http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Myoung-Sug KIM(김명석),Ji-Woong JIN(진지웅),Sung-Hee JUNG(정승희),Jung-Soo SEO(서정수),Suhee HONG(홍수희) 한국수산해양교육학회 2015 水産海洋敎育硏究 Vol.27 No.5
Vibrio harveyi is a pathogenic marine bacterium causing systemic symptoms resulting in mass mortalities in fishes and shrimps in aquaculture. Outer membrane proteins(OMPs) are related to the pathogenicity and thus good targets for diagnosis and vaccination for Gram negative bacteria. Recently vaccination strategies using the OMPs have been suggested to control vibriosis in several fish species. In this study, we have isolated V. harveyi from diseased marine fishes from different regions of Korea and investigated genetic variations of four OMP genes including OmpK, OmpU, OmpV and OmpW. Consequently, OmpK and U genes could be divided into 3 subgroups of type I, II, III and type A, B, C, respectively, without any correlation with geographical regions and species while OmpV and W were highly homologous. OmpW gene of V. harveyi FP4138 was fully sequenced and predicted the deduced amino acid sequence to form β-barrel with hydrophobic channel. Indeed, the immunogenicity of recombinant OmpW produced in Escherichia coli was assessed by vaccinating flounder. As a result, the high antibody response with antibody titer of 4.2±0.7 and protection with relative percent survival of 60% against artificial infection of V. harveyi were demonstrated. This result indicates that OmpW is a virulence related factor and it can be a vaccine candidate to prevent a high mortality caused by V. harveyi infection in olive flounder, Paralichthys olivaceus.
( Myoung Sug Kim ),( Lyu Jin Jun ),( Soon Bum Shin ),( Myoung Ae Park ),( Sung Hee Jung ),( Kwang Il Kim ),( Kyung Ho Moon ),( Hyun Do Jeong ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.12
The full-length genes gyrB (2,415 bp), parC (2,277 bp), and parE (1,896 bp) in Edwardsiella tarda were cloned by PCR with degenerate primers based on the sequence of the respective quinolone resistance-determining region (QRDR), followed by elongation of 5` and 3` ends using cassette ligation-mediated PCR (CLMP). Analysis of the cloned genes revealed open reading frames (ORFs) encoding proteins of 804 (GyrB), 758 (ParC), and 631 (ParE) amino acids with conserved gyrase/topoisomerase features and motifs important for enzymatic function. The ORFs were preceded by putative promoters, ribosome binding sites, and inverted repeats with the potential to form cruciform structures for binding of DNA-binding proteins. When comparing the deduced amino acid sequences of E. tarda GyrB, ParC, and ParE with those of the corresponding proteins in other bacteria, they were found to be most closely related to Escherichia coil GyrB (87.6% identity), Klebsiella pneumoniae ParC (78.8% identity), and Salmonella Typhimurium ParE (89.5% identity), respectively. The two topoisomerase genes, parC and parE, were found to be contiguous on the E. tarda chromosome. All 18 quinolone-resistant isolates obtained from Korea thus far did not contain subunit alternations apart from a substitution in GyrA (Ser8→Arg). However, an alteration in the QRDR of ParC (Ser84→Ile) following an amino acid substitution in GyrA (Asp87→Gly) was detected in E. tarda mutants selected in vitro at 8 μg/ml ciprofloxacin (CIP). A mutant with a GyrB (Ser464→Leu) and GyrA (Asp87→Gly) substitution did not show a significant increase in the minimum inhibitory concentration (MIC) of CIP. None of the in vitro mutants exhibited mutations in parE. Thus, gyrA and parC should be considered to be the primary and secondary targets, respectively, of quinolones in E. tarda.
서정수 ( Jung Soo Seo ),전은지 ( Eun Ji Jun ),정승희 ( Sung Hee Jung ),김명석 ( Myoung Sug Kim ),박명애 ( Myoung Ae Park ),이철호 ( Chul Ho Lee ),한명철 ( Myoung Chul Han ),김진우 ( Jin Woo Kim ),지보영 ( Bo Young Jee ) 한국어병학회 2010 한국어병학회지 Vol.23 No.1
The infestation status of anisakid type larvae was investigated in migrating chum salmon (Oncorhynchus keta), with different condition (captured area, sex, body portion) during 2006~2008. The mean infection number of anisakid larvae per individual female and male fish captured from Namdae river was 98±27, 103±27, respectively. The mean infection number of anisakid larvae per individual female and male fish captured from the coastal area of Yangyang was 63±18 and 108±17, respectively. The anisakid larvae were mainly found in abdominal muscles (85%) but only a little in the visceral portion. Two types of anisakid larvae (A. simplex, Contracaecum type) were identified but other anisakid larvae were not detected. To investigate the effect of storing temperature on the viability of anisakid larvae, the section of abdominal muscle were stored at different temperature (room temperature, 4℃, -20℃, -80℃). As a result, it was necessary to store at -20℃ for more than 6 hrs to kill the larvae. The present results revealed that chum salmon caught in Korea are heavily infected with anisakid larvae, mainly in the abdominal muscle, and A. simplex was dominantly found in this study.
Complete genome sequence and comparative genome analysis of Streptococcus parauberis KCTC11980
Seo, Jung Soo,Kwon, Mun Gyeong,Hwang, Jee Youn,Jung, Sung Hee,Han, Hyun Ja,Kim, Myoung Sug,Do, Jeong-Wan,Park, Myoung Ae,Kim, Dong-Wook,Cho, Wang Sik,Lee, Kyungho Springer-Verlag 2015 Genes & Genomics Vol.37 No.11
양식 넙치, Paralichthys olivaceus에서 분리된 Edwardsiella tarda, Vibrio spp., Streptococcus spp.의 항균제 내성 경향
김명석 ( Myoung Sug Kim ),서정수 ( Jung Soo Seo ),박명애 ( Myoung Ae Park ),조지영 ( Ji Young Cho ),황지연 ( Jee Youn Hwang ),권문경 ( Mun Gyeong Kwon ),정승희 ( Sung Hee Jung ) 한국어병학회 2010 한국어병학회지 Vol.23 No.1
In this study, we carried out research on the level of single and multi-drug resistance of bacteria isolated from cultured flounder Paralichthys olivaceus. One hundred sixty one bacteria were isolated from cultured olive flounder Paralichthys olivaceus in Korea and the isolates consisted of Edwardsiella tarda (n=32), Vibrio ichthyoenteri (n=37), Vibrio spp. (n=54), Streptococcus parauberis (n=28) and Streptococcus spp. (n=10). These E. tarda isolates were highly resistant in the order of tetracycline (84.4%) and oxolinic acid (71.9%). V. ichthyoenteri and Vibrio spp. showed resistance ampicillin (94.6% and 81.5%) and tetracycline (56.8% and 42.6%). S. parauberis isolates were resistant ampicillin (57.1%), tetracycline (57.1%) and erythromycin (35.7%). Of the isolates, 84.4% of E. tarda, 73.0% of V. ichthyoenteri, 57.4% of Vibrio spp., 42.8% of S. parauberis and 70.0% of Streptococcus spp. isolates exhibited multi-drug resistance against more than two antibiotics.
양식 넙치 (Paralichthys olivaceus) 에서 분리된 Edwardsiella tarda에 대한 항균제 MIC 값 분포
김명석 ( Myoung Sug Kim ),조지영 ( Ji Young Cho ),서정수 ( Jung Soo Seo ),정승희 ( Sung Hee Jung ),최혜승 ( Hye Sung Choi ),박명애 ( Myoung Ae Park ) 한국어병학회 2012 한국어병학회지 Vol.25 No.3
The minimum inhibitory concentration (MIC) of eight antibiotics against Edwardsiella tarda 49 strains isolated from olive flounder (Paralichthys olivaceus) was determined by the broth microdilution method. E. tarda showed 38.8% and 61.2% resistance to ampicillin and amoxicillin. Both resistance rates of E. tarda were 4.1% against ciprofloxacin and norfloxacin. The MIC50 values of oxytetracycline, amoxicillin and oxolinic acid were 64 ㎍/㎖, 32 ㎍/㎖and 4 ㎍/㎖. The MIC50 value to ciprofloxacin was 0.25 ㎍/㎖which was lowest among eight antibiotics tested.
Kim, Myoung Sug,Jung, Sung Hee,Hong, Suhee,Jeong, Hyun Do The Korean Society of Fisheries and Aquatic Scienc 2015 Fisheries and Aquatic Sciences Vol.18 No.4
Biotyping of Vibrio vulnificus strains isolated from marine environments along the south coast of Korea showed that the majority of the isolates (94.7%) belonged to biotype 1 and the remaining isolates (5.3%) belonged to biotype 2. Analysis of 16S rRNA V. vulnificus strains isolated from marine environments using a multiplex polymerase chain reaction (PCR) revealed that 78.7% were type A and 21.3% were type B. Random amplified polymorphic DNA (RAPD) was used to analyze the genomic differences in V. vulnificus among the biotype 2 strains isolated from marine environments (newly isolated strains group) and reference strains obtained from infected eels (reference strains group). The two groups had distinctly different profiles of the amplicons produced from RAPD. Additionally, biochemical comparison of these strains revealed that all four strains isolated from marine environments differed from the strains isolated from eels in their ability to promote D-mannitol fermentation. Two (NH 1 and NH 2) out of four isolates of biotype 2 from marine environments showed pathogenicity in eels Anguilla japonica in a challenge test. These isolates did not agglutinate with antisera against V. vulnificus NCIMB 2137 (serovar E), ATCC 27562 (non-serovar E), and ATCC 33816 (atypical serovar E).