFRα1 (MACSGFRα1) compared to EpCAM and Thy1 antibodies. In case of double MACS for positive selection, the usage of EpCAM antibody in the second MACS post-GFRα1 antibody-based MACS (double MACSGFRα1/EpCAM) showed numerically the highest percentage of AP positive SSCs compared to Thy1 and GFRα1 antibodies. On the other hands, after GFRα1 antibody-based MACS sorting, the usage of α-SMA antibody in the second MACS (double MACSGFRα1/α-SMA) for negative selection showed numerically higher percentage of AP positive SSCs than CD34 antibody. The subsequent comparison of isolation efficiency derived from each method demonstrated that MACSGFRα1 and double MACSGFRα1/EpCAM resulted in significantly the best isolation efficiency. Accordingly, we could elucidate that MACSGFRα1 and double MACSGFRα1/EpCAM were techniques sorting effectively SSCs from mouse testes.
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Na Rae Han,Song Baek,Yongjin Lee,Joohyeong Lee,Jung Im Yun,Eunsong Lee,Seung Tae Lee 한국동물생명공학회(구 한국동물번식학회) 2019 Journal of Animal Reproduction and Biotechnology Vol.34 No.2
Although somatic cell nuclear transfer (SCNT)-derived embryonic stem cells (ESCs) in pigs have great potential, their use is limited because the establishment efficiency of ESCs is extremely low. Accordingly, we tried to develop in-vitro culture system stimulating production of SCNT blastocysts with high performance in the colony formation and formation of colonies derived from SCNT blastocysts for enhancing production efficiency of porcine ESCs. For these, SCNT blastocysts produced in various types of embryo culture medium were cultured in different ESC culture medium and optimal culture medium was determined by comparing colony formation efficiency. As the results, ICM of porcine SCNT blastocysts produced through sequential culture of porcine SCNT embryos in the modified porcine zygote medium (PZM)-5 and the PZM-5F showed the best formation efficiency of colonies in α-MEM-based medium. In conclusion, appropriate combination of the embryo culture medium and ESC culture medium will greatly contribute to successful establishment of ESCs derived from SCNT embryos.
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Protective effect of porcine placenta in a menopausal ovariectomized mouse
Han, Na-Ra,Park, Chan-Lee,Kim, Na-Rae,Kim, Hee-Yun,Yoou, Myoung-Schook,Nam, Sun-Young,Moon, Phil-Dong,Jeong, Hyun-Ja,Kim, Hyung-Min BioScientifica 2015 Reproduction Vol.150 No.3
<P>Menopause is a significant physiological phase that occurs as women's ovaries stop producing ovum and the production of estrogen declines. Human placenta and some amino acids are known to improve menopausal symptoms. In this study, we investigated that porcine placenta extract (PPE) and arginine (Arg), a main amino acid of PPE, would have estrogenic activities in ovariectomized (OVX) mice as a menopause mouse model, human breast cancer cell line (MCF-7) cells, and human osteoblast cell line (MG-63) cells. PPE or Arg significantly inhibited the body weight and increased the vagina weight compared to the OVX mice. PPE or Arg ameliorated the vaginal atrophy in the OVX mice. The levels of 17β-estradiol and the activities of alkaline phosphatase (ALP) were significantly increased by PPE or Arg in the serum of OVX mice. Trabecular bone parameters such as bone mineral density and porosity were also improved by PPE or Arg in the OVX mice. In the MCF-7 and MG-63 cells, PPE or Arg significantly increased the cell proliferation, estrogen receptor β mRNA expression, and estrogen-response elements luciferase activity. Finally, PPE or Arg increased the activations of ALP and extracellular signal-regulated kinase 1/2 in the MG-63 cells. These results indicate that PPE or Arg would have estrogenic and osteoblastic activity. Therefore, PPE or Arg may be useful as new pharmacological tools for treating menopausal symptoms including osteoporosis.</P><P><B>Free Korean abstract</B>: A Korean translation of this abstract is freely available at http://www.reproduction-online.org/content/150/3/173/suppl/DC1.</P>
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Han, Na-Ra,Moon, Phil-Dong,Kim, Na-Rae,Kim, Hee-Yun,Jeong, Hyun-Ja,Kim, Hyung-Min Institute for Advanced Research in Asian Science a 2017 The American journal of Chinese medicine Vol. No.
<P><I>Schisandra chinensis</I> (SC) and its main constituent, schizandrin (SCH) exhibit anti-inflammatory and anti-allergic activities. Allergic and inflammatory reactions are aggravated via caspase-1 signaling pathway. However, the regulatory effects of SC and SCH on caspase-1 activation have not been clarified yet. In this study, we aimed to clarify the anti-allergic effects of SC and SCH using an ovalbumin (OVA)-sensitized mice and anti-CD3 and anti-CD28 antibodies-stimulated splenocytes. SC or SCH significantly inhibited the levels of immunoglobulin (Ig)E, IgG1, or interleukin (IL)-4 in serum of OVA-sensitized mice. SC or SCH significantly inhibited the levels of IL-6, tumor necrosis factor (TNF)-<TEX>$ \alpha $</TEX>, and IL-1<TEX>$ \beta $</TEX> in spleen of the OVA-sensitized mice. SC or SCH significantly suppressed the expression of caspase-1 and receptor-interacting protein (RIP)-2 in spleen of the OVA-sensitized mice. In activated splenocytes, SC or SCH significantly decreased the expression of caspase-1 and RIP-2 as well as the production of IL-6 and TNF-<TEX>$ \alpha $</TEX>. We suggest that SC and SCH exert an anti-allergic effect by down-regulating caspase-1 signaling.</P>
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Na Rae Han,Hyun Lee,Jung Im Yun,Choonghyo Kim,Jae Yeon Hwang,Kyu Hyun Park,Seung Tae Lee 한국수정란이식학회 2017 한국동물생명공학회지 Vol.32 No.2
Mesenchymal stem cells (MSCs) have been considered an alternative source of neuronal lineage cells, which are difficult to isolate from brain and expand in vitro. Previous studies have reported that MSCs expressing Nestin (Nestin+ MSCs), a neuronal stem/progenitor cell marker, exhibit increased transcriptional levels of neural development-related genes, indicating that Nestin+ MSCs may exert potential with neurogenic differentiation. Accordingly, we investigated the effects of the presence of Nestin+ MSCs in bone-marrow-derived primary cells (BMPCs) on enhanced neurogenic differentiation of BMPCs by identifying the presence of Nestin+ MSCs in uncultured and cultured BMPCs. The percentage of Nestin+ MSCs in BMPCs was measured per passage by double staining with Nestin and CD90, an MSC marker. The efficiency of neurogenic differentiation was compared among passages, revealing the highest and lowest yields of Nestin+ MSCs. The presence of Nestin+ MSCs was identified in BMPCs before in vitro culture, and the highest and lowest percentages of Nestin+ MSCs in BMPCs was observed at the third (P3) and fifth passages (P5). Moreover, significantly the higher efficiency of differentiation into neurons, oligodendrocyte precursor cells and astrocytes was detected in BMPCs at P3, compared with P5. In conclusion, these results demonstrate that neurogenic differentiation can be enhanced by increasing the proportion of Nestin+ MSCs in cultured BMPCs.
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Han, Mi Na,Byeon, Hyeon Seop,Lee, Cho Yeon,Jo, Nam Sin,Lee, Jong Hwa,Jang, Rae Hoon,Kim, Chang Seop,Na, Ki Jeong The Korean Society of Veterinary Service 2019 韓國家畜衛生學會誌 Vol.42 No.4
Newcastle disease (ND) is an infectious poultry disease that caused high mortality and reduced egg production. NDVs are regularly present in the domestic duck population. And ducks play a possible role in the maintenance and transmission of NDVs. While we were monitoring the Avian Influenza, NDVs were isolated from field samples by accident. So we analysed the biological and genetic characteristics of these viruses. Lentogenic NDVs were isolated from two farms among twenty breeder duck farms. The ages of ducks were 39 weeks old in the 'A' farm and 3~72 weeks old in the 'B' farm. And they were not inoculated with the NDVs vaccine. In the biological characteristics, the both viruses which separated from the farm 'A' and 'B' were thermostable. The amino acid sequence of a site from 112 to 119 in the fusion (F) protein was 'GKQGRLIG' which has monobasic motif in the samples of both farms. And this means the separated NDVs are lentogenic. Phylogenetic analysis was performed by entire nucleotide sequence of F protein. The virus strains from the A farm (MN095239) and the B farm (MN095240) belonged to class II genotype I. Using the analysis of whole F protein nucleic acid sequence, the MN095239 (GenBank) had homology with Ulster strain about 99.95% and the MN095239 (GenBank) had homology with KR/CK/KU_LBM255/09 strain about 99.89%. NDV surveillance is needed to investigate epidemiological relationship of domestic breeder duck isolates in Korea.
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Han, Na Rae,Cho, Jae Whan Elsevier 2018 Composites Applied science and manufacturing Vol.109 No.-
<P><B>Abstract</B></P> <P>Covalently bonded hybrids (GO-click-TWNTs) of graphene oxide (GO) and thin-walled carbon nanotubes (TWNTs) with different compositions were synthesized by a click chemistry reaction between alkyne-moiety GO and azide-moiety TWNTs. Polyurethane (PU) nanocomposites with the GO-click-TWNT hybrids were prepared to investigate an effect of the GO-click-TWNT hybrids on the mechanical properties of the PU nanocomposites. Synthesis of click coupled hybrids was confirmed by Fourier transform infrared, Raman, and X-ray photoelectron spectroscopies. The transmission electron microscopic measurements showed a well-networked structure between the GO and TWNTs in the hybrids. The GO and TWNTs in the hybrids had a synergistic effect on the mechanical properties of the PU nanocomposites. The PU nanocomposites with GO-click-TWNT hybrids with 3:7 GO/TWNT weight composition showed the highest breaking stress and modulus. The synergistic effect of the GO-click-TWNT hybrids was also shown in their electrical conductivity and near-infrared laser-induced photothermal properties.</P>
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