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RISS 인기검색어
- 검색키워드
- 저자 : Ming-Lei Zhuang (검색결과 2 건)
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Ming-Lei Zhuang,Shen-Min Song 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.7
This paper studies the fixed-time stability of coordinated attitude tracking control under consideration of an input amplitude constraint. The attitude dynamics is described by rotation matrix, which avoids the unwinding phenomenon. By designing and analyzing the proposed nonsingular terminal sliding mode surfaces (NTSMSs) step by step, a novel saturated NTSMS is finally established. This NTSMS has theoretical and practical advantages over other existing NTSMSs, such as its fixed-time convergence property and boundedness. Based on it, two fixed-time coordinated controllers are developed. The first one effectively decreases the amplitude of the control input, whereas the second prevents saturation. Both controllers keep the attitude synchronization task be finished in fixed-time and provide better performance than existing controllers. Specific expressions of the regions to which the system states to converge are obtained using Lyapunov stability theory. Simulation results testify the correctness of the proposed theorems.
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Knockout of the EgriBLOS2 gene results in the transparent integuments of Ectropis grisescens larvae
Li Jia-Li,Zhuang Xiang-Lin,Yuan Ting-Ting,Cai Xiao-Ming,Luo Zong-Xiu,Bian Lei,Chen Zong-Mao,Li Zhao-Qun,Liu Nai-Yong 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.1
The body colors of insects have evolved specialized roles in body protection, signaling and physiology. In some case, the larvae can camouflage their body colors to adapt the habitants and protect themselves. However, the genetic and molecular basis on larval body colors of the tea geometrid, Ectropis grisescens, remains poorly known. Here, we reported an effect of the lysosome-related organelles complex-1, subunit 2 (EgriBLOS2) gene knockout on larval integuments of E. grisescens, by using a clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system. Combining transcriptomic data and PCR approaches, we first identified the EgriBLOS2 gene from E. grisescens, which encoded 137 amino acids and comprised 3 introns. In the phylogenetic analysis, EgriBLOS2 clustered in the Lepidoptera clade with high conservation to members in other lepidopteran species. Developmental expression profiles revealed that EgriBLOS2 was constantly tran scribed at each stage, in which its expression was significantly lower in 2nd instar larvae than that of other instars. After injecting EgriBLOS2-specific guide RNA and Cas9 messenger RNA into eggs, 70% of larvae showed the translucent integuments in G0 generation, with an emphasis on black splayed patterns in the 2nd and 8th segments of abdomens. However, some typical characteristics of larvae were not obviously changed in mutant instars, such as ocelli, mouthparts and other appendants. This study has unraveled the roles of EgriBLOS2 in the formation of larval integument colors, and provides an alternative strategy for pest management based on the colors in this species.
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