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Nutshera Kittithanesuan,Muenduen Phisalaphong 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.3
To promote its performance during acetonebutanol- ethanol (ABE) fermentation, Clostridium acetobutylicum (ATCC 824) was immobilized on a thin-shell silk cocoon (TSC). As a residual from the silk industry, TSC offers a cheap, biocompatible support material. The adsorbed C. acetobutylicum cells digested the TSCs into amino acids as a nitrogen source. It was shown that TSC might promote the phase shift to acetone in the ABE fermentation. At an initial reducing sugar concentration of 90 g/L, the ABE productivity of the immobilized cell culture on TSC (IC-TSC) in batch fermentation was 0.18 g/L/h, and the solvent mixture comprised 6.1 g/L acetone, 15.9 g/L butanol, and 1.9 g/L ethanol. Repeated 4-cycle batch fermentation using IC-TSC significantly improved the ABE productivity. After 48 h of cyclic fermentation, the maximum ABE productivity was 0.43 g/L/h with acetone, butanol and ethanol concentrations of 6.6, 12.9, and 1.1 g/L, respectively.
Theerawat Suratago,Muenduen Phisalaphong,Siriporn Taokaew,Nitisak Kanjanamosit,Kijchai Kanjanaprapakul,Vorakan Burapatana 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.32 No.-
A bacterial cellulose (BC) hydrogel was modified via impregnation by diffusion of sodium alginate andcross-linking with calcium chloride solution. BC/alginate (BCA) membranes were configured,characterized, and used for pervaporation of mixtures of ethanol–water. BCA membranes were morehydrophilic with a more dense structure and showed improved affinity toward water and considerableenhancement of pervaporation performance. At a permeate pressure of 10 mmHg, the BCA membraneimpregnated with 3% (w/v) alginate solution separated water from ethanol in the feed solution (95%, w/w) with 429.9 selectivity, and a total permeate flux of 33 g/(m2 h).
Arnon Khamkeaw,Muenduen Phisalaphong 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.2
Loog-Pang (Thai rice cake starter) is an effective and inexpensive microbial source for the hydrolysis of cassava starch to glucose. A process for hydrolysis of cassava starch to glucose by Loog- Pang was improved by co-immobilized multi-microorganisms (IC) using thin shell silk cocoon (TSC). After incubation at 35oC for 120 h, the IC-TSC system converted 20% w/v cassava starch slurry into clear glucose syrup containing a glucose concentration of 145.5 g/L (composed of 98.8% glucose and 1.2% oligosaccharides), with little or no contamination by microorganisms. The glucose concentration from the starch hydrolysis process using the IC-TSC system was approximately 1.3 times more than that of suspended cultures (SC). The starch hydrolysate could be used as the carbon source for ethanol fermentation without sterilization. A concentration of ethanol of 71.2 g/L (9.1%, v/v) was obtained at 36 h fermentation of the starch hydrolysate by Saccharomyces cerevisiae M30.
Alumina-doped Alginate Gel as a Cell Carrier for Ethanol Production in a Packed-bed Bioreactor
Jirawan Mongkolkajit,Jiranan Pullsirisombat,Savitree Limtong,Muenduen Phisalaphong 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.3
Alumina-doped alginate gel (AEC) was developed as a new type of cell carrier to be used in ethanol fermentation. The presence of the alumina particles in alginate gel not only improved the porous structure of the carrier, but also provided many advantageous characteristics including good mechanical strength, high stability, and high immobilization yield. The attachment of alumina particles and yeast cells by electrostatic attraction was shown to promote cell growth and increase ethanol productivity. The AEC carrier was found to be more effective for the immobilization of Saccharomyces cerevisiae M30 than the conventional Ca-alginate bead. Ethanol productivities of 1.4 and 7.9 ~ 12.6 g/(L/h) were obtained using the AEC cultures in batch and continuous modes of operation, respectively, with an ethanol yield of 43.9 ~ 46.7% and an immobilized yield of 81.4 ~ 84.5%. Ethanol fermentation in a continuous packed-bed reactor using the AEC carrier was stable for > 30 days.
Jeerun Kingkaew,Suchata Kirdponpattara,Neeracha Sanchavanakit,Prasit Pavasant,Muenduen Phisalaphong 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.3
Bacterial cellulose-chitosan (BC-C) films weredeveloped by immersing purified BC pellicles in 1.5 ~2.0% (w/v) acetic acid solutions containing chitosan ofvarying molecular weights. Effects of different molecularweight of chitosan on physical, biological and antimicrobialproperties of the composite films were investigated. Thecumulative chitosan absorption capacities with Mw of141,000, 199,000, and 263,000 were 38.43, 24.65, and23.89 mg/cm3 of dry BC film, respectively. The cumulativerelease profiles of chitosan from the films stronglydepended on molecular weight of chitosan and pH ofsolution. The order of release of chitosan from the BC-Cfilms was dependent on molecular weight as follows: Mw141,000 > Mw 199,000 > Mw 263,000. All BC-C filmsshowed the antimicrobial abilities against Staphylococcusaureus and Aspergillus niger but had no inhibitory effecton the growth of Escherichia coli. The BC-C filmssupported for adhesion, spreading and proliferation of bothhuman skin keratinocytes and fibroblasts. The antibacterialactivity against S. aureus of the BC-C with the highest Mwchitosan (263,000) was higher than those of the others. Onthe other hand, the BC-C films with the lowest Mw chitosan(141,000) promoted the growth of human skin cells morethan those of the others.