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        Termiticidal activity of Acorus calamus Linn. rhizomes and its main constituents against Coptotermes curvignathus Holmgren

        Morina Adfa,Fio Livandri,Neva Putri Meita,Syalfinaf Manaf,Masayuki Ninomiya,Irfan Gustian,Agus Martono Hadi Putranto,Rochmah Supriati,MamoruKoketsu 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.1

        The termiticidal activity of n-hexane and methanol fractions of Acorus calamus Linn rhizomes against Coptotermes curvignathus Holmgren was investigated. Both fractions have demonstrated strong termiticidal activity. The activity of n-hexane fraction was stronger than that of the methanol fraction. Based on GC-MS data, β-asarone was found to be a major constituent of n-hexane fraction (70.1%) and isolation of β-asarone was carried out on column chromatography to afford as a yellow oil in 59.1% yield. Furthermore, the antitermite properties of β-asarone and α-asarone of the isomer were investigated. Our data suggested that the two asarone isomers have insect control potential. These findings lead to the discovery of more environmentally friendly termiticides against C. curvignathus.

      • KCI등재

        Chemical Components, Antitermite and Antifungal Activities of Cinnamomum parthenoxylon Wood Vinegar

        ( Morina Adfa ),( Ari Romayasa ),( Arif Juliari Kusnanda ),( Avidlyandi Avidlyandi ),( Salprima Yudha S ),( Charles Banon ),( Irfan Gustian ) 한국목재공학회 2020 목재공학 Vol.48 No.1

        Termiticidal and fungicidal activities of wood vinegar from Cinnamomum parthenoxylon (CP) stem wood have been evaluated against Coptotermes curvignathus and wood rotting fungi (Schizophyllum commune and Fomitopsis palustris). The utilized CP wood vinegar was produced in the operating temperature range 250-300°C pyrolysis. A no-choice test was applied for evaluating termiticidal activity with 33 active termites and antifungal activity using the agar media assay. The result showed that an increase in the concentrations of CP wood vinegar significantly raised the mortality of termite. CP wood vinegar showed high termiticidal activity, organic acids (acetic acid 42.91%, 3-butenoic acid 6.89%, butanoic acid, 2-propenyl ester 2.26%), and ketones (1-hydroxy-2-propanone 5.14%, 3-methylcyclopentane-1,2-dione 2.34%) might be largely contributed to termiticidal activity in addition to other minor components. Furthermore, CP wood vinegar exhibited significant inhibition of fungal growth. These data showed that CP wood vinegar was more toxic to white-rot fungi (S. commune) than brown-rot (F. palustris). The results suggested that phenolic compounds from lignin degradation were responsible for good antifungal activity.

      • KCI등재

        Anti-Termite Activity of Azadirachta excelsa Seed Kernel and Its Isolated Compound against Coptotermes curvignathus

        Morina Adfa,Khafit WIRADIMAFAN,Ricky Febri PRATAMA,Angga SANJAYA,Deni Agus TRIAWAN,Salprima YUDHA S.,Masayuki NINOMIYA,Mohamad RAFI,Mamoru Koketsu 한국목재공학회 2023 목재공학 Vol.51 No.3

        Azadirachta excelsa, is a plant belonging to the same genus as Indian neem (Azadirachta indica), and its use as a pesticide is reported by few studies. Despite being a different species, it is expected to have the same biopesticide potential as A. indica. Therefore, this study aims to investigate the anti-termite activity of n-hexane and methanol extracts of A. excelsa seed kernel at various concentrations against Coptotermes curvignathus. The methanol extract demonstrated greater termicidal activity than n-hexane at doses test of 2%, 4%, and 8%. It also showed 100% termite mortality on the third day of administering the 8% dose. According to the gas chromatography with mass spectrometry data, the putative main components of the n-hexane extract were hexadecanoic acid, ethyl ester (18.99%), 9,12-octadecadienoic acid (Z,Z)- (16.31%), and 9-octadecenal (16.23%). In contrast, the principal constituents of methanol extract were patchouli alcohol (28.1%), delta-guaiene (15.15%), and alpha-guaiene (11.93%). Furthermore, limonoids profiling of A. excelsa methanol extract was determined using Ultrahigh- performance liquid chromatography coupled with quadrupole-Orbitrap high-resolution mass spectrometry. The number of limonoids identified tentatively was fifteen, such as 6-deacetylnimbin, nimbolidin C, nimbolide, 6-acetylnimbandiol, 6-deacetyl- nimbinene, salannol, 28-deoxonimbolide, gedunin, nimbandiol, epoxyazadiradione, azadirone, 2',3'-dihydrosalannin, marrangin, nimbocinol, and azadirachtin. They were the same as those reported in the seed and leaves of A. indica, but its largest component in A. excelsa was 6-deacetylnimbin. As a result, the presence of these compounds may be responsible for the anti-termite activity of A. excelsa seed kernel extract. Additionally, column chromatography of methanol extract yielded 6-deacetylnimbin, which was found to be antifeedant and termiticide against C. curvignathus.

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