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      저자 : Mohammad Reza Nasri Nasrabadi (검색결과 2 건)
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        High Levels Lycopene Accumulation by Dietzia natronolimnaea HS-1 Using Lycopene Cyclase Inhibitors in a Fed-batch Process

        Mohammad Reza Nasri Nasrabadi,Seyed Hadi Razavi 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.4

        In an attempt to accumulate lycopene in the bacterium Dietzia natronolimnaea HS-1 cultured in beet molasses, the influence of lycopene cyclase inhibitors on carotenogenesis (within the range 0-60 ppm) were evaluated. All inhibitors blocked the biosynthesis of canthaxanthin and resulted in accumulation of lycopene as the predominant carotenoid. As the final step of a study aiming at the optimization of culture conditions, a central composite design to a fed-batch process was applied to optimize the concentrations of lycopene cyclase inhibitors (within the range 0-50 ppm) in order to achieve high level accumulation of lycopene from D. natronolimnaea HS-1cultured in beet molasses. On the basis of this approach,the optimum concentrations of lycopene cyclase inhibitors required to achieve the highest level of lycopene accumulation (8.26±0.17 mg/L) were determined as follows: imidazole,24.74 ppm; nicotinic acid, 28 ppm; piperidine, 24.05 ppm;pyridine, 27.6 ppm; and triethylamine, 23.22 ppm.

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        Optimization of β-Carotene Production by a Mutant of the Lactosepositive Yeast Rhodotorula acheniorum from Whey Ultrafiltrate

        Mohammad Reza Nasri Nasrabadi,Seyed Hadi Razavi 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.2

        The present work investigated on carotenogenesis with high β-carotene content by a new isolated highactivity strain-producer Rhodotorula acheniorum mutant MRN in cheese whey ultrafiltrate. After a serial of UV,ethymethanesurfonate (EMS), and nitrosoguanidine (NTG)mutagenesis, a mutant named MRN of the red lactosepositive yeast strain R. acheniorum was obtained. Then, the effects of different growth medium factors on carotenoid production by this mutant at batch-scale level were identified and optimized by means of response surface methodology (RSM) in order to achieve high-level production of β-carotene. The optimum conditions required to achieve the highest level of β-carotene (262.12±1.01mg/L) were determined as follows: whey ultrafiltrate (WU)lactose concentration 55 g/L, pH 5.85, ammonium sulfate concentration 3.5 g/L, temperature 23^oC, and aeration rate 1.56 vvm. The medium optimization resulted in a 6.45-fold increase in volumetric production (262.12±1.01 mg/L) and a 4.62-fold increase in the cellular accumulation (10.69±0.19 mg/g) of β-carotene.

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