Disaster risks, economic growth, and dynamic mitigation policy

Muneta Yokomatsu,Kisoshi Kobayashi 환경독성보건학회 2004 한국독성학회 심포지움 및 학술발표회 Vol.- No.-

Twenty-Year Experience of a Double-Bundle Anterior Cruciate Ligament Reconstruction

Takeshi Muneta 대한정형외과학회 2015 Clinics in Orthopedic Surgery Vol.7 No.2

Double-bundle (DB) anterior cruciate ligament (ACL) reconstruction using a four-strand semitendinosus tendon was started in our department in July 1994. The motivation for starting the procedure was that the EndoButton with an inside-out procedure instrument became available in Japan. A review article of our DB ACL reconstruction procedure was summarized for the twentieth anniversary of the surgical procedure. Initial tension setting of the two grafts was changed in the first 8 years to achieve better stability during DB ACL reconstruction. A randomized clinical trial (RCT) was started in July 2002 to clarify superiority of the DB procedure to single-bundle (SB) reconstruction under the concept of anatomic reconstruction. Several anatomic studies were performed to describe normal ACL anatomy, which is essential for realizing anatomic reconstruction. A remnant-preserving technique would be an additional option for our DB procedure to improve reconstruction outcomes. Thus, a new remnant-preserving DB procedure was started in 2012. The reproducibility of the new procedure was investigated using three-dimensional computed tomography images. More complex procedures were performed using a transtibial technique and EndoButtons. Initial tension balancing between the two grafts was important for a better outcome. Superiority of knee stability after the DB compared to that after the SB procedure was clarified by the RCT. However, no patient consensus has been reached on any subjective advantage to the DB procedure. Studies of normal ACL anatomy have left questions unresolved regarding where the two tunnels should be created for direct and indirect insertions based on normal anatomy. A new remnant-preserving DB ACL procedure has been practiced. The procedure was more reproducible with respect to creating the femoral tunnel. DB ACL reconstruction using a semitendinosus tendon is an attractive option when pursuing a better outcome for patients.

INSTAGRAM IMPACT ON DESTINATION MANAGEMENT ORGANIZATION

Maria Elena Aramendia-Muneta,Cristina Olarte-Pascual,Andrea Ollo-L?pez 글로벌지식마케팅경영학회 2018 Global Marketing Conference Vol.2018 No.07

The purpose of this study is to find out the key to success of the photographs in Instagram through content for tourist destination. While there are several studies in the tourism industry about the impact of Social Networks such as Facebook or virtual collaborative communities like TripAdvisor, there are very few studies about Instagram (Hanan & Putit, 2014). This study focuses on “Beautiful Destinations” as the leader on Instagram and an example of destination management organization (DMO), where they have more than 3,100 publications and 4 million followers in 180 countries, becoming the world’s largest travel influencer on Instagram. The dependent variables are taking from several studies (Dagostar & Isotalo, 1992; Stabler, 1987; Timothy & Groves, 2001). Ordinary least squares models were estimated to assess the relationship of characteristics of the photo with the number of likes among photos published on Instagram. If a photograph has people, water, took during afternoon, self-centric and other centric have effects in the number of likes and comments. While if a destination has the purpose of the engagement of the consumers with several comments, people appearance has a positive impact in the number of comments and if the pictures is doing in the afternoon has a negative impact. By analyzing the contents of information provided by the uploaded photographs, this study provides clues for destination in order to enhance the engagement with potential customer and users of Instagram.

IFNImmunosuppression of xenograft rejection in porcine kidney PK15 cells by porcine IL-18

최윤실,김영관,심정현,김은미,강형식,윤도영,Yoshihiro Muneta,명평근 생화학분자생물학회 2006 Experimental and molecular medicine Vol.38 No.5

Xenotransplantation, the transplantation of cells, tissues or organs between individuals of different species, would resolve the current shortage of organs, but rejection remains the major hurdle to successful xenotransplantation. In the present study, we analyzed mixed lymphocyte reactions (MLRs) and used 51Cr release assays in order to identify the proliferation and expansion of mouse CD8+cytotoxic T lymphocyte cells against PK15, PK15/pIL-18 or PK15/mIL-18 cells. In addition, we identified T cell populations in mouse splenocytes and lymph node cells using two-color flow cytometry. It was found that the CD8+ T cells of xenograft recipients proliferated extensively and that the survival rates of populations of PK15/mIL-18 or PK15/pIL-18 cells were higher than untransfected controls. Moreover, CD3+T cells were increased in mice injected with PK15 cells or PK15/pIL-18 cells but PK15/pIL-18 cell numbers were lower in lymph nodes than untransfected controls. CD8+ T cells numbers were reduced in the lymph nodes of PK15/pIL-18 injected mice. These results suggest that porcine IL-18 regulates anti-pig cellular rejection in C57BL/6 mice.

A Map-based Cloning Approach for the Identification of a Low Temperature Sensitive Gene sy-2 in Chilli pepper (Capsicum chinense)

L iLiu,Jin-Ho Kang,Yeong Deuk Jo,Sota Koeda,Munetaka Hosokawa,Doil Choi,Byoung-Cheorl Kang 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

sy-2 (Seychelles-2) is a temperature sensitive mutant of Capsicum chinense and native to Seychelles Island in Africa. Previously we showed that sy-2 leaves were irregularly shaped and defective in chlorophyll development at temperatures lower than 24℃. A segregation test revealed that the sy-2 gene is controlled by a single recessive gene. To identify the sy-2 gene, we performed a map-based cloning approach using a total of 1,010 F2 plants derived from crossing sy-2 and the wild type C. chinense ‘No.3341’. sy-2 gene is located on chromosome 1, 0.3 cM and 0.1cM away from cosII markers C2_At4g29120 and C2_At1g09070, respectively. The tomato genome sequence between those two markers was compared with pepper genome sequence. We found three of pepper scaffold sequences in this region. We developed seven ingle nucleotide polymorphism (SNP) markers on the pepper scaffold sequences, among which five SNP markers were co-segregated with sy-2. To fill the gap between the scaffolds which contains co-segregating markers, we screened a bacterial artificial chromosome (BAC) library, and end-sequences of total of 22 AC clones were i. We found that five clones were overlapped to cover the gap. We fully sequenced four AC clones and found that the physical distance between C2_At4g29120 and C2_At1g09070 is 343kb. This region contains 70 putative genes such as HSP90-like ATPase family proteins, lipid-transfer proteins, calmodulin-domain protein kinases, and zinc finger proteins (ZFPs). To identify the sy-2 gene, we performed RT-PCR and found that a ZFP-like gene is differentially expressed between WT and sy-2 leaves. This result suggests that the ZFP-like gene is a strong candidate for the sy-2 gene. We are currently characterizing this candidate gene.

Humeral Insertion of the Supraspinatus and Infraspinatus

Tomoyuki Mochizuki,Hiroyuki Sugaya,Mari Uomizu,Kazuhiko Maeda,Keisuke Matsuki,Ichiro Sekiya,Takeshi Muneta,Keiichi Akita 대한견주관절의학회 2009 대한견주관절학회 학술대회논문집 Vol.2009 No.5

Immunosuppression of xenograft rejection in porcine kidney PK15 cells by porcine IL-18

Choi, Yon Sil,Kim, Young Kwan,Shim, Jung Hyun,Kim, Eun Mi,Kang, Hyung Sik,Yoon, Do Young,Muneta, Yoshihiro,Myung, Pyung Keun 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

Xenotransplantation, the transplantation of cells, tissues or organs between individuals of different species, would resolve the current shortage of organs, but rejection remains the major hurdle to successful xenotransplantation. In the present study, we analyzed mixed lymphocyte reactions (MLRs) and used (51)^Cr release assays in order to identify the proliferation and expansion of mouse CD8^(+) cytotoxic T lymphocyte cells against PK15, PK15/pIL-18 or PK15/mlL-18 cells. In addition, we identified T cell populations in mouse splenocytes and lymph node cells using two-color flow cytometry. It was found that the CD8^(+) T cells of xenograft recipients proliferated extensively and that the survival rates of populations of PK15/mlL-18 or PK15/plL-18 cells were higher than untransfected controls. Moreover, CD3^(+) T cells were Increased in mice injected with PK15 cells or PK15/plL-18 cells but PK15/plL-18 cell numbers were lower In lymph nodes than untransfected controls. CD8^(+) T cells numbers were reduced in the lymph nodes of PK15/plL-18 injected mice. These results suggest that porcine lL-18 regulates anti-pig cellular rejection In C57BL/6 mice.