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Potential suitable habitat of Buckwheat (Fagopyrum spp) under the climate change scenarios in Nepal
Dol Raj Luitel,Siwakoti Mohan,Mohan D. Joshi,Rangaswami Muniappan,Pramod K. Jha 한국작물학회 2021 Journal of crop science and biotechnology Vol.24 No.4
Buckwheat is minor crop of Nepal. Due to their multiple uses, short growing periods, high nutritional and medicinal values, wide adaptability has become popular not only to researcher but also for farmers and consumers globally. It grows in a wider range from low land Terai to higher mountains of Nepal. Maxent modelling was used to quantify the current suitable habitat and predicted future suitable area under diferent climate change scenarios, based on representative concentration pathways (RCP 4.5 and 8.5) in two diferent time periods (2050 and 2070 AD) using climatic predictive variables and species localities. The most suitable habitat area of cultivation, area loss, and gained for buckwheat were determined. The model showed that about 46% area of country is climatically suitable for buckwheat within the elevation range of 289–4441 m. Habitat suitability of buckwheat would shrink by 7.5% and 8.2% under RCPs 4.5 and by 7.6% and 8.3% under 8.5 RCPs in the year 2050 and 2070, respectively. The loss and gain area analysis also indicated that suitable area would be lost more than gained in both 2050 and 2070. These fndings are expected to support planning and policy framing for climatic resilience smart agriculture practice to meet the livelihood and food security problems in the mountains of Nepal. Due to medicinal value of buckwheat, its demands is increasing. The increasing demend of buckwheat would be fulflled by increasing production under climatically suitable area under climate change scenarios.
Subramanian Mohan Raj,Chelladurai Rathnasingh,Woo-Chel Jung,Edwardraja Selvakumar,박성훈 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.1
3-Hydroxypropionic acid (3-HP), a versatile and valuable platform chemical, has diverse industrial applications; but its biological production from glycerol is often limited by the capability of the enzyme aldehyde dehydrogenase (ALDH) to convert an intermediary compound, 3-hydroxypropionaldehyde (3-HPA), to 3-HP. In this study, we report a new ALDH, PuuC, from Klebsiella pneumoniae DSM 2026, that efficiently converts 3-HPA to 3-HP. The identified gene puuC was cloned, expressed in Escherichia coli, purified, and characterized for its properties. The recombinant enzyme with a molecular weight of 53.8 kDa exhibited broad substrate specificity for various aliphatic aldehydes, especially C2-C5 aldehydes. NAD+ was the preferred coenzyme for the oxidation of most aliphatic and aromatic aldehydes tested. The optimum pH and temperature for PuuC activity were pH 8.0 and 45oC. The Km values for 3-HPA and NAD+ were 0.48 and 0.09 mM, respectively. The activity of PuuC was enhanced in the presence of reducing agents such as 2-mercaptoethanol or dithiothreitol, while several metal ions, particularly Hg2+, Ag+, and Cu2+ inhibited its activity. The predicted structure of PuuC indicated the presence of K191 and E194 in close proximity to the glycine motif, suggesting that PuuC belongs to class 2 ALDHs.
Raj, Kathamuthu Gokul,Sambantham, Shanmugam,Manikanadan, Ramar,Arulvasu, Chinnansamy,Pandi, Mohan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.16
Purpose: The present study concerns molecular mechanisms involved in induction of apoptosis by a fungal taxol extracted from the fungus Cladosporium oxysporum in T47D human breast cancer cells. Materials and Methods: Apoptosis-induced by the fungal taxol was assessed by MTT assay, nuclear staining, DNA fragmentation, flow cytometry and pro- as well as anti-apoptotic protein expression by Western blotting. Results: Our results showed inhibition of T47D cell proliferation with an $IC_{50}$ value of $2.5{\mu}M/ml$ after 24 h incubation. It was suggested that the extract may exert its anti-proliferative effect on human breast cancer cell line by suppressing growth, arresting through the cell cycle, increase in DNA fragmentation as well as down-regulation of the expression of NF-${\kappa}B$, Bcl-2 and Bcl-XL and up-regulation of pro-apoptotic proteins like Bax, cyt-C and caspase-3. Conclusions: We propose that the fungal taxol contributes to growth inhibition in the human breast cancer cell through apoptosis induction via a mitochondrial mediated pathway, with possible potential as an anticancer therapeutic agent.
Rathnasingh, Chelladurai,Raj, Subramanian Mohan,Jo, Ji-Eun,Park, Sunghoon Wiley Subscription Services, Inc., A Wiley Company 2009 Biotechnology and bioengineering Vol.104 No.4
<P>3-Hydroxypropionic acid (3-HP) is a commercially valuable chemical with the potential to be a key building block for deriving many industrially important chemicals. However, its biological production has not been well documented. Our previous study demonstrated the feasibility of producing 3-HP from glycerol using the recombinant Escherichia coli SH254 expressing glycerol dehydratase (DhaB) and aldehyde dehydrogenase (AldH), and reported that an “imbalance between the two enzymes” and the “instability of the first enzyme DhaB” were the major factors limiting 3-HP production. In this study, the efficiency of the recombinant strain(s) was improved by expressing DhaB and AldH in two compatible isopropyl-thio-β-galactoside (IPTG) inducible plasmids along with glycerol dehydratase reactivase (GDR). The expression levels of the two proteins were measured. It was found that the changes in protein expression were associated with their enzymatic activity and balance. While cloning an alternate aldehyde dehydrogenase (ALDH), α-ketoglutaric semialdehyde dehydrogenase (KGSADH), instead of AldH, the recombinant E. coli SH-BGK1 showed the highest level of 3-HP production (2.8 g/L) under shake-flask conditions. When an aerobic fed-batch process was carried out under bioreactor conditions at pH 7.0, the recombinant SH-BGK1 produced 38.7 g 3-HP/L with an average yield of 35%. This article reports the highest level of 3-HP production from glycerol thus far. Biotechnol. Bioeng. 2009; 104: 729–739 © 2009 Wiley Periodicals, Inc.</P>