중학생의 동성애적 음란물 접촉정도와 동성애 혐오증 및 고정관념과의 관계

유주연;강문희 서울여자대학교 여성연구소 2004 여성연구논총 Vol.19 No.-

The purpose of the present study was to investigate the relationships among the contact frequency of homosexual lewd materials, homophobia, and prejudice against homosexuals of adolescents. A survey was carried out to 389 middle school students in Gyeonggi province. The brief summary of the survey is as follows. First, among 389 subjects the 73.0% had contacted homosexual lewd materials during the last 1 year, and more boys than girls contacted lewd materials. Most of the subjects had watched the internet sex movies. Movies, TV shows, the papers for adults, and sexual photos were also homosexual lewd materials that many of the subjects had watched. The kinds of materials that middle school students have contacted were different based on sex. Boys contacted the internet sex movies while girls contacted fanfic most. All of them had experience to watch movies and TV shows frequently. Second, the results of regression analysis revealed that the relationship between the contact frequency of homosexual lewd materials and homophobia did not show any statistically significant correlation. However, girls showed statistically significant negative correlation between the contact frequency of homosexual materials and homophobia. Third, the results of regression analysis indicated that the relationship between the contact frequency of homosexual lewd materials and prejudice against homosexuals showed statistically significant positive correlation.

침적 모형에 의한 습성침적 플럭스 수치모의

이화운,문난경,임주연 한국환경과학회 2002 한국환경과학회지 Vol.11 No.12

The purpose of this study is to estimate wet deposition flux and to investigate wet deposition characteristics by using the ADOM model. Wet deposition flux of high reactive SO_2 is estimated by applying observed meteorological parameters and concentrations of chemical species to the ADOM model. Wet deposition is largely dependent on large scale precipitation and cloud thickness. Wet deposition flux of sulfate increases, but wet deposition flux of SO_2 is small. On the whole, the pattern of sulfate flux agrees with the typical pattern of sulfate wet deposition that is high in the summer(July) and low in the winter(January)

범죄 프로파일링에 관한 이론적 고찰

함근수,표주연,강덕지,문완식 內務部 國立科學搜査硏究所 2002 國立 科學 搜査 硏究所 年報 Vol.34 No.-

인지적 법최면 기법에 관한 연구

함근수,표주연,강덕지,문완식 內務部 國立科學搜査硏究所 2001 國立 科學 搜査 硏究所 年報 Vol.33 No.-

급성 진행형 국소 신경학적 증상을 보인 쯔쯔가무시병 1예에서 고용량 스테로이드의 사용

최현철,위성헌,이소연,김상일,박성경,정연주,정소령,전정수,강문원 대한감염학회 2002 감염 Vol.34 No.6

국가직업능력표준 실용화를 위한 제도화 방안

나승일,김주섭,김주일,정연앙,구자길,김강호,문세연 한국농업교육학회 2007 농업교육과 인적자원개발 Vol.39 No.1

이 연구의 목적은 일-교육훈련-자격이 연계될 수 있도록 노동부의 국가직업능력표준을 국가차원에서 개발·운영·관리될 수 있는 제도화 방안을 마련하는데 있었다. 연구목적 달성을 위해 문헌고찰 및 내용분석, 포커스그룹인터뷰를 실시하였고, 연구결과의 타당성 확보를 위해 전문가협의회 및 공청회를 실시하였다. 국가직업능력표준 제도화는 표준의 실용화를 제고할 수 있도록 관련용어 정의, 총괄기구, 표준개발 및 승인, 표준 활용에 관한 인프라 구축에 필요한 사항이 규정되도록 관계 법령을 정비하는 것이다. 표준 제도화를 위해 다음과 같은 사항이 관계 법령에 명시되도록 해야 한다. ① 표준 제도화를 위해 직업, 직업능력, 직무, 표준 등을 관련법에 정의해야 한다. ② 국가직업능력표준 관련 부처 및 다양한 이해당사자의 참여를 기반으로 한 국가직업능력표준 사업 전담 조직을 설치하고, 표준사업에 대한 자문과 심의를 담당할 수 있는 위원회를 구축한다. ③ 다양한 산업분야 단체가 표준개발의 주체가 될 수 있도록 지원하고, 표준의 수준 설정을 통해 직종 내 수직적 경력개발을 촉진하며, 개발된 표준에 대한 지속적인 관리와 현장적합성을 승인하는 체제를 구축해야 한다. ④ 국가직업능력표준은 산업현장에서는 인력을 채용하고 관리하는 기준으로, 교육훈련에서는 교육과정을 개발하고 교과목 및 교재를 개발하며 현 교육과정을 평가하는 기준으로, 자격체계와는 표준의 자격화, 새로운 자격설계, 자격의 출제기준으로 활용될 수 있도록 지원해야 한다. 이를 위해 국가기술자격법, 근로자직업능력개발법, 산업인력공단법 등 관계 법령을 개정하거나 국가직업능력표준에 관한 특별법을 제정할 필요가 있다. 한편, 국가직업능력표준 제도화 단계에 따른 구체적인 추진과제와 이를 실현하기 위한 정책적 제언을 제시하였다. The purpose of this study was to develop the institutionalization that NOS(National Occupational Standard) was developed, operated and managed for linking with work, training and qualification. To achieve the purpose, literature reviews, contents analysis and focus group interview were conducted, and experts council and a public hearing were executed. The institutionalization is the legislation consolidation to regulate details related infra construction as follows for improving utilization of NOS. ① It should be defined occupation, occupational competency, job, standards and so forth on the related laws. ② A organization which take exclusive charge with and a council which consult and deliberate on NOS projects should be established through the cooperation between stakeholder and government organization. ③ It should be supported that various industrial parties including SHRDC are the main body for development of NOS, promoted vertical career development by occupation through establishing level of NOS, and established system that manage continually and approve practical application of NOS. ④ It should be supported that NOS is used as a criterion for recruiting and managing workforce, a criterion for developing and evaluating curriculum and educational materials in training, having qualification for itself, designing for new qualification, and a criterion for setting questions for a qualifying examination. To improve utilization of NOS, It need to be consolidated the related laws including National Technical Qualifications Act and Workers' Vocational Competency Development Act or to be enacted a special law on NOS. Moreover, this study suggested action plan according to the stages of institutionalization, and political proposal for effective actualization of action plan.

2003년 국내 중증급성호흡기증후군 진료 현황 및 문제점 분석

이진수,김은실,정문현,백제중,정선화,안주희,최영화,이선희,고철우,김성범,김민자,박승철,기현균,송재훈,최상호,김양수,이상오,조용균,박영훈,정숙인,김연숙,이흥범,손창희,장성희,정희진,김우주 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

목적 : 2002년 말 중국에서 SARS가 발생한 이후 국내에서도 2003년 10월까지 총 3명의 추정환자, 17명의 의심환자가 보고되었다. 향후 추가적인 SARS의 유행이 우려되는 상황에서, 그간의 SARS 환자 진료에 있어서의 실질적인 준비사항, 진료 현황 등에 대한 조사를 통해 문제점을 파악하여, 향후 더 나은 대비가 될 수 있도록 개선점을 제시하고자 하였다. 재료 및 방법 : SARS로 의심되는 환자를 진료 경험이 있는 병원의료진을 대상으로 2003년 10월에 설문조사를 실시하였다. 설문에는 SARS 환자 진료 시의 실질적인 조치, 진료 현황, 병실, 응급실 및 외래에서의 격리 시설과 준비사항, 보건당국의 관리와 지원에 관한 사항을 포함하였다. 결과 : 대상이 되는 22개 병원 중 17개(17/22, 77.2%) 병원이 설문에 응하였다. SARS 환자를 위한 격리실은 응급실, 외래, 일반병실 및 중환자실에서 각각 9개(9/17, 52.9%), 5개(5/17, 29.4%), 15개(15/16, 93.7%), 4개(4/16, 25%) 병원에서 음압처리가 되어있지 않은 일인실 혹은 다인실이 사용되었고, 1개(1/16, 6.3%) 병원에서만 일반병실에서 음압격리실이 운영되었다. 입원환자의 진찰 시 개인보호구의 착용은 거의 모든 의료기관에서 이루어졌다. 보건당국에서 SARS지정병원의 시설 등을 사전에 확인한 곳은 1곳(1/12, 8.3%)이였고, 14개 병원(14/15, 93.3%)에서는 보건당국에 의뢰한 검사결과를 통하 받지 못하였다. 결론 : 의료기관에서 SARS 환자용 격리실뿐만 아니라 기존의 격리실 설비 등이 미흡하였으며, 특히 중환자실 및 외래의 준비가 더욱 부족하였다. 보건당국의 의료기관에 대한 종합적인 지원이 부족하였고, 병원과의 원활한 연계가 잘 이루어지지 않았다. SARS 만이 아닌 격리를 필요로 하는 질환의 적절한 진료를 위해 향후 병원 시설의 정비와 정부차원에서의 보다 구체적이고 실질적인 대책마련이 필요하다. Background : There was an worldwide outbreak of the Severe Acute Respiratory Syndrome (SARS) originated from China in late 2002. During that period three cases of suspected SARS and 17 cases of probable SARS were reported in Korea. With the concerns about the reemergence of SARS-coV transmission, it is important to be prepared for any possibility. So, this study is aimed to analysis the past measures in managing SARS and propose the amendatory plans to improve the preparedness. Materials & Methods : Questionnaires were collected among clinicians with any experience in managing the probable or suspected SARS cases in Oct. 2003. 17 out of 22 hospitals responded to the questionnaire. The contents in the questionnaire were practical activities, personal equipments, response plans, isolation facilities in emergency centers, outpatient clinics, general wards and intensive care units, and relationship with the public health department. Results : The dedicated isolation rooms in emergency centers, outpatient clinics, general wards, and intensive care units were prepared in 9 (9/17, 52.9%), 5 (5/17, 29.4%), 15 (15/16, 93.7%), and 4 (4/16, 25.0%) hospitals, respectively. Except for one hospital that newly made negative pressure room for SARS, single or multi-bed rooms without airborne infection control were used in all the other hospitals. The personal precaution principles were kept quite well in general wards. Before the designation of SARS hospital by the public health department prior evalution to see if the hospital was suitable for managing SARS was conducted in only 1 (1/12, 8.3%) hospital. The results of laboratory diagnosis were reported back in 1 (1/15, 6.6%) hospital. Conclusions : The isolation facilities which can control airborne infection were almost deficient not only for SARS but also for other respiratory transmissible diseases. For the infection control of transmissible diseases including SARS, more investment is needed on medical facilities and comprehensive support from the public health department required.

Heat-map visualization of gas chromatography-mass spectrometry based quantitative signatures on steroid metabolism

Moon, Ju-Yeon,Jung, Hyun-Jin,Moon, Myeong Hee,Chung, Bong Chul,Choi, Man Ho Springer-Verlag 2009 Journal of the American Society for Mass Spectrome Vol.20 No.9

<P>Abnormalities in steroid hormones are responsible for the development and prevention of endocrine diseases. Due to their biochemical roles in endocrine system, the quantitative evaluation of steroid hormones is needed to elucidate altered expression of steroids. Gas chromatographic-mass spectrometric (GC-MS) profiling of 70 urinary steroids, containing 22 androgens, 18 estrogens, 15 corticoids, 13 progestins, and 2 sterols, were validated and its quantitative data were visualized using hierarchically clustered heat maps to allow “steroid signatures”. The devised method provided a good linearity (<I>r</I><SUP>2</SUP> > 0.994) with the exception of cholesterol (<I>r</I><SUP>2</SUP> = 0.983). Precisions (% CV) and accuracies (% bias) ranged from 0.9% to 11.2% and from 92% to 119%, respectively, for most steroids tested. To evaluate metabolic changes, this method was applied to urine samples obtained from 59 patients with benign prostatic hyperplasia (BPH) versus 41 healthy male subjects. Altered concentrations of urinary steroids found and heat maps produced during this 70-compound study showed also differences between the ratios of steroid precursors and their metabolites (representing enzyme activity). Heat maps showed that oxidoreductases clustered (5<I>β</I>-reductase, 3<I>β</I>-HSD, 3<I>β</I>-HSD, and 17<I>β</I>-HSD, except for 20<I>β</I>-HSD). These results support that data transformation is valid, since 5<I>β</I>-reductase is a marker of BPH and 17<I>β</I>-HSD is positively expressed in prostate cells. Multitargeted profiling analysis of steroids generated quantitative results that help to explain correlations between enzyme activities. The data transformation and visualization described may to be found in the integration with the mining biomarkers of hormone-dependent diseases.</P>

Soybean flower-specific R2R3-MYB transcription factor gene GmMYB108 induces anthocyanin production in Arabidopsis thaliana

Moon Ju Yeon,Lee Saet Buyl,Jeong Yu Jeong,Lim Gah-Hyun,Shin Gilok,Choi Man-Soo,Kim Jeong Ho,Park Ki Hun,Park Ki Hun,Jeong Jae Cheol,Kim Cha Young 한국응용생명화학회 2024 Applied Biological Chemistry (Appl Biol Chem) Vol.67 No.-

R2R3‑MYB transcription factors (TFs) are known to play a key role in regulating the expression of structural genes involved in plant flavonoid biosynthesis. However, the regulatory networks and related genes controlling isoflavo noid biosynthesis in soybean are poorly understood. We previously reported that ethephon application increases the production of isoflavonoids in soybean leaves. In this study, we attempted to identify a potential regulatory gene that positively controls isoflavonoid production in response to ethephon treatment in soybean (Glycine max L.). RNA sequencing (RNA‑seq) revealed that ethephon application led to the upregulation of 22 genes, including the genes for R2R3‑MYB TFs, related to isoflavonoid biosynthesis in soybean plants. Ethephon treatment highly induced the expression of GmMYB108, and its expression was exclusively enriched in flowers as determined using in silico and real‑time quantitative PCR analyses. Furthermore, GmMYB108 overexpression resulted in an intense accumulation of anthocyanins as well as total flavonoid production in the leaf tissues of transgenic Arabidopsis plants. In addition, GmMYB108 overexpression increased the transcript levels of several genes involved in the biosynthesis of anthocya nins and their regulatory pathways in Arabidopsis. These results suggest that GmMYB108 is a potential positive regula tor of the biosynthesis of flavonoids and anthocyanins in soybean flowers.

Simultaneous quantification of 18 saturated and unsaturated fatty acids and 7 sterols as their <i>tert</i>-butyldimethylsilyl derivatives in human saliva using gas chromatography-tandem mass spectrometry

Moon, Ju-Yeon,Kong, Tae Yeon,Jang, Hyun-Jun,Kang, Han Chang,Cho, Yong-Yeon,Lee, Joo Young,Lee, Hye Suk Elsevier 2018 Journal of chromatography. B, Analytical technolog Vol.1092 No.-

<P><B>Abstract</B></P> <P>The profiling of fatty acids (FAs) or sterols has been applied in clinical studies, but still needs to be improved to enable their simultaneous quantification. Moreover, little progress has been made in determining the levels of FAs and sterols in human saliva in a single run. In this study, gas chromatography-tandem mass spectrometry (GC–MS/MS) using one-step <I>tert</I>-butyldimethylsilyl (TBDMS) derivatization was developed for comprehensive profiling of 18 FAs (eight saturated, five monounsaturated, and five polyunsaturated FAs) and 7 sterols (cholesterol and its precursors). The TBDMS derivatization process was also optimized in terms of reaction solvent, catalyst, temperature, and reaction time. The optimized conditions resulted in better derivatization efficiency with good chromatographic separation through a high-temperature column within 23 min. The present method provided good linearity (<I>r</I> > 0.993), precision (coefficient of variation, 2.7% to 10.4%), and accuracy (91.5% to 103.4%). The overall recovery ranged from 73.8% to 114.3% for the 18 FAs, and from 68.9% to 79.8% for the 7 sterols. The validated method was applied to characterize FAs and sterols in human saliva samples. This is the first report of a GC–MS/MS method for the simultaneous determination of various FAs and sterols from a small volume (100 μL) of saliva. This approach can be used as a primary screening tool to examine the levels of both FAs and sterols in saliva, providing detailed information about their homeostasis for diagnostic and prognostic purposes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Both fatty acids and sterols were simultaneously measured from a small volume of human saliva. </LI> <LI> One-step <I>tert</I>-butyldimethylsilylation was developed for the simultaneous quantification of fatty acids and sterols. </LI> <LI> This GC-MS/MS method is a powerful analytical tool to measure salivary fatty acids and sterols in a single run. </LI> </UL> </P>