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Mok Hur,Yurry Um,Jemin Yoo,So Hyun Park,Yi Lee,Jin Tae Jung,Yeon Bok Kim,Seong Cheol Kim,Chan Sik Jung 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.05
Background : Astragalus membranaceus is one of the most widely used traditional medicinal herbs in Korea. Studies on the genomic of A. membranaceus resources have not been carried out so far. The present study was carried out to discriminate A. membranaceus based on genetic diversity using genomic simple sequence repeat (SSR) markers. Methods and Results : We collected 5 A. membranaceus lines: Asung, Poongsung, Am-Jecheon, Am-Sancheong, and Am-China. One hundred mg of fresh leaves were used for genomic DNA extraction using the DNeasy plant DNA isolation kit (Qiagen GmbH, Hilden, Germany). 450,449 contigs were searched for 147,766 SSR candidate loci in this study using the MicroSAtellite identification tool (MISA). We selected 949 A. membranaceus genomic SSR markers that were showed variation for the five collections in silico screening with CLC genomics workbench program. The genetic diversity of all A. membranaceus resources was analyzed using 17 SSR markers employing the DNA fragment analysis method. Based on the genetic diversity analysis, these lines were classified into four distinct groups. Conclusion : These findings could be used for further research on cultivar development using molecular breeding techniques and for conservation of the genetic diversity of A. membranaceus. Furthermore, the markers could be used for marker-assisted selection for crop breeding.
Mok Hur,Yurry Um,Yeong Bok Kim,Chan Sik Jung,Chan Sik Jung,Seong Cheol Kim 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.10
Background : Platycodon grandiflorum has been used as food material and a traditional medicine in Korea. In order to develop an efficient in vitro micropropagation technique for a rare plant species and conservation for inbred line of plant breeding. Methods and Results : Plant regeneration via organogenesis and somatic embryogenesis was investigated in Platycodon grandiflorum. Leaf, stem, root tissues of 7-day-old seedlings were cultured on 1/2MS medium containing various concentration (0, 0.5, 1 and 2 ㎎/L) of IBA, BA and NAA. The results showed that 1/2MS medium supplemented with BA+NAA 2.0 ㎎/L yielded the highest callus formation ratio of 73.5%. When various tissues (leaf, stem, root) were tested on 1/2MS medium containing BA 2.0 ㎎/L+ NAA 2.0 ㎎/L for somatic embryogenesis, the optimum tissue for embryogenic shoot induction was stem tissue. Callus were cultured on MS medium containing various concentration (0, 0.5 and 1 ㎎/L) of BA and NAA. The best rooting rate was achieved by three different medium (B5, 1/2MS and MS) and 1/2MS medium cultured the highest rooting ratio (82%). Conclusion : This study suggested that above micropropagation techniques can be used for rapid multiplication as well as in vitro or in vivo conservation of the Platycodon species.
A Study on the Stability of MgO in a LiCl-Li₂O Molten Salt System
Hur, Jin-Mok,Kim, Ik-Soo,Lee, Won-Kyoung,Cho, Soo-Haeng,Seo, Chung-Seok,Park, Seong-Won 한국공업화학회 2004 Journal of Industrial and Engineering Chemistry Vol.10 No.3
The stability of MgO was studied to verify its applicability to the LiCl-Li₂O molten salt based process. The interaction of MgO not only with molten salt but also with CO₂ and H₂O in relation to its property as a solid base was investigated. MgO was chemically stable at the typical operation temperature of the LiC1-Li₂O molten salt based process, 650℃. However, the stabilization of L i 2 0 structure on a MgO surface was observed when MgO is withdrawn from the molten salt. The result was clearly characterized by means of thermo-gravimmetry analysis, differential thermal analysis, X-ray diffraction analysis, thermodynamic analysis, and a CO₂ temperature programmed desorption. MgO can be considered as an acceptable candidate material for the high temperature molten salt industry.