염전법이 병행된 침자극 및 전침자극이 진통효과에 미치는 영향

朴東錫,閔炳一,盧植,金志勳 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 1998 東西醫學硏究所 論文集 Vol.1998 No.-

Twirling the needle (TN) is one of several needling methods frequently used for acupuncture in the oriental medicine. The present study was conducted to see if TN enhanced the antinociceptive effect produced by plainor electroacupuncture in the rat tail flick test. Rats (Sprague-Dawley, 200~300g) lightly anesthetized with thiopental sodium (40mg/kg, i.p.) were used in the tail-flick test. The basal reaction time for tail-flick was 2 sec. For "plain" acupuncture (PA), a needle was inserted for 20 min into a Chock-samni(ST36) acupuncture point which (is located at the anterior tibial muscle and about 10mm below the knee joint). For electroacupuncture (EA), train-pulses (3Hz, 0.3ms pulse width, 0.2!0.3mA) were applied to the inserted needle for the period of insertion. For combining TN with either PA or EA, TN was performed during the each acupuncture period. Two methods for TN were used; twirling 3 times for 1 second persisted for 1 min in every 5 mins (long-duration and long-interval; LDLI)and twirling 3 times for 1 second persisted for 10 sec in every 1 min (short-duration and short-interval; SDSI). PA and EA produced an increase in tail flick latency (TFL); peak increases were 21.3??5.1% and 42.1??17.3% of the pre-acupunture control, respectively, and occurred immediately after cessation of 20 min-acupuncture. Performing TN combined with PA increased TFL more than PA itself by showing a greater peak increase in TFL when performed with a SDSI-TN than with a LDLI-TN (60.6??12.5% and 42.7??22.7% of the pre-acupuncture control, respectively). Performing TN combined with EA also increased TFL more than EA itself and a greater peak increase in TFL was observed with a SDSI-TN as compared to a LDLI-TN (77.5??13.8% and 67.3??14.0% of the pre-acupuncture control, respectively). These results indicate that TN enhances both PA-and EA-produced antinociception where an enhanced antinociception than a LDLI-TN. It is suggested that performing a SDSI-TN combined with EA seems to be one of the most effective ways to produce analgesia.

捻轉法이 竝行된 鍼刺戟 및 電鍼刺戟이 鎭痛效果에 미치는 影響

金志勳,盧植,閔炳一,朴東錫 경희대학교 1998 慶熙醫學 Vol.14 No.3-4

건설공사의 안전투자비에 대한 효과 분석

박종근,노민래,이관형 한국안전학회 2004 한국안전학회지 Vol.19 No.4

This study delivers the actual condition of investment for industrial accident prevention based on survey of 500 construction sites from "reports for industry safety and health" published by Korea Occupational Safety & Health Agency (KOSHA). The various research techniques were used such as technical statistic analysis for construction industry, cost comparison of industrial accident prevention and accident loss. A formula was deduced to calculate accident loss and accident frequency by accident prevention cost through regression analysis.

토양환경지도 자료를 이용한 콩밭 토양의 화학성 비교

박지숙,이민진,정재원,김미혜,이서연,지윤미,한준호,김유학,공명석,한광현,노희명 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

It is important to interpret the soil chemical properties in order to effectively manage the farmland. This study was conducted to investigate the soil chemical properties of upland soybean field according to the soil series at 32 upland fields located in the parts of Gangwon-do. Soil sampling sites were selected by using the National Statistics DB and KSIS DB that is a soil environment information system provided by the Rural Development Administration. Soil samples were collected from 0~15 cm of top soil before seeding or transplanting, air-dried, passed through a 2 mm sieve, and analyzed for soil pH, EC, organic matter, available phosphorus (P2O5), exchangeable potassium, exchangeable calcium and exchangeable magnesium. The average chemical properties have exceeded the each nutrient optimum range level of RDA, except the organic matter in experimental soil 2015. And despite application of conventional fertilization, each soil series nutrient contents were change respectively. The results of this study suggest that conventional fertilization should be applied based on the analysis about the amount of accumulated nutrient contents in the soil.

Clopidogrel에 의해 발생된 전신 염증 반응 증후군 1례

김민환,김종훈,박경일,박혜연,황철웅,김의석,도준형,남궁준,이성윤,이원로 白中央醫療院 2005 仁濟醫學 Vol.26 No.1

Clopidogrel bisulfate, a widely used inhibitor of platelet aggregation, is considered at least as safe as aspirin. We describe a 61 year old male patient who developed a systemic inflammatory response syndrome consisting of high fever, rash, chills, impaired liver function, and mild leukopenia after receiving clopidogrel after coronary angiography and stent implantation. The reaction resolved promptly after withdrawal of the drug, thus making the diagnosis of a clopidogrel induced reaction highly probable.

유색 보리 종실의 품종별 색소 특성

주완택,민경수,박노동 한국응용생명화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.4

유색보리 4품종과 보통보리 4품종 종실의 종피에 함유되어 있는 색소의 특성을 분석하여 해당작물의 신품종 육성시 우수계통 선발에 유용한 자료를 제공코자 하였다. 유색(검정)쌀보리인 Ab2231을 표준시료로 하여 실험한 결과 에탄올의 농도가 80%일 때 색소추출 효율이 양호하였다. 80% 에탄올에 1.0% 염산을 첨가한 것이 추출효율이 가장 좋았다. 추출시간에 따른 추출효율성을 조사한 결과 1시간 추출이면 충분하였다. 추출된 색소의 안정성을 유지하기 위하여 첨가시키는 산의 적정농도를 알아본 결과 1.0% HCl을 첨가한 농도에서 가장 낮은 색소량 감소율이 나타났다. 각 보리 품종 시료에서 추출한 색소의 흡광스펙트럼을 조사하였던 바, Ab2231의 그것은 535㎚에서 최대흡광파장을 나타내어 안토시아닌계 색소로 판단되었으며, 호계4호의 그것은 안토시아닌계 이외의 색소를 주로 함유하는 것으로 보였다. 한편, 추출된 색소의 특성에 따라 유색보리의 품종은 Ab2231와 모찌무기로 대표되는 쌀보리 그룹과 Lion과 호계4호로 대표되는 겉보리 그룹으로 분류되었다. The characteristics of the pigments extracted from grains of the colored barley varieties Ab2231. Mozzimugi, Lion, and Hogye 4 were studied. Extraction of the pigments from powder (80 mesh) of the barley grains was most efficient in 80% ethanol containing 1.0% HCI for 1 hr at 60℃. The pigments were also stable in 80% ethanol containing 1.0% HCI. Depending on the absorption spectra, maximal peak wavelength, and the ratio of A_(440)/Anu_(max), the varieties were separated into two groups, colored naked barley (Ab2231 and Mozzimugi) and colored covered barley (Lion and Hogye 4).

急性骨髓性 白血病에서의 試驗管內 集落細胞 形成에 관한 연구

고석만,조덕연,박철산,강원권,김민범,김삼용,노홍규 忠南大學校 癌共同硏究所 1991 癌共同硏究所 硏究誌 Vol.1991 No.-

急性骨髓性 白血病에서의 試驗管內 集落細胞 形成에 관한 연구

고석만,조덕연,박철신,강원권,김민범,김삼용,노흥규 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.2

To evaluate the in vitro granulocyte-macrophage colony formation in acute myfloblastic leukemia and the prognostic implications of these results, the author performed the in vitro agar culture of bone marrow cells in 10 patients with acute myeloblastic leukemia (AML) and 5 control subjects. Culture medium was composed of 20% fetal calf serum(FCS), 50% Iscove's medium, 0.3% agar, 10% colony stimulation factor (CSF), and 2 × 10 exp (5) cells/ml. Human placental conditioned medium (HPCM) and phytohemagglutinin-leukocyte conditioned medium (PHA-LCM) were used as colony stimulating factor. Colony counting was done on 7th day of culture. Colony was defi ned as containg 20 or more cells, and cluster was defined as containing 3-19 cells. The results were as followings, 1. In control subjects, the number of clusters formed was 3-47/2 × 10 exp (5) cells (20±19)and that of colonies was 5-24/2 × 10 exp (5) cells (14±9) when stimulated with HPCM. When stimulated with PHA-LCM, the number of clusters formed 5-39/2 × 10 exp (5) cells(18±16) and that of colonies was 6-13/2 × 10 exp (5) cells (9±3). 2. In AML patients, 3 groups were recognized according to pattern of colony formation: 1) non-forming 2) cluster forming 3) both cluster and colony forming. Of 10 cases, 5 cases Were `non-forming', 2 cases were `cluster forming'; and 3cases wer cluster and `colony forming'; 3. All 5 cases 'non-forming' cases, one of 2 cases of `cluster forming'; and none of 3 cases of `cluster and colony forming' achieved complete remission. So, there was significa nt difference in remission rate in the different growth types. These results suggest that granulopoiesis in AML patients is impaired and the pattern of in vitro CFU-L(colony forming unit-leukemia) formation has prognostic significance.

急性骨髓性 白血症에서의 試驗管內 集落細胞 形成에 관한 연구

고석만,조덕연,박철신,강원권,김민범,김삼용,노흥규 忠南大學校 癌共同硏究所 1991 癌共同硏究所 硏究誌 Vol.1 No.1

To evaluate the in vitro granulocyte-macrophage colony formation in acute myeloblastic leukemia and the prognostic implications of these results, the author performed the in vitro agar culture of bone marrow cells in 10 patients with acute myeloblastic leukemia (AML) and 5 control subjects. Culture medium was composed of 20% fetal calf serum(FCS), 50% Iscove's medium, 0.3% agar, 10% colony stimulation factor (CSF), and 2×105 cells/ml. Human placental conditioned medium (HPCM) and phytohemagglutinin-leukocyte conditioned medium (PHA-LCM) were used as colony stimulating factor. Colony counting was done on 7th day of culture. Colony was defined as containg 20 or more cells, and cluster was defined as containing 3 - 19 cells. The results were as followings, 1. In control subjects, the number of clusters formed was 3-47/2×10^(5) cells (20±19)and that of colonies was 5-24/2×10^(5) cells (14±9) when stimulated with HPCM. When stimulated with PHA-LCM, the number of clusters formed 5-39/2×10^(5) cells(18±16) and that of colonies was 6-13/2×10^(5) cells (9±3). 2. In AML patients, 3 groups were recognized according to pattern of colony formation : 1) non-forming 2) cluster forming 3) both cluster and colony forming. Of 10 cases, 5 cases were 'non-forming', 2 cases were 'cluster forming'; and 3cases wer cluster and 'colony forming'; 3. All 5 cases 'non-forming' cases, one of 2 cases of 'cluster forming'; and none of 3 cases of 'cluster and colony forming' achieved complete remission. So, there was significant difference in remission rate in the different growth types. These results suggest that granulopoiesis in AML patients is impaired and the pattern of in vitro CFU-L(colony forming unit-leukemia) formation has prognostic significance.

Thyrotropin은 STAT1(Signal Transducers and Activation of Transcription 1) 의 활성을 저해하여 Interferon-r에 의한 유전자 발현을 억제한다

이강욱,김영건,노흥규,송민호,김호,박은신,유순희,한희정,주원찬,원진호,임규,권오유 대한내분비학회 1998 Endocrinology and metabolism Vol.13 No.4

Background: The proinflammatory cytokine, IFN-y has been shown to exert pleiotropic effects in a variety of pathophysiologic conditions in autoimmune thyroid disease. The thyrocyte response to IFN-y is mediated two distinct classes of proteins, Janus kinases(Jakl and Jak2) and Signal Transducers and Activation of Transcription(STATl). The activation of STAT 1 is involved in the regulation of many interferon stimulated genes, such as MHC class II, intercellular adhesion molecules-1(ICAM-1) and MHC class II transactivator(CIITA) after the binding to the GASgFN- pactivated site) of the gene promoters. Recently we found TSH/forskolin inhibits IFN-y stimulated maximal expression of ICAM-1 in FRTL-5 cell. IFN-y action is localized between -175 bp and -97 bp from the start of translation of ICAM-1 gene which contains regulatory elements known to be involved in IFN-y action in other eukaryotic cells, palindromic IFN-y activated site(GAS)(5-TTTCCGGGAAA-3) which could bind STAT1, STAT3, STAT5, STAT6. Furthermore, the addition of TSH and forskolin causes a decrease in ICAM-1 promoter activity and its action was localized in GAS. These findings suggested TSH/cAMP signaling pathways downregulate IFN-y activated Janus kinase-STAT signaling path. We wanted to explore the possible involvement of elevated cAMP in the negative regulation of IFN-y induced STAT1 activation in thyroid cells. Method: We made several 5-deletion constructs of rat ICAM-1 promoter and analyzed the promoter activities by measuring the luciferase activity after tranfection into FRTL-5 cells. The protein/DNA complex was measured by electrophoretic mobility shift analysis using labeled oligonucleotide. We checked the level of total and phosphorylated STATl protein by immunoblot analysis using specific antibodies. Results: Stimulation of IFN-y in FRTL-5 cells resulted in rapid activation of STATl/DNA binding activity, which was apparent after several minute of stimulation, maintains its activity until 48 h. Incubation of cells with TSH result in suppression of IFN-p mediated STAT1/DNA binding activity throughout the time course of activation by IFN-y. Addition of TSH into 5H maintained FRTL-5 cells did not change the total amount of latent STAT1 amount and also not affect IFN-y mediated production of total STAT1 until 4 h. IFN-y(100 U/mL) rapidly induced phosphorylation of STAT1 within 30 min. and maintained its level without significant change until 48 hours. Cells treated with TSH dramatically lowered the level of IFN-y induced production and phosphorylation of STAT1 after 12 h, 24 h, 36 h, and 48 h but TSH had no effect on the level of phosphorylated STATl within 4 h after IFN-y stimulation. The proteasome inhibitor, MG132 and phosphatase inhibitor, sodium orthovanadate did not block the TSH or forskolin mediated downregulation of phosphorylated STAT1. Conclusion: These results indicate a regulatory mechanism which TSH signaling can modulate the prolonged activation of Jak/Stat by IFN-y. We identified one of mechanisms related to TSH mediated negative suppression of the ICAM-1 gene; TSH/cAMP signaling pathways downregulate the cytokine activated Janus kinase-STAT signaling path (J Kor Soc Endocrinol 13:536-553, 1998).