Effect of laser-dimpled titanium surfaces on attachment of epithelial-like cells and fibroblasts

Alireza Moshaverinia,Seong-Ho Choi,Jae-Jun Ryu,Sahar Ansari,Meyoung-Kon Kim,Jae-Gu Kim,Dong-Woon Lee 대한치과보철학회 2015 The Journal of Advanced Prosthodontics Vol.7 No.2

PURPOSE The objective of this study was to conduct an in vitro comparative evaluation of polished and laserdimpled titanium (Ti) surfaces to determine whether either surface has an advantage in promoting the attachment of epithelial-like cells and fibroblast to Ti. MATERIALS AND METHODS Forty-eight coin-shaped samples of commercially pure, grade 4 Ti plates were used in this study. These discs were cleaned to a surface roughness (Ra: roughness centerline average) of 180 nm by polishing and were divided into three groups: SM (n=16) had no dimples and served as the control, SM15 (n=16) had 5-µm dimples at 10-µm intervals, and SM30 (n=16) had 5-µm dimples at 25-µm intervals in a 2 × 4 mm2 area at the center of the disc. Human gingival squamous cell carcinoma cells (YD-38) and human lung fibroblasts (MRC-5) were cultured and used in cell proliferation assays, adhesion assays, immunofluorescent staining of adhesion proteins, and morphological analysis by SEM. The data were analyzed statistically to determine the significance of differences. RESULTS The adhesion strength of epithelial cells was higher on Ti surfaces with 5-µm laser dimples than on polished Ti surfaces, while the adhesion of fibroblasts was not significantly changed by laser treatment of implant surfaces. However, epithelial cells and fibroblasts around the laser dimples appeared larger and showed increased expression of adhesion proteins. CONCLUSION These findings demonstrate that laser dimpling may contribute to improving the periimplant soft tissue barrier. This study provided helpful information for developing the transmucosal surface of the abutment.

Salivary Gland Tumors: A Clinicopathologic Study of 366 Cases in Southern Iran

Jaafari-Ashkavandi, Zohreh,Ashraf, Mohammad-Javad,Moshaverinia, Maryam Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1

Background: Salivary gland tumors (SGT) are one of the most complex human neoplasms, demonstrating variations in their clinicopathological profile related to racial and geographic differences. Few studies with large samples have been reported in Iran. We here investigated a large group of patients in southern Iran. Materials and Methods: In this retrospective study, all cases of primary epithelial salivary gland tumors, which had been recorded in a 5 years period from 2005-2009, were enrolled. Clinical data such as histopathologic type and site of the lesion as well as patients' age and gender were analyzed. Results: Data of 366 cases of SGTs were recorded. Pleomorphic adenoma (80.2%) and adenoid cystic carcinoma (46.6%) were the most common benign and malignant neoplasms. Male to female ratio (M/F) and the mean age of patients were 1:1.05 and 37.7 for benign tumors while they were 1.2:1 and 50.6 for malignant tumors, respectively. Parotid and minor salivary glands were involved more frequently. Conclusions: Although the salivary gland tumours encountered were similar in most of their characteristics to those reported in other countries, some differences such as relative frequency and age and gender prevalence were discovered. These findings should help surgeons and pathologists for more accurate diagnosis, management and treatment.

Effect of laser-dimpled titanium surfaces on attachment of epithelial-like cells and fibroblasts

Lee, Dong-Woon,Kim, Jae-Gu,Kim, Meyoung-Kon,Ansari, Sahar,Moshaverinia, Alireza,Choi, Seong-Ho,Ryu, Jae-Jun The Korean Academy of Prosthodonitics 2015 The Journal of Advanced Prosthodontics Vol.7 No.2

PURPOSE. The objective of this study was to conduct an in vitro comparative evaluation of polished and laser-dimpled titanium (Ti) surfaces to determine whether either surface has an advantage in promoting the attachment of epithelial-like cells and fibroblast to Ti. MATERIALS AND METHODS. Forty-eight coin-shaped samples of commercially pure, grade 4 Ti plates were used in this study. These discs were cleaned to a surface roughness (Ra: roughness centerline average) of 180 nm by polishing and were divided into three groups: SM (n=16) had no dimples and served as the control, SM15 (n=16) had $5-{\mu}m$ dimples at $10-{\mu}m$ intervals, and SM30 (n=16) had $5-{\mu}m$ dimples at $25-{\mu}m$ intervals in a $2{\times}4mm^2$ area at the center of the disc. Human gingival squamous cell carcinoma cells (YD-38) and human lung fibroblasts (MRC-5) were cultured and used in cell proliferation assays, adhesion assays, immunofluorescent staining of adhesion proteins, and morphological analysis by SEM. The data were analyzed statistically to determine the significance of differences. RESULTS. The adhesion strength of epithelial cells was higher on Ti surfaces with $5-{\mu}m$ laser dimples than on polished Ti surfaces, while the adhesion of fibroblasts was not significantly changed by laser treatment of implant surfaces. However, epithelial cells and fibroblasts around the laser dimples appeared larger and showed increased expression of adhesion proteins. CONCLUSION. These findings demonstrate that laser dimpling may contribute to improving the peri-implant soft tissue barrier. This study provided helpful information for developing the transmucosal surface of the abutment.

Minced Pulp as Source of Pulpal Mesenchymal Stem Cells with Odontogenic Differentiation Capacity

Liang, Zhangrui,Kawano, Satoshi,Chen, Wei,Sadrkhani, Moein Seyed,Lee, Chaehwan,Kim, Euiseong,Moshaverinia, Alireza,Kim, Reuben H.,Kang, Mo K. Elsevier 2018 JOURNAL OF ENDODONTICS - Vol.44 No.1

<P><B>Abstract</B></P> <P><B>Introduction</B></P> <P>Pulp tissue regeneration is becoming a reality after discovery of mesenchymal stem cells (MSCs) residing in the pulp tissues through various clinical innovations, although MSC transplantation into the pulp space has met with challenges of <I>in vitro</I> cell expansion and cultures. As a way to circumvent the regulatory and technical complexities of <I>in vitro</I> MSC culture, we investigated the use of minced pulp tissues as a source of pulpal MSCs for tissue regeneration.</P> <P><B>Methods</B></P> <P>We characterized the phenotype of cells explanted from minced pulp (MP), namely MP-derived MSCs (MP-MSCs), compared with dental pulp stem cells (DPSCs) established from pulp tissues by enzyme digestion. Phenotypic characterization included replication kinetics, immunophenotyping, and multilineage differentiation. Using the tooth slice model, we assessed odonto/osteogenic differentiation of DPSCs, MP-MSCs, and minced pulp tissues <I>in situ</I>.</P> <P><B>Results</B></P> <P> <I>In vitro</I> replication of MP-MSCs occurred more rapidly during the initial phase of subcultures compared with DPSCs; however, MP-MSCs arrived at senescence at population doubling 47, whereas DPSCs replicated until population doubling 64, indicating shorter replicative lifespan. MP-MSCs also demonstrated stronger odonto/osteogenic differentiation than DPSCs by alkaline phosphatase activity and the protein expression. Both MP-MSCs and DPSCs demonstrated odonto/osteogenic and adipogenic differentiation capacities. Both cell types also showed mineralized tissue formation in the tooth slice model. Seeding minced pulp tissue on poly-L-lactic acid scaffold allowed for migration of MP-MSCs from the tissues and odontogenic differentiation with dentin sialophosphoprotein expression in the tooth slice model.</P> <P><B>Conclusions</B></P> <P>These data indicated that MP may be an alternative source of pulpal MSCs that may allow <I>de novo</I> pulp-dentin regeneration without the need for <I>in vitro</I> culture and expansion.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Revascularization leads to ectopic tissue formation and falls short in <I>bone fide</I> pulp-dentin regeneration. Prior studies show requirement of pulp cell transplantation for pulp tissue regeneration. </LI> <LI> Cells that migrate from the explanted pulp tissues demonstrate distinct replicative and differentiation phenotype from dental pulp stem cell cultures established by enzyme digestion, whereas both cell types exhibit similar surface markers. </LI> <LI> Direct seeding of minced pulp tissue on synthetic scaffold in tooth slice model yielded migrating pulp cells with odontogenic differentiation capacity. </LI> </UL> </P>