부산지역 유흥업소 종사여성으로부터 분리된 HPV16형의 발암유전자(E6/E7) 돌연변이 유형 분석

민상기(Sang-Kee Min),김성순(Sung Soon Kim),최병선(Byeong-Sun Choi),장대호(Dai-Ho Jang),이미옥(Mee-Ok Lee),최성화(Seung-Hwa Choi),김남호(Nam-Ho Kim),박연경(Yon-Koung Park),정영아(Yeong-A Jeong),김성준(Seong-Joon Kim),빈재훈(Jae-Hun Bi 한국생명과학회 2009 생명과학회지 Vol.19 No.6

HPV-16형의 염기배열 변이는 지역적, 인종적으로 특징적인 차이가 있으며 특히 HPV-16형 E6/E7 유전자의 특정 염기서열변이는 자궁경부암 및 자궁상피내 신생종양물의 발생을 일으키는 고위험 요인으로 알려져 있다. 본 연구는 2007년 부산지역 유흥업소 종사여성으로 분리된 HPV-16형 19건을 대상으로 E6/E7 유전자 영역(nt 34-880)을 표적으로 지역적 염기서열 변이를 조사하였다. nucleotide 수준에서 HPV16형 E6 유전자는 T178G (n=11), T178A (n=1), T350G (n=4), A442C (n=2), A104T, A111G, C116T, G145T, T183G, C335T, G522C 등 11종의 변이주가 발견되었고, E7 유전자는 A647G (n=12), A645C, A777C, G663A, T732C, T760C, A775T, T789C, T795G 등 9종의 변이주가 발견되었다. 아미노산 수준에서는 HPV-16형 E6 단백질의 경우 D25E (n=12), L83V (n=4), E113D (n=2), M1L, Q3R, P5S, Q14H, D25N, I27R, H78Y, C140S 등 11종의 변이주를, HPV16형 E7 단백질의 경우 N29S (n=12), L28F, T72S 등 3종의 변이주를 관찰할 수 있었다. 본 연구 결과, 부산지역의 HPV-16형 E6/E7 우점 돌연변이주는 E6 D25E (75%), E7 N29S (78%)로 각각 나타났다. 앞으로 자궁경부암 환자 및 일반여성을 포함한 더 많은 모집단을 대상으로 HPV-16형 E6/E7의 intratypic variants를 비교 조사하여 실제 HPV-16형 E6/E7 어떤 변이주가 자궁경부암 유발 위험성과의 관련성은 더 많이 연구되어져야 할 것으로 사료된다. Recent studies have reported that the distribution of HPV-16 sequence variation differs geographically, and more specifically that HPV-16 E6/E7 intratypic variants might carry a high risk for development of ICC (invasive cervical cancer) and CIN (cervical intraepithelial neoplasia) in a given population. To investigate the genetic diversities of HPV-16 E6/E7 oncogene by region, we collected nineteen HPV-16 isolates from sexually high-risk women in Busan, and analyzed the HPV-16 E6/E7 coding regions (nt 34 to 880) with HPV-16 E6/E7 specific PCR amplification. At the nucleotide level, eleven variants of the E6 genes and nine variants of the E7 genes were identified as follows: E6 T178G (n=11), E6 T178A (n=1), E6 T350G (n=3), E6 A442C (n=2), E6 A104T, E6 A111G, E6 C116T, E6 G145T, E6 T183G, E6 C335T, E6 G522C and E7 A647G (n=12), E7 A645C, E7 A777C, E7 G663A, E7 T732C, E7 T760C, E7 A775T, E7 T789C and E7 T795G, respectively. At the amino acid level, the isolated HPV-16 E6 and E7 genes showed eleven E6 variants: E6 D25E (n=12), E6 L83V (n=4), E6 E113D (n=2), E6 M1L, E6 Q3R, E6 P5S, E6 Q14H, E6 D25N, E6 I27R, E6 H78Y, E6 C140S and three E7 variants: N29S (n=12), L28F, T72S. HPV16 E6 L83V, the dominant variant in the Caucasian population, showed relatively low frequencies in our study population. We elucidated that the dominant HPV-16 E6/E7 variants were HPV-16 E6 D25E (63.2%) and HPV-16 E7 N29S (63.2%), which were phylogenetically included in Asian lineage. Further study is needed to evaluate the risk of cervical cancer related HPV-16 E6/E7 intratypic variants in the Korean population.

Poly(vinylbenzyl chloride-glycidyl methacrylate)/Polyethylene Composite Anion Exchange Membranes for Vanadium Redox Battery Application

Park, Min-A,Shim, Joonmok,Park, Se-Kook,Jeon, Jae-Deok,Jin, Chang-Soo,Lee, Ki Bong,Shin, Kyoung-Hee Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.6

Anion exchange membranes for a vanadium redox flow battery (VRB) were prepared by pore-filling on a PE substrate with the copolymerization of vinylbenzyl chloride (VBC) and glycidyl methacrylate (GMA). The ion exchange capacity, water uptake and weight gain ratio were increased with a similar tendency up to 65% of GMA content, indicating that the monomer improved the pore-filling degree and membrane properties. The vanadium ion permeability and open-circuit voltage were also investigated. The permeability of the VG65 membrane was only $1.23{\times}10^{-7}\;cm^2\;min^{-1}$ compared to $17.9{\times}10^{-7}\;cm^2\;min^{-1}$ for Nafion 117 and $1.8{\times}10^{-7}\;cm^2\;min^{-1}$ for AMV. Consequently, a VRB single cell using the prepared membrane showed higher energy efficiency (over 80%) of up to 100 cycles compared to the commercial membranes, Nafion 117 (ca. 58%) and AMV (ca. 70%).

대전 지역에서 급성 A형 간염의 유전자형에 따른 임상 특성 고찰

이영우 ( Young Woo Lee ),양현웅 ( Hyeon Woong Yang ),이진아 ( Jin A Lee ),윤기호 ( Ki Ho Yun ),양성은 ( Seong Eun Yang ),이민지 ( Min Ji Lee ),박세영 ( Se Young Park ),김새희 ( Sae Hee Kim ),이향이 ( Hyang Ie Lee ),이윤정 ( Yun Ju 대한내과학회 2011 대한내과학회지 Vol.80 No.5

Background/Aims: Acute viral hepatitis A is a major health problem in Korea and the influx of genotype IIIA is thought to be one reason. We examined the differences in the clinical characteristics and laboratory findings of genotypes IA and IIIA in Daejeon. Methods: From November 2009 to June 2010, 81 patients positive for IgM anti-HAV were enrolled prospectively. The hepatitis A was genotyped using real-time polymerase chain reaction. The clinical characteristics and laboratory results were compared on the Results: The mean patient age was 32.6±7.4 years. The mean hospitalization was 7.7±2.4 days. The patient occupation varied. Clinically, vomiting and diarrhea were relatively more prevalent in genotype IIIA than in IA. Abdominal pain and skin spots were relatively more prevalent in genotype IA than in IIIA. The hemoglobin, peak aspartate aminotransferase (AST) level, and C-reactive protein were statistically higher in genotype IIIA than in IA. The distributions of the peak AST, alanine aminotransferase (ALT) and total bilirubin values tended to be perched in genotype IIIA than in IA. The international normalized ratio (INR) tended to be slightly prolonged in genotype IIIA than in IA. Conclusions: Recently, genotype IIIA of acute viral hepatitis A has become prevalent in Daejeon. Hepatitis A genotype IIIA probably causes worse laboratory abnormalities than genotype IA.

Projection of future climate change impacts on nonpoint source pollution loads for a forest dominant dam watershed by reflecting future vegetation canopy in a Soil and Water Assessment Tool model

Park, Min J.,Park, Jong Y.,Shin, Hyung J.,Lee, Mi S.,Park, Geun A.,Jung, In K.,Kim, Seong J. IWA Publishing 2010 Water Science & Technology Vol.61 No.8

<P>This study is to assess the future impact of climate change on hydrological behavior considering future vegetation canopy prediction and its propagation to nonpoint source pollution (NPS) loads. The SWAT (Soil and Water Assessment Tool) model was used for the assessment. For a forest dominant ChungjuDam watershed of South Korea, the MIROC3.2hires climate data of SRES A1B and B1 scenarios were adopted and downscaled for the watershed. The future vegetation canopy information was projected by the monthly relationship between Terra MODIS (MODerate resolution Imaging Spectroradiometer) LAI (Leaf Area Index) and temperature. The future predicted LAI increased up to 1.9 in 2080s April and October because of the temperature increase 3.6°C and 5.3°C respectively. By reflecting the future LAI changes, the future estimated percent changes of maximum annual dam inflow, SS, T-N, and T-P were + 42.5% in 2080s A1B,−35.6% in 2020s A1B,+73.7% in 2080s A1B and−21.0% in 2080s B1 scenario respectively. The increase of T-N load was from the increase of subsurface lateral flows and the groundwater recharges by the future rainfall increase. The decrease of T-P load was by decrease of sediment load during wet days because the effect of LAI increase is greater than the increase of rainfall.</P>

Effect of Jaeumkanghwatang (JEKHT), a Polyherbal Formula on the Pharmacokinetics Profiles of Tamoxifen in Male SD Rats (1)

Min A Kwak,Soo Jin Park,Sung Hwan Park,Young Joon Lee,Sae Kwang Ku 대한한의학회 2016 대한한의학회지 Vol.37 No.2

Poly(vinylbenzyl chloride-glycidyl methacrylate)/Polyethylene Composite Anion Exchange Membranes for Vanadium Redox Battery Application

Min-A Park,심준목,Se-Kook Park,전재덕,진창수,이기봉,신경희 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.6

Anion exchange membranes for a vanadium redox flow battery (VRB) were prepared by pore-filling on a PE substrate with the copolymerization of vinylbenzyl chloride (VBC) and glycidyl methacrylate (GMA). The ion exchange capacity, water uptake and weight gain ratio were increased with a similar tendency up to 65% of GMA content, indicating that the monomer improved the pore-filling degree and membrane properties. The vanadium ion permeability and open-circuit voltage were also investigated. The permeability of the VG65 membrane was only 1.23 × 10−7 cm2 min−1 compared to 17.9 × 10−7 cm2 min−1 for Nafion 117 and 1.8 × 10−7 cm2 min−1 for AMV. Consequently, a VRB single cell using the prepared membrane showed higher energy efficiency (over 80%) of up to 100 cycles compared to the commercial membranes, Nafion 117 (ca. 58%) and AMV (ca. 70%).

Polyphenolic compounds from Korean <i>Lonicera japonica</i> Thunb. induces apoptosis via AKT and caspase cascade activation in A549 cells

Park, Kwang Il,Park, Hyeonsoo,Nagappan, Arulkumar,Hong, Gyeong Eun,Yumnam, Silvia,Lee, Ho Jeong,Kim, Eun Hee,Lee, Won Sup,Shin, Sung Chul,Kim, Jin A,Lee, Sang Joon,Ma, Jin Yeul,Min, Taesun,Heo, Jeong D.A. Spandidos 2017 Oncology letters Vol.13 No.4

<P><I>Lonicera japonica</I> Thunb. (<I>L. japonica</I> T.) has historically been used in Korean herbal medicine due to its anticancer and protective effects on the respiratory system. In the present study, the polyphenolic compounds in <I>L. japonica</I> T. were investigated using high-performance liquid chromatography coupled with tandem mass spectrometry, and its anticancer effects on A549 non-small-cell lung cancer cells were studied. Polyphenolic compounds potentially inhibit A549 cells in a dose-dependent manner. Flow cytometry and western blot analysis demonstrated that polyphenolic compounds induce apoptosis by regulating the protein expression levels of caspases, poly-(ADP-ribose) polymerase and the B-cell lymphoma-2-associated X-protein/B-cell lymphoma-extra large ratio. Furthermore, polyphenolic compounds inhibited mitochondrial membrane potential activity. Caspase-3 activity was increased in a dose-dependent manner and polyphenolic compounds inhibited the activation of protein kinase B by dephosphorylation. These results suggest that polyphenolic compounds in A549 cells indicate the anticancer activity through the induction of apoptosis.</P>

Effect of Jaeumkanghwatang (JEKHT), a Polyherbal Formula on the Pharmacokinetics Profiles of Tamoxifen in Male SD Rats (2) - Oral Combination Treatment of Tamoxifen 50 mg/kg with JEKHT 100 mg/kg on JEKHT 6-day Repeated Pretreated Rats with 8-day Repeated

Park, Soo Jin,Kwak, Min A,Park, Sung Hwan,Lee, Young Joon,Ku, Sae Kwang Society of Preventive Korean Medicine 2016 대한예방한의학회지 Vol.20 No.2

Objectives : The effects of Jaeumkanghwatang (JEKHT) co-administration on the pharmacokinetics of tamoxifen were observed after oral combination treatment of tamoxifen 50 mg/kg with JEKHT 100 mg/kg on JEKHT 6-day repeated oral pretreated rats with 8-day repeated co-administration to confirm the effects of JEKHT co-administration on the pharmacokinetics of tamoxifen. Methods : Six days after pretreatment of JEKHT 100 mg/kg, tamoxifen 50 mg/kg was co-administered with JEKHT 100 mg/kg, once a day for 8 days within 5 min. The blood were collected at 30 min before administration, 30 min, 1, 2, 3, 4, 6, 8 and 24 hrs after end of first and last 8th tamoxifen treatment, and plasma concentrations of tamoxifen were analyzed using LC-MS/MS methods. PK parameters of tamoxifen ($T_{max}$, $C_{max}$, AUC, $t_{1/2}$ and $MRT_{inf}$) were analysis as compared with tamoxifen single administered. Results : Six-day repeated oral pretreatment of JEKHT and 8-day repeated oral co-administration of tamoxifen within 5 min did not influenced on the plasma concentrations and pharmacokinetic parameters of tamoxifen, oral bioavailability, as compared with tamoxifen single treated rats, except for some negligible effects. Conclusions : It is concluded that JEKHT did not influenced on the plasma concentrations and pharmacokinetic parameters, the oral bioavailability of tamoxifen. Therefore, it is considered that co-administration of JEKHT and tamoxifen will be provide an effective novel treatment regimen on the comprehensive and integrative medicine for breast cancer patients, if they showed favorable synergic effects on the pharmacodynamics or reduce the tamoxifen treatment related toxicity and side effects in future studies.

멀티미디어 매체 활용이 한국어 쓰기 능력에 미치는 영향 연구 : 학문 목적 중⋅고급 학습자를 대상으로

신민아, 박소희 배재대학교 주시경교양교육연구소 2017 대학교양교육연구 Vol.2 No.1

최근 한국어를 학습하는 학문 목적 학습자가 점점 증가하고 있으며 이들에게 쓰기 교육은 아주 중요하다고 할 수 있다. 쓰기는 다른 언어 기능과 달리 일상생활에서 자연스럽게 학습할 수 있는 것이 아니라 의도된 교육 환경 아래에서 학습이 가능하다. 이에 멀티미디어매체를 통해 쓰기에 대한 학습자들의 부담을 줄이고 흥미를 유발할 수 있는 쓰기 교육이 필요하다. 본 연구는 부산 소재의 D대학교에서 외국어를 학습하는 중급 24명의 학생을 대상으로 하였다.5) 본 연구에서는 같은 주제에 대한 쓰기를 2회 실시한다. 1회차 쓰기에서 학습자들은 교사의 설명이나 자료의 제공 없이 자신의 생각을 쓴다. 쓰기 수행이 끝나면 학습자는 관련 멀티미디어 매체를 보면서 쓰기에 사용할 어휘나 문장, 내용을 메모한다. 그리고 다시 같은 주제에 대해 2회차 쓰기를 실시한 후 매체 사용 전⋅후의 쓰기 결과를 비교하였다. 멀티미디어 매체를 활용한 수업은 학습자들의 흥미와 동기를 고취시키고 실제적인 정보를 제공한다. 멀티미디어 매체를 통해 얻은 정보와 자료는 쓰기의 중요한 재료가 되며, 이는 쓰기 활동에 긍정적 영향을 미친다. 또한 멀티미디어 매체는 수업 후에도 반복해서 듣고 쓸 수 있는 장점이 있으므로 학문 목적 학습자들의 쓰기 능력 향상에 도움이 된다. 학습자들은 쓰기에 대한 부담을 많이 느낀다. 그러나 멀티미디어 매체를 본 후 쓰기에서 는 내용 구성이 풍부해진 것을 볼 수 있었으며, 쓰기 후 설문조사를 통해 쓰기에 대한 부담감이 줄어든 것을 볼 수 있었다. 멀티미디어 매체를 활용한 쓰기 수업은 첫째, 학습자의 흥미를 유발하며 쓰기에 대한 부담감을 줄이는 데 도움이 된다. 둘째, 멀티미디어 매체를 활용한 쓰기 수업은 학습자들의 쓰기 능력 향상에 도움이 된다. 매체를 활용한 후 학습자들의 쓰기 자료를 분석한 결과, 주제에 대한 전문 용어의 사용이 증가하였고 내용이 명확하고 풍부해진 것을 알 수 있었다. 쓰기는 표현 영역이다. 학문 목적 학습자들이 주어진 주제에 대해 자신의 생각을 명확하게 글로 표현하기 위해서는 언어의 형태보다는 의미에 초점을 두는 수업이 진행되어야 하며, 장기적인 학습이 이루어지려면 학습자의 능동적인 학습 태도가 필요하다. 멀티미디어매체를 활용한 수업은 이와 같은 요구를 만족시키는 수업 도구가 될 것이며 앞으로의 더 많은 연구를 기대해 본다. The number of Korean learners for academic purposes is on a rise recently and for such learners writing (composition) lessons are of great importance. Unlike other aspects of language learning, writing skills cannot be acquired naturally through one’s daily life but can only be mastered through a purposely designed learning environment/model. Therefore, in order to reduce the burden for the learners and to incite their interest we need writing (composition) classes based on using audio-visual medium. This research was conducted targeting 24 intermediate level students learning a foreign language in D-University, Busan. The students were first asked to write their thoughts on a given topic. However, there were a lot of students who found it challenging to write due to the difficulties in using the correct vocabulary and grammar and constructing proper sentences. Moreover, there were students who were restricted by the lack of background knowledge on the topic. The students were then showed news and documentaries related to the topic and were asked to write their views again. The results from the two situations were then compared. A learning environment based on audio-visual medium not only builds interest and motivation among the students but also provides them with realistic information. The information and data obtained through audio-visual medium can become a useful reference material for the students and has a positive impact on the writing activity. Furthermore, since audio-visual medium can be listened to or used repeatedly even after class, it plays an important role in improving the writing skills of students learning Korean for academic purposes. Korean language learners usually feel burdened by writing. However, after playing the audio-visual material, we could see that their writing became richer in content and the survey conducted after the writing activity also reflected a decline in the burden felt by the students. From these results, we can conclude two things. Firstly, a writing class which utilizes audio-visual medium helps in arousing interest in the students and in reducing the burden felt by them. Secondly, a writing class based on using audio-visual materials helps in improving the writing skills of the learners. The analysis of the material written after the application of audio-visual medium showed an increase in the use of technical terms and more clarity and richness in content. Writing is a method to express oneself. In order to enable the students learning Korean for academic purposes to express their thoughts clearly through composition, instead of a class that focuses on the language form, we need a class that focuses on the meaning/significance. Also, for long-term learning, the students as well need to display an active learning attitude. Since a composition class based on audio-visual medium can act as a useful tool to satisfy such requirements, it would be useful to have more research on this topic in the future.

Effect of Jaeumkanghwatang (JEKHT), a Polyherbal Formula on the Pharmacokinetics Profiles of Tamoxifen in Male SD Rats (1) - Single Oral Combination Treatment of Tamoxifen 50 mg/kg with JEKHT 100 mg/kg within 5 min -

Kwak, Min A,Park, Soo Jin,Park, Sung Hwan,Lee, Young Joon,Ku, Sae Kwang The Society of Korean Medicine 2016 대한한의학회지 Vol.37 No.2

Objectives: The objective of this study was to elucidate the effect of Jaeumkanghwatang (JEKHT) on the plasma concentration and pharmacokinetics of tamoxifen in combination therapy as a process of the comprehensive and integrative medicine against breast cancer. Methods: After 50 mg/kg of tamoxifen treatment, JEKHT 100 mg/kg was orally administered within 5 min. The plasma were collected at 30 min before administration, 30min, 1, 2, 3, 4, 6, 8 and 24 hrs after end of JEKHT treatment, and plasma concentrations of tamoxifen were analyzed using LC-MS/MS methods. PK parameters of tamoxifen ($T_{max}$, $C_{max}$, AUC, $t_{1/2}$ and $MRT_{inf}$) were analysis as compared with tamoxifen single administered rats. Results: JEKHT did not influenced on the plasma concentrations and pharmacokinetics of tamoxifen after single oral co-administration, within 5min except for some negligible effects on plasma concentration. The $T_{max}$, $C_{max}$, AUC, $t_{1/2}$ and $MRT_{inf}$ of tamoxifen in co-administered rats were quite similar to those of tamoxifen single treated rats. Conclusions: Based on the results of the present study, JEKHT did not influenced on the oral bioavailability of tamoxifen, when they were single co-administered within 5min. However, more detail pharmacokinetic studies should be tested to conclude the possibilities that can be used as comprehensive and integrative therapy with JEKHT and tamoxifen for breast cancers, when they were co-administered, like the effects on the pretreatment of JEKHT and after repeat co-administrations.