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RISS 인기검색어
- 검색키워드
- 저자 : MyoungKi Ahn (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
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Cross structured illumination for high speed high resolution line scanning confocal microscopy
Ahn, MyoungKi,Kim, Taejoong,Kim, YoungDuk,Gweon, DaeGab,Lee, Jun-Hee IOP Pub 2011 Measurement Science and Technology Vol.22 No.1
<P>Previous research presented the structured illumination confocal scanning microscope (SICSM) so as to improve the lateral resolution of the confocal microscope. However, the image acquisition speed of the SICSM is very slow and also an alignment error due to the mechanical rotation of a grating and a slit can easily occur. As a theoretical study, in this paper we propose a new SI method, the cross SI method, which improves lateral resolution and image acquisition speed. Performances of the conventional SI and the proposed SI methods are compared by analysis of the modulation transfer function. The proposed SI method shows similar lateral resolution and can shorten the image acquisition time compared to the conventional SI method. The cross structured illumination confocal microscope (CSICM) is combined with the cross SI pattern optics and the line scanning confocal microscope. We have introduced a 2-D diffractive grating, four linear polarizers and four cylindrical lenses in order to create the cross SI pattern. The effects of the cross SI pattern, intensity and visibility, on the system performance are analyzed. The CSICM has double the lateral resolution of the conventional microscope, an optical sectioning ability and a fast image acquisition speed.</P>
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Design and Fabrication of a Multi-modal Confocal Endo-Microscope for Biomedical Imaging
김영덕,MyoungKi Ahn,권대갑 한국광학회 2011 Current Optics and Photonics Vol.15 No.3
Optical microscopes are widely used for medical imaging these days, but biopsy is a lengthy process that causes many problems during the ex-vivo imaging procedure. The endo-microscope has been studied to increase accessibility to the human body and to get in-vivo images to use for medical diagnosis. This research proposes a multi-modal confocal endo-microscope for bio-medical imaging. We introduce the design process for a small endoscopic probe and a coupling mechanism for the probe to make the multi-modal confocal endo-microscope. The endoscopic probe was designed to decrease chromatic and spherical aberrations, which deteriorate the images obtained with the conventional GRIN lens. Fluorescence and reflectance images of various samples were obtained with the proposed endo-microscope. We evaluated the performance of the proposed endo-microscope by analyzing the acquired images, and demonstrate the possibilities of in-vivo medical imaging for early diagnosis.
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Song, Cheol,Ahn, MyoungKi,Gweon, DaeGab The Optical Society 2010 Optics express Vol.18 No.23
<P>We describe a polarization sensitive spectral domain optical coherence tomography technique based on a single camera spectrometer that includes a multiplexed custom grating, camera lenses, and a high-speed three-line CCD camera. Two orthogonally polarized beams could be separately taken by two lines of the camera as a result of vertically different incident angles. The system could provide the imaging capabilities of a full camera speed and increased measurable depth. The proposed optical coherence tomography system could make a distinction between the normal muscle and cancerous tissue from the chest of a DSred GFP mouse and the OCT images were compared with those of in vivo confocal microscopy.</P>
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