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      • KCI등재

        Optical Technique to Measure Five-Degree-of-Freedom Error Motions for a High-Speed Microspindle

        Hiroshi Murakami1,Norio Kawagoishi,Eiji Kondo,Akira Kodama 한국정밀공학회 2010 International Journal of Precision Engineering and Vol. No.

        We present an optical technique to measure five-degree-of-freedom error motions of a high-speed microspindle. The measurement system consists of a rod lens, a ball lens, four divided laser beams, and multiple divided photodiodes. When the spindle rotates with its concomitant rotation errors, the rod and ball lenses, which are mounted to the chuck of the spindle, are displaced, and this displacement is measured using an optical technique. For this study, we decide the design parameters of the optical system using ray tracing, fabricate a prototype of the measurement system, and evaluate it experimentally. The results show that the measurement system has a resolution of 5 nm and can be used to evaluate high-speed microspindle rotation errors.

      • The Initial Enzyme for Glycosylphosphatidylinositol Biosynthesis Requires PIG-Y, a Seventh Component

        Murakami, Yoshiko,Siripanyaphinyo, Uamporn,Hong, Yeongjin,Tashima, Yuko,Maeda, Yusuke,Kinoshita, Taroh American Society for Cell Biology 2005 Molecular biology of the cell Vol.16 No.11

        <P>Biosynthesis of glycosylphosphatidylinositol (GPI) is initiated by an unusually complex GPI-N-acetylglucosaminyltransferase (GPI-GnT) consisting of at least six proteins. Here, we report that human GPI-GnT requires another component, termed PIG-Y, a 71 amino acid protein with two transmembrane domains. The Burkitt lymphoma cell line Daudi, severely defective in the surface expression of GPI-anchored proteins, was a null mutant of PIG-Y. A complex of six components was formed without PIG-Y. PIG-Y appeared to be directly associated with PIG-A, implying that PIG-Y is the key molecule that regulates GPI-GnT activity by binding directly to the catalytic subunit PIG-A. PIG-Y is probably homologous to yeast Eri1p, a component of GPI-GnT. We did not obtain evidence for a functional linkage between GPI-GnT and ras GTPases in mammalian cells as has been reported for yeast cells. A single transcript encoded PIG-Y and, to its 5′ side, another protein PreY that has homologues in a wide range of organisms and is characterized by a conserved domain termed DUF343. These two proteins are translated from one mRNA by leaky scanning of the PreY initiation site.</P>

      • SCIESCOPUSKCI등재

        Photosynthetic Performances of Temperate Sargassum and Kelp Species Growing in the Same Habitat

        Murakami, Hiroshige,Serisawa, Yukihiko,Kurashima, Akira,Yokohama, Yasutsugu The Korean Society of Phycology 2004 ALGAE Vol.19 No.3

        Characteristics of photosynthesis-light and photosynthesis-temperature relationships were seasonally compared among 4 species, two temperate Sargassurn (S. ringgoldianum and S. spathulophyllum) and two temperate kelp (Ecklonia cava and Eisenia bicyclis), growing in the same habitat in Oura Bay, Shimoda, central Japan. The photosynthesis-irradiance (P-I) curve of each species at the in situ temperature and the photosynthesis-temperature (P-T) curve at near saturation irradiance (400μmol·m^(-2)·s^(-1)) were determined by using differential gas-volumeters. Maximum photosynthetic rates (Prnax) for P-I curves of the two Sargassurn species were higher from summer to autumn than from winter to spring, while those of the two kelp species showed little difference among seasons. Net photosynthetic rates (Pn) at 100-400μmol·m^(-2)·s^(-1) of the Sargassurn species were higher than those of the kelp species in autumn, spring and summer, while in winter the rates were about the same between the Sargassurn and kelp species. Among seasons, the light saturation index (1k) values, dark respiration rates and light compensation points of Sargassurn species differed more than those of kelp species. Optimum temperature for P-T curves of Sargassurn species was 29℃ and that of kelp species was 27℃ in summer, while in winter the former was 27℃ and the latter was 25℃. Pn at 400 μmol·m^(-2)·s^(-1) at 10-33℃ of Sargassurn species were considerably higher than those of kelp species from spring to summer, while from autumn to winter the rates at 5-27℃ were about the same between the two. At supra-optimum temperatures, Pn of kelp species decreased more sharply than those of Sargassurn species in each season. These facts indicate that the two temperate Sargassurn species have a higher potential photosynthetic performance under warmer conditions than the two temperate kelp species even though they grow in the same habitat.

      • A Novel Chlorophyll d-containing Organism: Discovery and its Significance

        Murakami, Akio,Kawai, Hiroshi,Adachi, Kyoko,Sakawa, Takahiro,Miyashita, Hideaki,Mimuro, Mamoru Korean Society of Photoscience 2002 Journal of Photosciences Vol.9 No.2

        Chlorophyll (Chi) d was assigned to an antenna pigment of red algae in 1943, but its presence and function in red algae have not been necessarily clear for a long time. In 1996, it was shown that Chi d functioned as a major antenna pigment in a peculiar oxygenic photosynthetic prokaryote, Acaryochloris marina, isolated as a symbiont of a colonial ascidian from coral reefs. This finding evoked the necessity for reexamination of the presence and function of Chi d in red algae. We found Chi d in methanol-extract from several marine red algae, and the relative content was high in one species, Ahnfeltiopsis flabelliformis. Absorption and fluorescence spectra, HPLC analysis, and NMR and mass spectroscopy characterized Chi d extracted from the red algal thalli, and those were essentially identical to those of Chi d isolated from A. marina. However, micro-spectrophotometric analysis suggested that Chi d was not an actual constituent of the red algae but came from epiphyte(s) attached to surface of red algal thalli.

      • SCIESCOPUSKCI등재

        Effects of Functional Oils on Coccidiosis and Apparent Metabolizable Energy in Broiler Chickens

        Murakami, A.E.,Eyng, C.,Torrent, J. Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.7

        The objective of the present study was to investigate the effects of a mixture of functional oils (Essential, Oligo Basics Agroind. Ltda) on performance response of chickens challenged with coccidiosis and the determination of apparent metabolizable energy (AME), nitrogen-corrected apparent metabolizable energy (AMEn), the coefficients of protein and ether extract digestibility and intestinal morphology of broilers fed with diets containing Essential. In Exp. 1, a completely randomized design (CRD) was used, with one control diet without Essential inclusion with coccidiosis (Eimeria acervulina, Eimeria maxima, and Eimeria tenella) challenged birds and two different inclusion rates of Essential (1.5 kg/ton and 2 kg/ton) with coccidiosis-challenged and non-challenged birds for each inclusion rate, using 10 replicates and 50 birds per experimental unit. After 7 d of coccidiosis challenge, the livability was approximately 10% lower (p<0.05) for the control group. Intestinal lesion scores were lower (p<0.05) in the anterior intestine and the cecum for the chickens supplemented. Feed efficiency and growth rate were improved in birds supplemented with Essential (p<0.05) before the coccidiosis challenge and during the first 7 d post infection. In Exp. 2, a CRD was used, with one control diet without Essential inclusion and one diet with inclusion of Essential (1.5 kg/ton), using nine replications and 33 chicks per pen. The diets with Essential yielded approximately 4% higher AME (p = 0.003) and $AME_n$ (p = 0.001). Essential supplementation increased villus height in the jejunum on d 14 (p<0.05). Villus height:crypt depth ratio for the supplemented birds was larger (p<0.05) in the jejunum on d 7, larger (p<0.05) in the jejunum and ileum on d 14. In conclusion, these functional oils improved the energy utilization and the livability and decreased lesions caused by coccidiosis in supplemented birds.

      • SCIESCOPUS

        Setting the scene: CFD and symposium overview

        Murakami, Shuzo Techno-Press 2002 Wind and Structures, An International Journal (WAS Vol.5 No.2

        The present situation of CWE(Computational Wind Engineering) and the papers presented to the CWE 2000 Symposium are reviewed from the following viewpoints; 1) topics treated, 2) utilization of commercial code (software), 3) incompleteness of CWE, 4) remaining research subjects, 5) prediction accuracy, 6) new fields of CWE application, etc. Firstly, new tendencies within CWE applications are indicated. Next, the over-attention being given to the application field and the lack of attention to fundamental problems, including prediction error analysis, are pointed out. Lastly, the future trends of CFD (Computational Fluid Dynamics) applications to wind engineering design are discussed.

      • Development of New SCADA System for 500kV Substations

        Murakami, Juri,Kimoto, Hirofumi,Ono, Manabu,Kasahara, Kuniyoshi,Hayasaka, Yasushi,Ueno, Yasuhide 대한전기학회 2013 The Journal of International Council on Electrical Vol.3 No.1

        We developed a new SCADA system for 500-kV substations. To apply existing LAN-based SCADA system for unmanned 275-kV or 77-kV substations, we had to develop three functions. Firstly, the new SCADA system enables that 500-kV substation is supervised and controlled from multiple operating sites. Secondly, the database of the new SCADA system can be downloaded the maintenance data from the SCADA system for 500-kV control center via an IP network. Finally, the new SCADA system has supervisory control capability for manned 500-kV substations. In this paper, we describe these functional over view of the new SCADA system. Currently, 500-kV substations are manned substations. And these substations are supervised and controlled by human operators on sites. Using the new SCADA system, 500-kV substations will be realized remote supervisory control from the 500-kV control center a few years later. Therefore, manned 500-kV substations will convert into unmanned substations.

      • Adhesive Behaviors of the Aluminum Alloy-Based CrN and TiN Coating Films for Ocean Plant

        Murakami, Ri-Ichi,Yahya, Syed Qamma Bin Korean Society of Ocean Engineers 2012 International journal of ocean system engineering Vol.2 No.2

        In the present study, TiN and CrN films were coated by arc ion plating equipment onto aluminum alloy substrate, A2024. The film thickness was about 4.65 ${\mu}m$. TiN and CrN films were analyzed by X-ray diffraction and energy dispersive X-ray equipments. The Young's modulus and the micro-Vickers hardness of aluminum substrate were modified by the ceramic film coatings. The difference in Young's modulus between substrate and coating film would affect on the wear resistance. The critical load, Lc, was 75.8 N for TiN and 85.5 N for CrN. It indicated from the observation of optical micrographs for TiN and CrN films that lots of cracks widely propagated toward the both sides of scratch track in the early stage of MODE I. TiN film began to delaminate completely at MODE II stage. The substrate was finally glittered at MODE III stage. For CrN film, a few crack can be observed at MODE I stage. The delamination of film was not still occurred at MODE II and then was happened at MODE III. This agrees with critical load measurement which the adhesive strength was greater for CrN film than for TiN film. Consequently, it was difficult for CrN to delaminate because the adhesive strength was excellent against Al substrate. The wear process, which the film adheres and the ball transfers, could be enhanced because of the increase in loading. The wear weight of ball was less for CrN than for TiN. This means that the wear damage of ball was greater for TiN than for CrN film. It is also obvious that it was difficult to delaminate because the CrN coating film has high toughness. The coefficient of friction was less for CrN coating film than for TiN film.

      • SCOPUSKCI등재

        Changes in Air Temperature and Its Relation to Ambulance Transports Due to Heat Stroke in All 47 Prefectures of Japan

        Murakami, Shoko,Miyatake, Nobuyuki,Sakano, Noriko The Korean Society for Preventive Medicine 2012 Journal of Preventive Medicine and Public Health Vol.45 No.5

        Objectives: Changes in air temperature and its relation to ambulance transports due to heat stroke in all 47 prefectures, in Japan were evaluated. Methods: Data on air temperature were obtained from the Japanese Meteorological Agency. Data on ambulance transports due to heat stroke was directly obtained from the Fire and Disaster Management Agency, Japan. We also used the number of deaths due to heat stroke from the Ministry of Health, Labour and Welfare, Japan, and population data from the Ministry of Internal Affairs and Communications. Chronological changes in parameters of air temperature were analyzed. In addition, the relation between air temperature and ambulance transports due to heat stroke in August 2010 was also evaluated by using an ecological study. Results: Positive and significant changes in the parameters of air temperature that is, the mean air temperature, mean of the highest air temperature, and mean of the lowest air temperature were noted in all 47 prefectures. In addition, changes in air temperature were accelerated when adjusted for observation years. Ambulance transports due to heat stroke was significantly correlated with air temperature in the ecological study. The highest air temperature was significantly linked to ambulance transports due to heat stroke, especially in elderly subjects. Conclusions: Global warming was demonstrated in all 47 prefectures in Japan. In addition, the higher air temperature was closely associated with higher ambulance transports due to heat stroke in Japan.

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