Requirement of β subunit for the reduced voltage-gated Na+ current of a Brugada syndrome patient having novel double missense mutation (p.A385T/R504T) of SCN5A

Na Kyeong Park,Seong Woo Choi,Soon-Jung Park,JooHan Woo,Hyun Jong Kim,Woo Kyung Kim,Sung-Hwan Moon,Hun-Jun Park,Sung Joon Kim 대한생리학회-대한약리학회 2024 The Korean Journal of Physiology & Pharmacology Vol.28 No.4

Mutations within the SCN5A gene, which encodes the α-subunit 5 (NaV1.5) of the voltage-gated Na+ channel, have been linked to three distinct cardiac arrhythmia disorders: long QT syndrome type 3, Brugada syndrome (BrS), and cardiac conduction disorder. In this study, we have identified novel missense mutations (p.A385T/R504T) within SCN5A in a patient exhibiting overlap arrhythmia phenotypes. This study aims to elucidate the functional consequences of SCN5A mutants (p.A385T/R504T) to understand the clinical phenotypes. Whole-cell patch-clamp technique was used to analyze the NaV1.5 current (INa) in HEK293 cells transfected with the wild-type and mutant SCN5A with or without SCN1B co-expression. The amplitude of INa was not altered in mutant SCN5A (p.A385T/R504T) alone. Furthermore, a rightward shift of the voltage-dependent inactivation and faster recovery from inactivation was observed, suggesting a gain-of-function state. Intriguingly, the coexpression of SCN1B with p.A385T/R504T revealed significant reduction of INa and slower recovery from inactivation, consistent with the loss-of-function in Na+ channels. The SCN1B dependent reduction of INa was also observed in a single mutation p.R504T, but p.A385T co-expressed with SCN1B showed no reduction. In contrast, the slower recovery from inactivation with SCN1B was observed in A385T while not in R504T. The expression of SCN1B is indispensable for the electrophysiological phenotype of BrS with the novel double mutations; p.A385T and p.R504T contributed to the slower recovery from inactivation and reduced current density of NaV1.5, respectively.

Expression of the Na⁺-HCO₃⁻cotransporter and its role in pH_iregulation in guinea pig salivary glands

Li, Jingchao,Koo, Na-Youn,Cho, Ik-Hyun,Kwon, Tae-Hwan,Choi, Se-Young,Lee, Sung J.,Oh, Seog B.,Kim, Joong-Soo,Park, Kyungpyo American Physiological Society 2006 American journal of physiology, Gastrointestinal a Vol.291 No.6

<P>Patterns of salivary HCO3<SUP>−</SUP>secretion vary and depend on species and gland types. However, the identities of the transporters involved in HCO3<SUP>−</SUP>transport and the underlying mechanism of intracellular pH (pHi) regulation in salivary glands still remain unclear. In this study, we examined the expression of the Na<SUP>+</SUP>-HCO3<SUP>−</SUP>cotransporter (NBC) and its role in pHiregulation in guinea pig salivary glands, which can serve as an experimental model to study HCO3<SUP>−</SUP>transport in human salivary glands. RT-PCR, immunohistochemistry, and pHimeasurements from BCECF-AM-loaded cells were performed. The amiloride-sensitive Na<SUP>+</SUP>/H<SUP>+</SUP>exchanger (NHE) played a putative role in pHiregulation in salivary acinar cells and also appeared to be involved in regulation in salivary ducts. In addition to NHE, NBC also played a role in pHiregulation in both acini and ducts. In the parotid gland, NBC1 was functionally expressed in the basolateral membrane (BLM) of acinar cells and the luminal membrane (LM) of ducts. In the submandibular gland, NBC1 was expressed only in the BLM of ducts. NBC1 expressed in these two types of salivary glands takes up HCO3<SUP>−</SUP>and is involved in pHiregulation. Although NBC3 immunoreactivity was also detected in submandibular gland acinar cells and in the ducts of both glands, it is unlikely that NBC3 plays any role in pHiregulation. We conclude that NBC1 is functionally expressed and plays a role in pHiregulation in guinea pig salivary glands but that its localization and role are different depending on the type of salivary glands.</P>

Enhancement of the hydrogen productivity in microbial water gas shift reaction by <i>Thermococcus onnurineus</i> NA1 using a pressurized bioreactor

Kim, Min-Sik,Fitriana, Hana Nur,Kim, Tae Wan,Kang, Sung Gyun,Jeon, Sang Goo,Chung, Soo Hyun,Park, Gwon Woo,Na, Jeong-Geol Elsevier 2017 INTERNATIONAL JOURNAL OF HYDROGEN ENERGY - Vol.42 No.45

<P><B>Abstract</B></P> <P>Here, we developed a pressurized bioreactor system that increase carbon monoxide (CO) transfer efficiency in order to enhance the hydrogen productivity in the microbial water gas shift reaction by <I>Thermococcus onnurineus</I> NA1. The effects of CO pressure on the hydrogen production rate, CO consumption rate and the cell growth were investigated using small scale stainless steel bottles at various CO partial pressures. It was found that CO solubility increased by applying pressure can affect hydrogen production positively as long as the increased toxicity of CO is endurable to cells. The hydrogen productivity increased to some extent with CO pressure, but decreased drastically at the pressure higher than 4 bar. On the other hand, the effect of pressure itself on the cell's activity was not as significant as that of CO solubility increase. In the experiments at various system pressures with identical CO partial pressure of 1 bar, more than 80% of the cell activity remains even at total pressure of 10 bar. Also, it was important to determine the appropriate time to increase pressure for preventing excess CO in the reactor. Based on these results, a fermentation strategy for the pressurized system was designed and applied to a 5 L bioreactor with the continuous supply of the gas containing 60% CO. When the pressure was introduced to the bioreactor up to 4 bar at CO limitation condition, the unprecedented high productivity (360 mmol L<SUP>−1</SUP> h<SUP>−1</SUP>) could be obtained.</P> <P><B>Highlights</B></P> <P> <UL> <LI> CO gas solubility increased by pressurizing can be beneficial to H<SUB>2</SUB> production. </LI> <LI> The impact of pressure per se was marginal. </LI> <LI> CO toxicity impacts were reduced by applying pressure at mass transfer limitation condition. </LI> <LI> When the pressure was introduced to the bioreactor, the unprecedented high productivity could be obtained. </LI> </UL> </P>

植物群落 構造의 分析方法에 관한 硏究

朴秉奎,金奈賢 梨花女子大學校 韓國生活科學硏究院 1979 韓國生活科學硏究院 論叢 Vol.23 No.-

경기도 광능의 식물군락의 구조를 Agnew의 식물종간의 유사도 측정방법, Williams and lambert의 계급분류, Bray and Curtis의 ordination 방법 등을 사용하여 분석하였으며, 아물러 분석된 식생의 동형 Group과 토양의 제반 성질 (수분향향, pH, 유기물, 치환성 양이온, 유효인상, 질소)을 결부하여 설명하고자 시도하였으며, 다음과 같은 결과를 얻을 수 있었다. 1. Agnew의 식물종간의 유사도 측정방법으로 출현종을 연관성있는 종들고 구성된 3개의 동형 Group으로 나눌 수 있었다. 즉, GroupⅠ은 가락지나물, 고만이, 물봉선화, 미꾸리낚시, 자랭이, 졸방제비꽃. GroupⅡ는 마, 복분자딸기, 사위질빵, 으름덩굴, GroupⅢ는 남산제비꽃, 노랑제비꽃, 참꽃말이 등으로 이루어져있다. 또한, 위와 같은 3개의 동형 group을 토양의 제반 성질과 결부하여 설명할 수는 없었다. 2. Williams and Lambert의 계급분류 방법으로 전 방형구를 2개의 group으로 나눌 수 있으며, groupⅠ은 미꾸리낚시는 출현하나 마는 출현하지 않는 방형구이며, GroupⅡ는 미꾸리낚시가 출현하지 않는 방형구들이다. 또한 위의 2개의 group을 토양의 제반 성질과 결부하여 설명할 수는 없었다. 3. Bray ans Curtis의 ordination 방법으로 25 Relev'를 좌표상에 도시하였으며, 토양의 제반 성질 중 유기물, 총 질소, 유효인산의 양과 결부하여 설명할 수 있었다. 4. 본 연구에서는 위와 같은 결과에 의하여 식물군락의 구조를 분석하는데 Bray and Curtis의 ordination 방법이 가장 바람직하다고 할 수 있다. A plant Community in Kwangneung was investigated by the orthodox Braun-Blanquet recording technique, subquadrat method, and point quardrat method and analyzed by the method of Agnew's interspecific association, Bray & curtis'polar ordinition, Williams & lambert's normal association analysis. Also the characteristics of soil in study areas (water content, pH, orgnaic matters, exchangeable action = K, Ca, Na, available phosphorus, total nitrogen) were analyzed. As a result of analyses, the plant community invetigated was divided into several groups. At the same time the measurement of the interrelationship between these groups and soil characteristics was attempted and there were correlations by the method Bray & Curtis, but no correlations by the method of Angew and Williams & lambert. Therefore the bray & Curtis' polar ordination method was the best in this study.

Extended Culture of Bone Marrow with Granulocyte Macrophage-Colony Stimulating Factor Generates Immunosuppressive Cells

Na, Hye Young,Sohn, Moah,Ryu, Seul Hye,Choi, Wanho,In, Hyunju,Shin, Hyun Soo,Park, Chae Gyu 한국조명·전기설비학회 2018 한국조명·전기설비학회 학술대회논문집 Vol. No.

<P>Bone marrow-derived dendritic cells (BM-DCs) are generated from bone marrow (BM) cells cultured with granulocyte macrophage-colony stimulating factor (GM-CSF) for a week. In this study we investigated the effect of duration on the BM culture with GM-CSF. Within several months, the cells in the BM culture gradually expressed homogeneous levels of CD11c and major histocompatibility complex II on surface, and they became unable to stimulate allogeneic naïve T cells in mixed lymphocyte reaction (MLR). In addition, when the BM culture were sustained for 32 wk or longer, the BM cells acquired ability to suppress the proliferation of allogeneic T cells in MLR as well as the response of ovalbumin-specific OT-I transgenic T cells in antigen-dependent manner. We found that, except for programmed death-ligand 1, most cell surface molecules were expressed lower in the BM cells cultured with GM-CSF for the extended duration. These results indicate that BM cells in the extended culture with GM-CSF undergo 2 distinct steps of functional change; first, they lose the immunostimulatory capacity; and next, they gain the immunosuppressive ability.</P>

Dimethomorph의 제형에 따른 고추 역병 방제 효과

박정용,류연주,류나현,이용세 대구대학교 생명과학연구소 2004 생명과학연구 Vol.3 No.1

Dimethomorph(DMM) is an effective Oomycete fungicide useful for the control of late blight of potato and pepper by preventative sprays. Efficacy of five formulations, suspension concentrate (DM150), wettable powder (DM250) and three different dispersible concentrates (DM630, DM631, DM632), were investigated in their biological activity against phytophthora blight of red-pepper in vitro and in vivo. Their in vitro biological activities against mycelial growth, zoosporangial formation and zoosporangial germination of Phytophthora capsici were similar. The incidence of late blight on red pepper plants was progressively increased from June 11 to August 8 in untreated plants. However, in the case of DMM treated plants diseased plants, were increased after July 11. When compared with that on July 11, the incidence of late blight on July 18 in the treated plants except in DM630 were increased drastically. This increase were thought to be the result of flooding with more than 80 mm rainfall at July 15. The incidence of late blight on July 29 in the treated plants were increased compared with that on July 18. These also thought to be the result of flooding with more than 100 mm rainfall on July 23. All of the tested DMM formulations showed similar high control value against the late phytophthora blight on red pepper plants in the field test until July 11. However, after flooding the test field, DM630 showed the greatest disease control efficacy. DM250 showed relatively poor disease control efficacy in the test. Therefore more studies on the DM630 formulation are needed.

아크릴산 그라프트 폴리프로필렌 부직포로부터 아민형 음이온 교환체의 합성 및 PO₄-P의 제거특성

나춘기,박현주,정일현 7개 국립대학교 환경연구 논문집 공동발행 위원회 2003 공업기술연구 Vol.3 No.-

Aminated PP-g-AA that possess anionic exchangeable function was prepared by photoinduce grafting of acrylic acid onto polypropylene nonwoven fabric and followed chemical treatments using diethylene triamine coupled with aluminum chloride catalyst. Amination of grafted acrylic acid increased with increasing the reaction time and temperature of the aluminum catalysed amination procedure. Increasing the degree of amination, resulting in increasing the PO_(4)-P exchange capacity of aminated PP-g-AA and therefore, it reaches maximum value at the degree of amination as about 50-60%. Its value was much higher than those of conventional anion resins and activated carbon. Futhermore, aminated PP-g-AA nonwoven fabric could be regenerated in more than ten times by acid washing without any degradation of ion exchange capacity and physical properties.

하시모토 갑상선염을 동반한 갑상선 이형성증 1예

정현주,김예나,최영식,박요한 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.1

Thyroid hemiagenesis is a rare congenital anomaly, in which one thyroid lobe fails to develop. It is reported that thyroid hemiagenesis associated with thyroid diseases such as Graves' disease, Hashimoto's thyroiditis, colloidal goiter and thyroid follicular and papillary cancer. However, Thyroid hemiagenesis associated Hashimoto's thyroiditis haven't reported in Korea. A 31-year-old female patient was clinically hypothyroid with a left-sided goiter. Hemiagenesis of right thyroid lobe indicated on 99mTc pertechnete scan and later confirmed on ultrasonography. The authors report this case with literature review.

Capsaicin 사전 투여에 의한 흰쥐의 공격성 감소 및 자율적 체온조절의 결손

박순권,홍승길,나흥식,김현택 한국심리학회 한국심리학회지 생물 및 생리 Vol.7 No.1

capsaicin 사전 처치가 흰쥐의 공격성과 체온조절에 미치는 영향을 알아보았다. 생후 6주경에 capsaicin을 피하주사하였고, 완전히 성숙한 후에 공격성 및 체온조절 기능을 검사하였다. 실험 1의 공격성 검사 결과 capsaicin 처치동물들의 공격성은 통제동물보다 낮았는데, 이것은 선행 연구의 결과와 상반된다. 체온조절 기능을 알아본 실험 2에서는 capsaicin 처치동물들이 37℃ 및 40℃ 조건에서 과체온과 빠른 체온증가를 보여주었다. 이것은 출생 직후 또는 성숙한 후에 약물을 투여한 선행연구들과 일치되는 결과이다. 따라서 capsaicin이 체온조절에 미치는 영향은 투여 시기와 무관한 것 같다. 논의에서는 본 연구의 두 가지 결과를 시상하부와 관련시켜 해석하였다. The present study was designed to examine effects of capsaicin administration on aggressive behaviors and autonomic thermoregulation in rats. In six-week-old rat, capsaicin was injected subcutaneously on 4 consecutive days in increasing doses(20㎎/㎏, 30㎎/㎏, 30㎎/㎏, 50㎎/㎏) to total of 150㎎/㎏ of the drug. The controls were treated in the same way with vehicle alone. Two experments began six or eight weeks after the treatment. In experiment 1, isolation-induced agressive behaviors, scored a 10-min session in the dyadic situation, were significantly decreased by capsaicin pretreatment. This result was not in accord with the previous findings. In experiment 2, body temperature of the capsaicin-treated rats increased more than the control's at two amibient temperatures studied(37℃ and 40℃). Our result concerning thermoregulation supports the preceding studies that applied to the capsaicin-treated animals as neonate or adult. Thus, it is likely that the effect of capsaicin treatment on thermoregulation has nothing to do with the age of capsaicin injection. The capsaicin effects from this study were compared with hypothalamic lesion effects in the discussion part.

이중언어자에서 보인 두 형태의 실어증 : 증례보고 A Case Report

나해리,이정욱,박성민,박수열,권순용,이현정 대한치매학회 2004 Dementia and Neurocognitive Disorders Vol.3 No.2

Since many people in Korea know more than one language, bilingual aphasia is an important line of research in clinical and theoretical neurolinguistics. Nowadays we meet many people who speak other languages along with Korean, who are immigrants from foreign countries, especially from China. Differential recovery of language affected by an aphasic deficit is documented. In the present work, we introduced a patient with bilingual aphasia who showed different recovery patterns in Korean and Chinese. A 66-year-old man was presented with language disturbance and right hemiplegia. On past medical history, he had received a hematoma evacuation through burr-hole due to left basal ganglia hemorrhage. After the event, he showed nearly global aphasia in Korean, but he showed minimally preserved language function on comprehension and fluency in Chinese. This result suggests that aphasia in bilingual subject may show different pat-terns of recovery between two languages.