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Prevalence of antibody to and DNA of Lawsonia intracellularis in samples from wild animals in Korea
Hossain, M. D. Mukter,Oh, Yeonsu,Cho, Ho-Seong WILDLIFE DISEASE ASSOCIATION 2016 JOURNAL OF WILDLIFE DISEASES Vol.52 No.4
<P>We evaluated the prevalence of Lawsonia intracellularis infection in three wild animal species in Korea; the Korean water deer (Hydropotes inermis), Siberian roe deer (Capreolus pygargus), and raccoon dogs (Nyctereutes procyonoides). We collected 136 sera and 109 fecal samples from individuals in 10 Wildlife Rescue and Conservation Centers. Serum samples were tested for anti-L. intracellularis antibodies using a blocking enzyme-linked immunosorbent assay (bELISA), and fecal samples were subjected to a real-time PCR assay for L. intracellularis. Thirty-five (25.7%) sera and 36 (33.0%) fecal samples were positive. We found a higher proportion of positive sera (64.7%, chi(2) = 15.439, P< 0.01) and feces (58.8%, chi(2) = 6.126, P< 0.05) in raccoon dogs (chi(2) = 11.855, P< 0.01) than in the other species (20% positive sera and 29% positive feces in Korean water deer; 20% positive sera and 25% positive feces in Siberian roe deer). Our data indicate infection by L. intracellularis in Korean water deer, Siberian roe deer, and raccoon dogs throughout the country. It is imperative to know whether these infected animal species are natural hosts for L. intracellularis in addition to domestic pigs (Sus scrofa domesticus).</P>
( Sung-hyun Moon ),( Md Mukter Hossain ),( Yeonsu Oh ),( Ho-seong Cho ) 한국동물위생학회 2016 한국동물위생학회지 (KOJVS) Vol.39 No.1
In this study, Bacillus licheniformis which has been used as probiotics was isolated from Korean traditional fermented soybean. A total of 69 strains were presumptively identified as B. licheniformis by phenotypic methods. Based on PCR amplification and 16S rRNA gene sequencing, the multilocus sequence typing of gyrA and rpoB, followed by phylogenetic analysis was performed. The isolates were distinctly differentiated and found to be closely related to B. amyloliquefaciens, B. subtilis, and B. aerius. The partial 16S rRNA gene sequences of those strains matched those of B. sonorensis (97%) and B. aerius (98%) in the phylogenetic tree. In contrast, multilocus phylogenetic analysis (MLPA) showed that only 61 (86.9%) out of 69 strains were B. licheniformis. The rest of those strains were found to be B. subtilis (5.8%), B. amyloliquefaciens (2.9%), and B. sonorensis (2.9%), respectively. Therefore, our results suggested that since the 16S rRNA gene sequencing alone was not sufficient to compare and discriminate closely related lineages of Bacillus spp., it was required to analyze the MLPA simultaneously to avoid any misleading phenotype-based grouping of these closely related species.
Moon, Sung-Hyun,Hossain, Md Mukter,Oh, Yeonsu,Cho, Ho-Seong The Korean Society of Veterinary Service 2016 韓國家畜衛生學會誌 Vol.39 No.1
In this study, Bacillus licheniformis which has been used as probiotics was isolated from Korean traditional fermented soybean. A total of 69 strains were presumptively identified as B. licheniformis by phenotypic methods. Based on PCR amplification and 16S rRNA gene sequencing, the multilocus sequence typing of gyrA and rpoB, followed by phylogenetic analysis was performed. The isolates were distinctly differentiated and found to be closely related to B. amyloliquefaciens, B. subtilis, and B. aerius. The partial 16S rRNA gene sequences of those strains matched those of B. sonorensis (97%) and B. aerius (98%) in the phylogenetic tree. In contrast, multilocus phylogenetic analysis (MLPA) showed that only 61 (86.9%) out of 69 strains were B. licheniformis. The rest of those strains were found to be B. subtilis (5.8%), B. amyloliquefaciens (2.9%), and B. sonorensis (2.9%), respectively. Therefore, our results suggested that since the 16S rRNA gene sequencing alone was not sufficient to compare and discriminate closely related lineages of Bacillus spp., it was required to analyze the MLPA simultaneously to avoid any misleading phenotype-based grouping of these closely related species.
Serological prevalence of brucellosis of cattle in selected dairy farms in Bangladesh
Hassan, Abdullah Al,Uddin, M. Bashir,Islam, Md. Rafiqul,Cho, Ho-Seong,Hossain, Md. Mukter The Korean Society of Veterinary Science 2014 大韓獸醫學會誌 Vol.54 No.4
This study was conducted to investigate the status of brucellosis in dairy cattle from five selected dairy farms in the Mohammadpur Beribadh area of Bangladesh. A cross-sectional study was carried out from October 2010 to March 2011 in which a total of 334 serum samples from cattle in five herds were screened by the Rose-Bengal plate-agglutination test (RBPT) and the positives were confirmed using an indirect enzyme-linked immunosorbent assay (I-ELISA). A structured questionnaire was used to collect epidemiological information describing the animals. Overall, 4.20% of the animals were RBPT positive, while subsequent confirmatory tests with I-ELISA revealed that the overall animal-level prevalence derived from the samples was 1.20%. Additionally, the prevalence was relatively higher in females than in males. A significant association was found between abortion, age of the animals, and the occurrence of brucellosis (p < 0.05). Considering the overall low prevalence of brucellosis in the selected farms in the present study, a brucellosis eradication program for dairy farms using a test-and-slaughter policy would be possible.
Byung-Yong, PARK,Kwan-Seob, SHIM,Won-Il, KIM,Md Mukter, HOSSAIN,Bumseok, KIM,Jungkee, KWON,Choi-Kyu, PARK,Sung-Jin, CHO,Inho, JO,Ho-Seong, CHO Walter de Gruyter GmbH 2015 Acta veterinaria (Belgrade) Vol.65 No.1
<B>Abstract</B><P>A simple and rapid real-time loop-mediated isothermal amplification (LAMP) assay designed to detect <I>Lawsonia</I> (<I>L</I>.) <I>intracellularis</I>, an important bacteria causing proliferative enteropathy in pigs. A set of four primers targeting the ubiquinone/menaquinone biosynthesis methylase (<I>ubi</I>E) gene was designed for the LAMP reaction. Additionally, serial 10-fold dilutions of cultured <I>L. intracellularis</I> and spiked feces were also used for the optimization of real-time LAMP. The lower limit of the linear range of the assay in <I>L. intracellularis</I> was 1.0 × 10<SUP>0</SUP><I>L</I>. <I>intracellularis</I>. Real-time LAMP was 10 and 100 times more sensitive than real-time PCR and conventional PCR detection methods, respectively. Based on testing of 213 porcine fecal samples using real-time LAMP, realtime PCR and PCR, the agreement quotients of real-time LAMP with conventional PCR and with real-time PCR were 0.77 and 0.95, respectively. This study demonstrated that real-time LAMP was a powerful tool for the rapid and sensitive detection of <I>L</I>. <I>intracellularis</I> in porcine fecal samples.</P>
돼지생식기호흡기증후군바이러스(PRRSV)의 전장 유전체염기서열(whole-genome sequencing) 분석을 위한 차세대 염기서열 분석법의 활용
문성현 ( Sung-hyun Moon ),아미나카툰 ( Amina Khatun ),김원일 ( Won-il Kim ),후세인엠디묵터 ( Md Mukter Hossain ),오연수 ( Yeon Su Oh ),조호성 ( Ho-seong Cho ) 한국동물위생학회 2016 한국동물위생학회지 (KOJVS) Vol.39 No.1
In the present study, fast and robust methods for the next generation sequencing (NGS) were developed for analysis of PRRSV full genome sequences, which is a positive sensed RNA virus with a high degree of genetic variability among isolates. Two strains of PRRSVs (VR2332 and VR2332-R) which have been maintained in our laboratory were used to validate our methods and to compare with the sequence registered in GenBank (GenBank accession no. EF536003). The results suggested that both of strains had 100% coverage with the reference; the VR2332 had the coverage depth from minimum 3 to maximum 23,012, for the VR2332-R from minimum 3 to maximum 41,348, and 22,712 as an average depth. Genomic data produced from the massive sequencing capacities of the NGS have enabled the study of PRRSV at an unprecedented rate and details. Unlike conventional sequence methods which require the knowledge of conserved regions, the NGS allows de novo assembly of the full viral genomes. Therefore, our results suggested that these methods using the NGS massively facilitate the generation of more full genome PRRSV sequences locally as well as nationally in regard of saving time and cost.